本文選題：子宮內(nèi)膜異位癥 + 痛經(jīng)�。� 參考：《河北醫(yī)科大學(xué)》2014年博士論文

【摘要】：目的：痛經(jīng)是子宮內(nèi)膜異位癥患者的主要癥狀，嚴(yán)重影響患者的生活質(zhì)量，但其發(fā)生機(jī)制尚不明確，成為目前研究的熱點(diǎn)問(wèn)題。本研究通過(guò)分析TRPV1及其致敏因子NGF、BK/BKB1R、PGF2α在內(nèi)異癥患者血清、在位內(nèi)膜、異位內(nèi)膜的變化，以期闡明內(nèi)異癥痛經(jīng)的部分發(fā)病機(jī)制；動(dòng)物實(shí)驗(yàn)是研究藥物治療本病作用機(jī)制的主要手段，但內(nèi)異癥痛經(jīng)的動(dòng)物模型卻未見(jiàn)報(bào)道，本研究嘗試?yán)媒幌礏ALB/c小鼠建立內(nèi)異癥痛經(jīng)模型；采用補(bǔ)腎溫陽(yáng)化瘀方對(duì)內(nèi)異癥痛經(jīng)模型動(dòng)物進(jìn)行干預(yù)，分析治療前后TRPV1及其致敏因子的變化，進(jìn)一步探討補(bǔ)腎溫陽(yáng)化瘀方治療內(nèi)異癥痛經(jīng)的作用機(jī)制。 方法： 第一部分：TRPV1及其致敏因子與子宮內(nèi)膜異位癥痛經(jīng)關(guān)系的研究 內(nèi)異癥組：選自2012年9月～2013年12月于石家莊市第四醫(yī)院住院并符合內(nèi)異癥納入標(biāo)準(zhǔn)的患者34例；對(duì)照組來(lái)自同期住院的子宮肌瘤無(wú)痛經(jīng)患者16例，所有患者術(shù)前抽取空腹肘靜脈血，術(shù)中取在位及異位內(nèi)膜組織，采用ELISA法檢測(cè)血清NGF、BK、PGF2α的含量，采用免疫組化及Western blot方法檢測(cè)NGF、BKB1R、TRPV1蛋白的表達(dá)，采用Real-timePCR方法檢測(cè)NGFmRNA、BKB1RmRNA、TRPV1mRNA的表達(dá)。 第二部分：子宮內(nèi)膜異位癥痛經(jīng)動(dòng)物模型的建立 將68只清潔級(jí)近交系BALB/c雌性小鼠隨機(jī)分為6組，分別為假手術(shù)組、雌激素+縮宮素組、手術(shù)組、手術(shù)+雌激素組、手術(shù)+縮宮素組、手術(shù)+雌激素+縮宮素組，其中假手術(shù)組、雌激素+縮宮素組每組10只，其余每組12只。除假手術(shù)組、雌激素+縮宮素組外其余各組均采用自體移植法將小鼠自體子宮組織塊移植到腹膜，復(fù)制子宮內(nèi)膜異位癥模型，術(shù)后1~12d采用不同方案誘發(fā)小鼠扭體反應(yīng)，記錄扭體潛伏期及扭體次數(shù)，并取異位灶行HE染色和病理組織學(xué)觀察，篩選建立內(nèi)異癥痛經(jīng)模型的最佳方案。 第三部分：補(bǔ)腎溫陽(yáng)化瘀方對(duì)子宮內(nèi)膜異位癥痛經(jīng)模型小鼠TRPV1及其致敏因子的影響 75只近交系BALB/c雌性小鼠隨機(jī)分為5組：假手術(shù)組、模型組、中藥高劑量組、中藥低劑量組、西藥組，每組15只。除假手術(shù)組外，其余各組均采用手術(shù)＋雌激素+縮宮素建立小鼠內(nèi)異癥痛經(jīng)模型，并于術(shù)后1-12d同時(shí)給藥，于第12天誘發(fā)扭體反應(yīng)，斷頭取血并取子宮及異位灶組織。采用ELISA法、免疫組化法、Western blot方法及Real-time PCR方法檢測(cè)各組TRPV1及其致敏因子的變化。 結(jié)果： 第一部分：TRPV1及其致敏因子與子宮內(nèi)膜異位癥痛經(jīng)關(guān)系的研究 1血清NGF、BK、PGF2α的含量及相關(guān)性分析結(jié)果 1.1血清NGF、BK、PGF2α的含量 內(nèi)異癥患者痛經(jīng)組血清NGF、BK、PGF2α水平較對(duì)照組及無(wú)痛組明顯升高，差異有統(tǒng)計(jì)學(xué)意義(P0.01或P0.05)。 