本文選題：褪黑素 + 體質(zhì)量��； 參考：《中山大學(xué)學(xué)報(bào)(醫(yī)學(xué)科學(xué)版)》2015年04期

【摘要】：【目的】探討孕期使用外源性褪黑素對(duì)子代生長(zhǎng)發(fā)育的影響�！痉椒ā縎D孕鼠共30只,隨機(jī)分成3組,每組10只。褪黑素低劑量組MT-LD組:給予褪黑素10 mg·kg-1·d-1,褪黑素高劑量組MT-HD組:40 mg·kg-1·d-1,對(duì)照組:生理鹽水10 mg·kg-1·d-1處理。待分娩后選擇每窩雌性子代每周測(cè)定其體質(zhì)量平均增長(zhǎng)情況。斷奶后每日測(cè)定陰道開(kāi)口及動(dòng)情周期變化。于性周期穩(wěn)定后測(cè)量每窩雌性子鼠股骨、脛骨的平均長(zhǎng)度;ELISA方法檢測(cè)血清褪黑素、生長(zhǎng)激素(GH)、卵泡刺激素(FSH)、黃體生成素(LH)的平均水平,比較不同處理因素下每窩雌性子代體質(zhì)量、長(zhǎng)骨生長(zhǎng)及性發(fā)育的差異�！窘Y(jié)果】共獲得30窩子鼠,分別為MT-LD 92只,MT-HD 84只,對(duì)照組97只。MT-HD組質(zhì)量在各時(shí)間點(diǎn)均高于其余各組,MT-LD組平均體質(zhì)量高于對(duì)照組(P0.01)。MT-HD組陰道開(kāi)口時(shí)間及出現(xiàn)第一個(gè)動(dòng)情間期時(shí)間較其余兩組顯著提前(P0.01),而MTLD與對(duì)照組間無(wú)明顯差異。MT-HD組血清中FSH、LH、褪黑素、生長(zhǎng)激素平均水平分別較其余兩組高(P0.05),而上述各指標(biāo)在MT-LD組及對(duì)照組見(jiàn)無(wú)統(tǒng)計(jì)學(xué)差異。【結(jié)論】外源性褪黑素作用于孕晚期母鼠,影響子代體質(zhì)量、長(zhǎng)骨生長(zhǎng)及性發(fā)育,并存在劑量依賴關(guān)系,臨床上在孕期使用褪黑素時(shí)應(yīng)重視其對(duì)子代體質(zhì)量、性發(fā)育及激素等的影響。
[Abstract]:[objective] to investigate the effects of exogenous melatonin on the growth and development of offspring during pregnancy. [methods] Thirty SD pregnant rats were randomly divided into 3 groups with 10 rats in each group.MT-LD group was treated with 10 mg kg-1 d -1 of melatonin, 10 mg kg-1 d -1 of MT-HD group with high dose of melatonin, and 10 mg kg-1 d -1 of normal saline in control group.After delivery, the average body mass of each litter of female offspring was measured weekly.The changes of vaginal opening and estrus cycle were measured daily after weaning.The mean length of femur and tibia were measured by Elisa to detect the average levels of serum melatonin (melatonin), growth hormone (GHH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) in femur and tibia after sexual cycle stabilization.The weight, long bone growth and sexual development of female progenies in each litter were compared under different treatments. [results] A total of 30 litters of MT-LD 92 MT-HD 84 mice were obtained.The mean body mass of 97 MT-HD patients in the control group was higher than that in the other MT-LD group at all time points, and the vaginal opening time and the first estrous interval time in the MT-HD group were significantly earlier than those in the other two groups, while the average body mass in the MT-LD group was significantly higher than that in the control group, and between MTLD and the control group, the vaginal opening time and the first estrous interval time were significantly earlier.There was no significant difference in serum FSHLH and melatonin in MT-HD group.The average level of growth hormone was higher than that of the other two groups (P 0.05), but there was no statistical difference between the two groups. [conclusion] exogenous melatonin can affect the weight of offspring, the growth of long bone and the development of sex.The effect of melatonin on the weight, sex development and hormones of the offspring should be paid more attention to in clinical use of melatonin during pregnancy in a dose-dependent manner.
【作者單位】： 中山大學(xué)孫逸仙紀(jì)念醫(yī)院婦產(chǎn)科;
【基金】：廣東省科技計(jì)劃項(xiàng)目(2008A060202012)
【分類號(hào)】：R714.2

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