發(fā)布時間：2018-04-21 16:39

  本文選題：自噬 + 自噬受體蛋白　； 參考：《昆明醫(yī)科大學》2017年碩士論文

【摘要】：[目的]檢測子宮內膜異位癥(EMs)患者血清及異位子宮內膜、在位子宮內膜組織(卵巢子宮內膜異位囊腫)中自噬相關基因視神經病變誘導基因(Optineurin OPTN)、P62蛋白的表達,比較OPTN、P62在子宮內膜異位癥患者與正常對照組中的不同表達,以了解OPTN、P62與子宮內膜異位癥的關系,探討自噬相關基因在子宮內膜異位癥發(fā)病機制中的作用,為病因學尋找依據,為治療尋找新的思路。[方法]1.運用酶聯(lián)免疫法(Elisa)測定被研究者血清中OPTN、P62蛋白的表達。2.運用實時熒光定量PCR法(RT-PCR)測定EMs患者異位子宮內膜、在位子宮內膜組織及正常子宮內膜組織中OPTN、P62 mRNA的表達。3.運用Westren-Blot法測定EMs患者異位子宮內膜、在位子宮內膜組織及正常子宮內膜組織中OPTN、P62蛋白的表達。[結果]1.OPTN蛋白在EMs患者血清中的表達高于正常對照組血清中的表達水平(P0. 05) , P62蛋白在EMs患者血清中的表示低于正常對照組血清中的表達水平(P0. 05) 。2. OPTN mRNA在實驗組的異位子宮內膜組織、在位子宮內膜組織中的表達均低于對照組子宮內膜(P0. 05) ; P62 mRNA在實驗組的異位子宮內膜組織、在位子宮內膜組織中的表達高于對照組子宮內膜(P0. 05);但實驗組的異位子宮內膜組織和在位子宮內膜組織中OPTN mRNA及P62 mRNA的表達為異位組低于在位組,差異無統(tǒng)計學意義(P0.05)。3.OPTN蛋白在實驗組的異位子宮內膜組織、在位子宮內膜組織中的表達均低于對照組子宮內膜(P0. 05) ; P62蛋白在實驗組的異位子宮內膜組織、在位子宮內膜組織中的表達高于對照組子宮內膜(P0. 05),但實驗組的異位子宮內膜組織和在位子宮內膜組織中OPTN蛋白及P62蛋白的表達為異位組高于在位組,差異無統(tǒng)計學意義(P0.05)。[結論]1. EMs患者血清中OPTN蛋白高表達,P62蛋白低表達。2. EMs患者組織中OPTN蛋白、mRNA低表達,P62蛋白、mRNA高表達。3.各組織中自噬活性有所差異:正常子宮內膜組高于EMs患者在位內膜組高于異位子宮內膜組,自噬活性逐漸下降。4.本研究進一步證實了:自噬參與了 EMs的發(fā)生、發(fā)展,組織中OPTN低表達、P62高表達促進局部病灶發(fā)生、存活,血清中OPTN高表達、P62高表達可能是機體受到免疫、炎癥反應等刺激后對自身的一種綜合性保護機制。
[Abstract]:[objective] to detect the expression of Optineurin Optineurin Optineurin (OPTNN) P62 protein in serum and ectopic endometrium and eutopic endometrium (ovarian endometriosis cyst) of patients with endometriosis. To investigate the relationship between OPTNP 62 and endometriosis and to explore the role of autophagy related gene in the pathogenesis of endometriosis, and to find out the etiology of endometriosis by comparing the expression of OPTNP 62 in patients with endometriosis and normal control group, in order to understand the relationship between OPTNP 62 and endometriosis, and to explore the role of autophagy gene in the pathogenesis of endometriosis. To find new ideas for treatment. [methods] 1. Enzyme linked immunosorbent assay (Elisa) was used to detect the expression of OPTNN P62 protein. The expression of OPTNT P62 mRNA in ectopic endometrium, eutopic endometrium and normal endometrium of patients with EMs was detected by real-time fluorescence quantitative PCR assay (RT-PCR). The expression of OPTNP62 protein in ectopic endometrium, eutopic endometrium and normal endometrium of EMs patients was determined by Westren-Blot method. [results] the expression of 1.OPTN protein in serum of EMs patients was higher than that of normal control group (P 0. 0). The expression of P62 protein in serum of EMs patients was lower than that of normal control group. (05) .2. The expression of OPTN mRNA in ectopic endometrium and eutopic endometrium in experimental group was lower than that in control group. The expression of P62 mRNA in ectopic endometrium and eutopic endometrium in experimental group was higher than that in control group. However, the expression of OPTN mRNA and P62 mRNA in ectopic endometrium and eutopic endometrium in the experimental group was lower than that in the eutopic group, and there was no significant difference in the expression of OPTN mRNA and P62 mRNA in the ectopic endometrium of the experimental group. The expression of P0 in eutopic endometrium was lower than that in control group. The expression of P62 protein in ectopic endometrium and eutopic endometrium in experimental group was higher than that in control group. However, the expression of OPTN protein and P62 protein in ectopic endometrium and eutopic endometrium in experimental group was higher than that in eutopic group (P 0.05). [conclusion] 1. High expression of OPTN protein and low expression of P62 protein. 2. 2 in serum of EMs patients. The low expression of OPTN protein P62 protein and the high expression of P62 protein mRNA. 3. 3 in patients with EMs. The autophagy activity of normal endometrium group was higher than that of EMs group, and the autophagy activity of eutopic endometrium group was lower than that of ectopic endometrium group. This study further confirmed that autophagy was involved in the occurrence and development of EMs, the low expression of OPTN and the high expression of P62 promoted the occurrence and survival of local lesions. The high expression of OPTN in serum may be immune to the body. A comprehensive protective mechanism for itself after stimulation such as inflammation.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R711.71

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本文編號：1783237