1.2血清NGF、BK、PGF2α含量的相關(guān)性分析 NGF與PGF2α呈正相關(guān)關(guān)系(P0.01)，BK與PGF2α水平亦呈正相關(guān)關(guān)系(P0.01)，NGF與BK之間未發(fā)現(xiàn)相關(guān)關(guān)系(P0.05)。 1.3血清NGF、BK、PGF2α含量與VAS評(píng)分的相關(guān)性分析 NGF與VAS評(píng)分呈正相關(guān)(P0.01)， PGF2α與VAS評(píng)分呈正相關(guān)(P0.01)，BK含量與VAS評(píng)分呈正相關(guān)(P0.01)。 2免疫組化法檢測(cè)在位、異位內(nèi)膜組織中NGF、BKB1R、TRPV1蛋白的表達(dá)結(jié)果 2.1NGF的表達(dá) 在位內(nèi)膜痛經(jīng)組NGF表達(dá)增強(qiáng)，與對(duì)照組及無(wú)痛組相比顯著升高(P0.01，P0.05)；異位內(nèi)膜痛經(jīng)組NGF的表達(dá)顯著高于無(wú)痛組(P0.05)。 2.2BKB1R的表達(dá) 在位內(nèi)膜痛經(jīng)組BKB1R表達(dá)增強(qiáng)，與對(duì)照組及無(wú)痛組相比顯著升高(均為P0.01);異位內(nèi)膜痛經(jīng)組BKB1R的表達(dá)顯著高于無(wú)痛組(P0.01)。 2.3TRPV1的表達(dá) 在位內(nèi)膜痛經(jīng)組TRPV1的表達(dá)增強(qiáng)，與對(duì)照組及無(wú)痛組相比顯著升高(均為P0.01)。異位內(nèi)膜痛經(jīng)組TRPV1的表達(dá)顯著高于無(wú)痛組(P0.01)。 3Western blot法檢測(cè)在位、異位內(nèi)膜組織中NGF、BKB1R、TRPV1蛋白的表達(dá)結(jié)果 3.1在位、異位內(nèi)膜組織中NGF的表達(dá) 在位內(nèi)膜痛經(jīng)組NGF的表達(dá)高于對(duì)照組及無(wú)痛組(均為P0.01)；異位內(nèi)膜痛經(jīng)組NGF的表達(dá)顯著高于無(wú)痛組(P0.01)。 3.2在位、異位內(nèi)膜組織中BKB1R的表達(dá) 在位內(nèi)膜痛經(jīng)組BKB1R的表達(dá)高于對(duì)照組及無(wú)痛組(均為P0.01)。異位內(nèi)膜痛經(jīng)組顯著高于無(wú)痛組(P0.01)。 3.3在位、異位內(nèi)膜組織中TRPV1的表達(dá) 在位內(nèi)膜痛經(jīng)組TRPV1的表達(dá)高于對(duì)照組及無(wú)痛組(均為P0.01)。異位內(nèi)膜痛經(jīng)組TRPV1的表達(dá)顯著高于無(wú)痛組(P0.01)。 3.4NGF、BKB1R、TRPV1蛋白表達(dá)之間的相關(guān)性分析 在位內(nèi)膜、異位內(nèi)膜NGF與BKB1R的蛋白表達(dá)呈正相關(guān)關(guān)系(均為P0.01)，NGF與TRPV1的表達(dá)呈正相關(guān)關(guān)系(P0.05，P0.01)，BKB1R與TRPV1的表達(dá)亦呈正相關(guān)關(guān)系(均為P0.01)。 4Real-time PCR檢測(cè)內(nèi)異癥在位、異位內(nèi)膜組織中NGFmRNA、BKB1RmRNA、TRPV1mRNA的表達(dá)結(jié)果 4.1在位、異位內(nèi)膜組織中NGFmRNA的表達(dá) 在位內(nèi)膜痛經(jīng)組NGFmRNA的表達(dá)顯著高于對(duì)照組及無(wú)痛組(均為P0.01)。異位內(nèi)膜NGF mRNA的表達(dá)：痛經(jīng)組顯著高于無(wú)痛組(P0.01)。 4.2內(nèi)異癥在位、異位內(nèi)膜組織中BKB1RmRNA的表達(dá) 在位內(nèi)膜痛經(jīng)組BKB1RmRNA的表達(dá)顯著高于對(duì)照組及無(wú)痛組(均為P0.01)；異位內(nèi)膜BKB1RmRNA的表達(dá)痛經(jīng)組高于無(wú)痛組(P0.01)。 