本文選題：順鉑 + 紫杉醇��； 參考：《山東醫(yī)藥》2015年23期

【摘要】：目的觀察順鉑(DDP)與紫杉醇(TXL)聯(lián)合應用對宮頸癌小鼠宮頸癌組織Bcl-2、Bax、半胱氨酸蛋白酶9(Caspase-9)表達的影響。方法 32只615小鼠均建立小鼠宮頸癌模型,后隨機分為A組9只、B組9只、C組10只、D組10只。A組小鼠皮下注射生理鹽水0.2 m L,每6天1次。B組小鼠皮下注射DDP 4 mg/(kg·m L),每6天1次。C組小鼠腹腔注射TXL 50 mg/(kg·m L),每周1次。D組小鼠皮下注射DDP 4 mg/(kg·m L),每6天1次;TXL 50 mg/(kg·m L),每周1次;第1次注射時先注射TXL后注射DDP,其后兩藥序貫用藥。采用免疫組化SP法檢測各組Bcl-2、Bax、Caspase-9。結(jié)果 A組宮頸癌組織Bcl-2表達量為6 300.43±458.34、Bax表達量為7 244.22±363.72、Bax/Bcl-2為1.14±0.31、Caspase-9表達量為5 144.22±363.71,B組分別為4 146.56±202.50、6 613.89±237.49、1.59±0.22、6 913.89±337.49,C組分別為5 361.20±367.80、6 210.50±320.25、1.15±0.31、9 310.50±220.25,D組分別為1 950.80±103.31、9 334.40±471.53、4.79±1.34、10 334.40±351.53,A、B、C組分別與D組比較,P均0.05。結(jié)論與單用DDP、TXL比較,DDP聯(lián)合TXL化療可明顯降低Bcl-2水平,增加Bax蛋白水平和激活Caspase-9,從而達到促進宮頸癌細胞凋亡的作用。
[Abstract]:Objective to observe the effect of cisplatin (DDP) combined with paclitaxel (TXL) on the expression of Bcl-2 BaxCaspase-9 in cervical carcinoma of mice.Methods the cervical cancer models were established in 32 615 mice.Then it was randomly divided into group A (n = 9), group C (n = 9), group D (n = 10), group D (n = 10), group D (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n =Mice in group D were subcutaneously injected with DDP 4 mg/(kg mL once a week and treated with TXL 50 mg/(kg mL once every 6 days once a week.At the first injection, TXL was injected first, and then the two drugs were used sequentially.Immunohistochemical SP method was used to detect Caspase-9 of Bcl-2 and Baxia in each group.緇撴灉 A緇勫棰堢檶緇勭粐Bcl-2琛ㄨ揪閲忎負6 300.43鹵458.34,Bax琛ㄨ揪閲忎負7 244.22鹵363.72,Bax/Bcl-2涓，

本文編號：1762350