本文選題：線粒體自噬 + 子癇前期　； 參考：《重慶醫(yī)科大學(xué)學(xué)報》2017年03期

【摘要】：目的:探討胎盤組織中Bcl-2家族的前凋亡因子—腺病毒E1B19 k D相互作用蛋白3(BCL2/adenovirus E1B 19 k D protein-interacting protein 3,BNIP3)的表達及其介導(dǎo)的線粒體自噬在子癇前期(preeclampsia,PE)胎盤滋養(yǎng)細胞凋亡調(diào)控中的作用。方法:應(yīng)用Western blot方法檢測BNIP3和cleaved-caspase3在對照及PE胎盤中的表達差異。以人絨毛外滋養(yǎng)細胞株HTR8/SVneo通過缺氧/復(fù)氧(hypoxia/reoxygenation,H/R)構(gòu)建PE細胞模型。構(gòu)建BNIP3小干擾RNA,轉(zhuǎn)染細胞后,H/R處理各組細胞,DCFH-DA法檢測各組細胞活性氧(reactive oxygen species,ROS)生成,流式細胞儀檢測細胞凋亡。結(jié)果:與正常胎盤相比,在PE胎盤組織中BNIP3表達明顯降低(P=0.015),而凋亡蛋白cleaved-caspase3明顯增加(P=0.019);在PE細胞模型中,BNIP3敲低組較對照組的ROS產(chǎn)量顯著增加(P=0.022),且凋亡增加(P=0.038)。結(jié)論:BNIP3介導(dǎo)的線粒體自噬不足引起ROS清除障礙可能參與PE胎盤滋養(yǎng)細胞過度凋亡的發(fā)生。
[Abstract]:Aim: to investigate the expression of proapoptotic factor-adenovirus E1B19 kD interaction protein 3(BCL2/adenovirus E1B 19 KD protein-interacting protein 3 in placental tissues and the role of mitochondrial autophagy in the regulation of placental trophoblast apoptosis.Methods: Western blot method was used to detect the expression of BNIP3 and cleaved-caspase3 in the control group and PE placenta.The PE cell model was established by hypoxia / reoxygenation H / R with human extracellular trophoblastic cell line HTR8/SVneo.BNIP3 small interfering RNAs were constructed and treated with H / R after transfection. The production of reactive oxygen species-ROSs in each group was detected by DCFH-DA and apoptosis was detected by flow cytometry.Results: compared with the normal placenta, the expression of BNIP3 decreased significantly in the placenta of PE, while the apoptotic protein cleaved-caspase3 increased significantly, and the ROS production in the low knock group of PNIP3 in PE cell model was significantly higher than that in the control group.Conclusion the dysfunction of ROS clearance induced by mitochondrial autophagy induced by 10% BNIP3 may be involved in the excessive apoptosis of PE placental trophoblastic cells.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院產(chǎn)科重慶醫(yī)科大學(xué)中國-加拿大-新西蘭聯(lián)合母胎醫(yī)學(xué)實驗室;
【基金】：國家自然科學(xué)基金面上資助項目(編號:81370732)
【分類號】：R714.244
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本文編號：1765656