4.3內(nèi)異癥在位、異位內(nèi)膜組織中TRPV1mRNA的表達(dá) 在位內(nèi)膜痛經(jīng)組TRPV1mRNA的表達(dá)顯著高于對(duì)照組及無(wú)痛組(均為P0.01)；異位內(nèi)膜TRPV1mRNA的表達(dá)：痛經(jīng)組顯著高于無(wú)痛組，差異有統(tǒng)計(jì)學(xué)意義(P0.01)。 4.4NGFmRNA、BKB1RmRNA、TRPV1mRNA表達(dá)之間的相關(guān)性分析 在位內(nèi)膜、異位內(nèi)膜NGFmRNA與BKB1RmRNA的表達(dá)呈正相關(guān)關(guān)系(均為P0.01)，NGFmRNA與TRPV1mRNA的表達(dá)呈正相關(guān)關(guān)系(均為P0.01)， BKB1RmRNA與TRPV1mRNA的表達(dá)亦呈正相關(guān)關(guān)系(均為P0.01)。 4.5NGFmRNA、BKB1RmRNA、TRPV1mRNA表達(dá)與VAS評(píng)分的相關(guān)性分析 在位內(nèi)膜、異位內(nèi)膜NGFmRNA、BKB1RmRNA、TRPV1mRNA的表達(dá)與VAS評(píng)分呈正相關(guān)關(guān)系(均為P0.01)。 第二部分：子宮內(nèi)膜異位癥痛經(jīng)動(dòng)物模型的建立 與手術(shù)+縮宮素組、手術(shù)組相比，手術(shù)+雌激素+縮宮素組、手術(shù)+雌激素組移植物體積明顯增大，差異有統(tǒng)計(jì)學(xué)意義(P0.01)；手術(shù)+雌激素+縮宮素組扭體發(fā)生率100%，雌激素+縮宮素組扭體發(fā)生率80%，手術(shù)+縮宮素組扭體發(fā)生率50%，其余組未出現(xiàn)扭體反應(yīng)，組間差異有統(tǒng)計(jì)學(xué)意義(P0.01)；與手術(shù)+縮宮素組、雌激素+縮宮素組相比，手術(shù)+雌激素+縮宮素組扭體潛伏期明顯縮短(P0.01，P0.05)，扭體次數(shù)明顯增多(P0.01，P0.05)，差異有統(tǒng)計(jì)學(xué)意義。 第三部分補(bǔ)腎溫陽(yáng)化瘀方對(duì)子宮內(nèi)膜異位癥痛經(jīng)模型小鼠TRPV1及其致敏因子的影響 1補(bǔ)腎溫陽(yáng)化瘀方對(duì)內(nèi)異癥痛經(jīng)模型小鼠扭體反應(yīng)的影響與模型組相比，，各給藥組扭體潛伏期明顯延長(zhǎng)(均為P0.01)，扭體次數(shù)顯著減少(P0.05或P0.01)。 2補(bǔ)腎溫陽(yáng)化瘀方對(duì)內(nèi)異癥痛經(jīng)模型小鼠異位灶體積的影響與模型組相比，各給藥后異位灶體積明顯縮小(均為P0.01)。 3補(bǔ)腎溫陽(yáng)化瘀方對(duì)內(nèi)異癥痛經(jīng)模型小鼠血清NGF、BK、PGF2α的影響模型組血清NGF、BK、PGF2α含量顯著高于假手術(shù)組(P0.01，P0.01，P0.05)；與模型組相比，各給藥組NGF、BK、PGF2α含量顯著下降，差異有統(tǒng)計(jì)學(xué)意義(P0.05或P0.01) 4模型小鼠血清NGF、BK、PGF2α含量與扭體反應(yīng)的相關(guān)性分析血清BK、PGF2α、NGF濃度與扭體次數(shù)呈正相關(guān)關(guān)系(均為P0.01)；PGF2α濃度與扭體潛伏期呈負(fù)相關(guān)關(guān)系(P0.05)；NGF、BK與扭體潛伏期之間未發(fā)現(xiàn)相關(guān)關(guān)系(P0.05)；NGF與BK呈正相關(guān)關(guān)系(P0.01)，PGF2α與BK呈正相關(guān)關(guān)系(P0.05)，NGF與PGF2α亦呈正相關(guān)關(guān)系(P0.01)。 5免疫組化法檢測(cè)NGF、BKB1R、TRPV1的表達(dá)結(jié)果模型組子宮NGF、BKB1R、TRPV1表達(dá)顯著高于假手術(shù)組(均為P0.01)；與模型組相比，各給藥組子宮及異位灶NGF、BKB1R、TRPV1表達(dá)顯著降低，差異有統(tǒng)計(jì)學(xué)意義(P0.05或P0.01)。 6Western blot檢測(cè)NGF、BKB1R、TRPV1蛋白的表達(dá)結(jié)果 與假手術(shù)組相比，模型組子宮NGF、BKB1R、TRPV1蛋白表達(dá)增高(均為P0.01)；與模型組相比，各給藥組子宮及異位灶NGF、BKB1R、TRPV1表達(dá)降低(均為P0.01)，且NGF、BKB1R、TRPV1蛋白的表達(dá)存在正相關(guān)關(guān)系(均為P0.01)。 7Real-time PCR檢測(cè)子宮及異位灶NGFmRNA、 BKB1RmRNA、TRPV1mRNA的表達(dá)結(jié)果 與假手術(shù)組相比，模型組子宮NGFmRNA、 BKB1RmRNA、TRPV1mRNA表達(dá)增高(均為P0.01)；與模型組相比，各給藥組子宮及異位灶NGFmRNA、BKB1RmRNA、TRPV1mRNA表達(dá)不同程度下降(P0.01或P0.05)，且NGFmRNA、BKB1RmRNA、TRPV1mRNA的表達(dá)存在正相關(guān)關(guān)系(均為P0.01)。 結(jié)論： 1NGF、BK、PGF2α在內(nèi)異癥患者血清中水平升高，且與痛經(jīng)程度呈正相關(guān)關(guān)系，TRPV1、NGF、BKB1R蛋白在內(nèi)異癥患者在位內(nèi)膜、異位內(nèi)膜中表達(dá)增強(qiáng)，TRPV1mRNA、NGFmRNA、BKB1RmRNA在內(nèi)異癥痛經(jīng)患者在位內(nèi)膜及異位內(nèi)膜中表達(dá)增強(qiáng)，且與痛經(jīng)程度呈正相關(guān)。提示TRPV1及其致敏因子NGF、BK/BKB1R、PGF2α可能在內(nèi)異癥痛經(jīng)的發(fā)生過(guò)程中起重要作用； 2手術(shù)+雌激素+縮宮素組引起的扭體次數(shù)最多，扭體潛伏期最短，方法簡(jiǎn)單易行，可用于內(nèi)異癥痛經(jīng)發(fā)病機(jī)制及藥物治療研究，是建立內(nèi)異癥痛經(jīng)模型的最佳方案； 3補(bǔ)腎溫陽(yáng)化瘀方可以縮小模型小鼠異位灶體積，減少扭體次數(shù)，延長(zhǎng)扭體潛伏期，降調(diào)內(nèi)異癥痛經(jīng)模型TRPV1及其致敏因子的表達(dá)，提示補(bǔ)腎溫陽(yáng)化瘀方可通過(guò)影響TRPV1及其致敏因子發(fā)揮治療內(nèi)異癥痛經(jīng)的作用。
[Abstract]:Objective : To study the changes of serum , in - situ and ectopic endometrium of patients with endometriosis , including NGF , BK / BKB1R and PGF2偽 , in order to clarify the pathogenesis of endometriosis .
Animal experiments were the main methods to study the mechanism of drug therapy . However , the animal model of endometriosis was not reported . The study attempted to establish a model of endometriosis by using inbred BALB / c mice .
The effect mechanism of Bushen Wenyang Huayu Fang on the treatment of endometriosis dysmenorrhea was further investigated by using the model animal of tonifying kidney , warming yang and removing blood stasis to intervene and analyze the changes of TRL and its sensitizing factor before and after treatment .


Method :


Study on the relationship between TRpv1 and its sensitizing factor and endometriosis


Patients with endometriosis : 34 patients who were hospitalized in the Fourth Hospital of Shijiazhuang City from September 2012 to December 2013 and included in the standard .
The expression of NGF , BK and PGF2偽 was detected by ELISA . The expression of NGF , BKB1R , TRpv1 was detected by immunohistochemistry and Western blot . The expression of NGF , BKB1R and TRp1mRNA was detected by immunohistochemistry and Western blot . The expression of NGF , BKB1R mRNA and TRPV1mRNA was detected by Real - time PCR .


Part Two : Establishment of Animal Model of endometriosis


68 clean - grade inbred BALB / c female mice were randomly divided into six groups : sham operation group , estrogen + oxytocin group , operation group , operation + estrogen group , operation + oxytocin group , operation + estrogen plus oxytocin group .


The third part : Effect of Bushen Wenyang Huayu Recipe on TRpv1 and its sensitizer in endometriosis model mice


75 inbred BALB / c female mice were randomly divided into five groups : sham operation group , model group , traditional Chinese medicine high - dose group , low - dose Chinese medicine group and western medicine group .


Results :


Study on the relationship between TRpv1 and its sensitizing factor and endometriosis


Assay results of serum NGF , BK , PGF2偽


1.1 Content of serum NGF , BK , PGF2偽


The levels of NGF , BK and PGF2偽 in the patients with endometriosis were significantly higher than those in the control group and the painless group ( P0.01 or P0.05 ) .


1.2 Correlation analysis of serum NGF , BK and PGF2偽 levels


There was positive correlation between NGF and PGF2偽 ( P0.01 ) . There was positive correlation between BK and PGF2偽 ( P0.01 ) . There was no correlation between NGF and BK ( P0.05 ) .


1.3 Correlation between the content of serum NGF , BK and PGF2偽 and VAS score


There was positive correlation between NGF and VAS score ( P0.01 ) , and PGF2偽 positively correlated with VAS score ( P0.01 ) , and BK content was positively correlated with VAS score ( P0.01 ) .


The expression of NGF , BKB1R and TRpv1 protein in ectopic and ectopic endometrium were detected by immunohistochemistry .


2.1 Expression of NGF


The expression of NGF in the ectopic endometrium increased significantly compared with the control group and the painless group ( P0.01 , P0.05 ) .
The expression of NGF in ectopic endometrium was significantly higher than that in painless group ( P0.05 ) .


2 . Expression of 2BKB1R


Compared with control group and painless group , the expression of BKB1R was significantly higher than that in control group and painless group ( P0.01 ) . The expression of BKB1R in ectopic endometrium was significantly higher than that in painless group ( P0.01 ) .


2.3 Expression of TRpv1


Compared with the control group and the painless group ( P0.01 ) , the expression of TRpv1 in the ectopic endometrium was significantly higher than that in the control group and the painless group ( P0.01 ) .


The expression of NGF , BKB1R and TRpv1 protein in ectopic and ectopic endometrium were detected by Western blot .


3.1 Expression of NGF in situ and ectopic endometrium


The expression of NGF in the endometriosis group was higher than that in the control group and the painless group ( P0.01 ) .
The expression of NGF in ectopic endometrium was significantly higher than that in painless group ( P0.01 ) .


3.2 Expression of BKB1R in situ and ectopic endometrium


The expression of BKB1R in the ectopic endometrium was higher than that in the control group and the painless group ( P0.01 ) . The ectopic endometrium was significantly higher than that in the painless group ( P0.01 ) .


3.3 Expression in place and ectopic endometrium tissue


TRv1 expression was higher in the ectopic endometrium than in the control group and in the painless group ( P0.01 ) . The expression of TRpv1 in the ectopic endometrium was significantly higher than that in the painless group ( P0.01 ) .


Analysis of the correlation between expression of 3 . 4 NGF , BKB1R and TRpv1 protein


There was positive correlation between the expression of NGF and BKB1R in the ectopic endometrium , ectopic endometrium and BKB1R ( P0.01 ) .


The expression of NGFmRNA , BKB1RmRNA and TRPV1mRNA in ectopic and ectopic endometrium tissues by 4Real - time PCR


4.1 Expression of NGFmRNA in situ and ectopic endometrium


The expression of NGFmRNA in the ectopic endometrium was significantly higher than that in the control group and the painless group ( P0.01 ) . The expression of the ectopic endometrium NGF mRNA was significantly higher than that in the painless group ( P0.01 ) .


4.2 Expression of BKB1RmRNA in the place of endometriosis and ectopic endometrium


The expression of BKB1RmRNA was significantly higher than that in control group and painless group ( P0.01 ) .
The expression of BKB1RmRNA in ectopic endometrium was higher than that of painless group ( P0.01 ) .


4.3 Expression of TRPV1mRNA in the place of endometriosis and ectopic endometrium


TRPV1mRNA expression was significantly higher than that in control group and painless group ( P0.01 ) .
The expression of TRPV1mRNA in ectopic endometrium was significantly higher than that in painless group ( P0.01 ) .


4 . Correlation analysis between expression of 4 NGFmRNA , BKB1RmRNA and TRPV1mRNA


There was positive correlation between the expression of mRNA and mRNA of BKB1RmRNA ( P0.01 ) . The expression of NGFmRNA and TRPV1mRNA was positively correlated ( P0.01 ) . The expression of BKB1RmRNA and TRPV1mRNA was positively correlated ( P0.01 ) .


Correlation analysis of 4 . 5NGFmRNA , BKB1RmRNA , TRPV1mRNA expression and VAS score


The expression of NGFmRNA , BKB1RmRNA and TRPV1mRNA was positively correlated with VAS score ( P0.01 ) .


Part Two : Establishment of Animal Model of endometriosis


Compared with the operation group and the operation group , the volume of the operation + estrogen plus oxytocin group and the operation + estrogen group was obviously increased , the difference was significant ( P0.01 ) .
The incidence of writhing was 100 % , the rate of estrogen plus oxytocin group was 80 % , the rate of torsion body was 50 % , and no torsion reaction occurred in the rest group ( P0.01 ) .
Compared with the surgery + oxytocin group and estrogen plus oxytocin group , the latency of the torsion body in the operation + estrogen plus oxytocin group was significantly shortened ( P0.01 , P0.05 ) , and the number of writhing increased significantly ( P0.01 , P0.05 ) . The difference was statistically significant .


The effect of the third part of Bushen Wenyang Huayu Recipe on TRpv1 and its sensitization factor in endometriosis model mice


Compared with the model group , the latent period of writhing was significantly prolonged ( P0.01 ) , and the number of writhing was significantly decreased ( P0.05 or P0.01 ) .


Compared with the model group , the volume of ectopic foci was significantly reduced ( P0.01 ) .


The levels of NGF , BK and PGF2偽 in serum NGF , BK and PGF2偽 in the model group were significantly higher than those in sham operation group ( P0.01 , P0.01 , P0.05 ) .
Compared with model group , the content of NGF , BK and PGF2偽 in each group was significantly decreased ( P0.05 or P0.01 ) .


The serum levels of NGF , BK and PGF2偽 in the model mice were positively correlated with the frequency of writhing reaction ( P0.01 ) .
The concentration of PGF2偽 negatively correlated with the latency of writhing ( P0.05 ) .
There was no correlation between NGF , BK and latency of writhing ( P0.05 ) .
There was positive correlation between NGF and BK ( P0.01 ) . There was positive correlation between PGF2偽 and BK ( P0.05 ) . The relationship between NGF and PGF2偽 was positively correlated ( P0.01 ) .


The expression of NGF , BKB1R , TRpv1 in the model group was significantly higher than that in sham operation group ( P0.01 ) .
Compared with the model group , the expressions of NGF , BKB1R and TRpv1 in the uterus and ectopic foci were significantly lower in each group than in the model group ( P0.05 or P0.01 ) .


Expression of NGF , BKB1R and TRpv1 Protein by Western blot


Compared with the sham operation group , the expression of NGF , BKB1R and TRpv1 in the model group increased ( all P0.01 ) .
Compared with the model group , the expressions of NGF , BKB1R , TRpv1 in the uterus and ectopic foci were decreased ( P0.01 ) , and there was positive correlation between NGF , BKB1R and TRpv1 protein ( all P0.01 ) .


Expression of NGFmRNA , BKB1RmRNA and TRPV1mRNA in uterus and ectopic foci by 7Real - time PCR


Compared with sham operation group , the expression of NGFmRNA , BKB1RmRNA and TRPV1mRNA in model group increased ( P0.01 ) .
Compared with model group , the expression of NGFmRNA , BKB1RmRNA and TRPV1mRNA decreased ( P0.01 or P0.05 ) , and the expression of NGFmRNA , BKB1RmRNA and TRPV1mRNA was positively correlated ( P0.01 ) .


Conclusion :


The expression of TRV1mRNA , NGFmRNA , BKB1RmRNA in the ectopic endometrium and ectopic endometrium of patients with endometriosis was positively correlated with the degree of dysmenorrhea . The expression of TRPV1mRNA , NGFmRNA , BKB1RmRNA in the ectopic endometrium and ectopic endometrium was positively correlated with the degree of dysmenorrhea .



2 Surgery + estrogen plus oxytocin group had the most torsion body frequency , the shortest latency was the shortest , the method is simple and easy , it can be used in the pathogenesis of endometriosis and the study of drug therapy , which is the best scheme for the establishment of the model of endometriosis ;



3 Bushen warming yang Huayu can reduce the volume of ectopic foci in the model mice , reduce the number of writhing , prolong the latent period of the writhing , and reduce the expression of the allodynia model TRL 1 and its sensitizing factor in the model mice . It is suggested that the kidney warming yang Huayu can play the role of treating endometriosis by influencing TRpv1 and its sensitizing factor .

【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R711.71

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 邊文會(huì);子宮內(nèi)膜異位癥乏氧緊張狀態(tài)及補(bǔ)腎溫陽(yáng)化瘀法對(duì)其影響[D];河北醫(yī)科大學(xué);2009年

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