本文選題：子宮內(nèi)膜癌 + 微小RNA-d　； 參考：《中山大學(xué)學(xué)報(bào)(醫(yī)學(xué)科學(xué)版)》2017年05期

【摘要】：【目的】研究microRNA-320d(miR-320d)對(duì)子宮內(nèi)膜癌JEC細(xì)胞上皮-間質(zhì)轉(zhuǎn)化功能的影響及其相關(guān)機(jī)制�！痉椒ā縅EC子宮內(nèi)膜癌細(xì)胞株分別轉(zhuǎn)染miR-320d mimics和negative control mimic,分別作為M320d、NCM組,并設(shè)立未轉(zhuǎn)染對(duì)照control組,采用RT-PCR法檢測(cè)各組細(xì)胞miR-320d含量。Transwell實(shí)驗(yàn)檢測(cè)3組細(xì)胞遷移能力和侵襲能力。Westernblot法檢測(cè)3組細(xì)胞α-Catenin和PBX3表達(dá)水平,及PBX3過(guò)表達(dá)對(duì)miR-320d抑制EMT的拮抗作用。雙熒光素酶實(shí)驗(yàn)檢測(cè)miR-320d與PBX3的關(guān)系�！窘Y(jié)果】M320d組miR-320d的表達(dá)水平為control組的(808.25±15.58)倍(P0.05)。M320d組遷移細(xì)胞數(shù)量29.56±0.59低于control組的94.48±1.02(P0.05)。M320d組侵襲細(xì)胞數(shù)量7.33±0.84低于control組的86.28±3.51(P0.05)。M320d組細(xì)胞α-Catenin、E-cadherin蛋白表達(dá)量(0.365±0.017、0.261±0.008)高于對(duì)照組(0.114±0.010、0.151±0.021),Vimentin蛋白表達(dá)量(0.105±0.009)低于對(duì)照組(0.211±0.025),PBX3蛋白表達(dá)量(0.181±0.002)低于對(duì)照組(0.311±0.007)。PBX3過(guò)表達(dá)M320d組子宮內(nèi)膜癌細(xì)胞中α-Catenin、E-cadherin蛋白表達(dá)量(0.139±0.019、0.143±0.007)低于對(duì)照組(0.399±0.034、0.261±0.017),Vimentin蛋白表達(dá)量(0.234±0.008)高于對(duì)照組(0.105±0.009),差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。雙熒光素酶檢驗(yàn)結(jié)果顯示PBX3為miR-320d的下游靶基因�！窘Y(jié)論】miR-320d可能通過(guò)降低下游靶基因PBX3水平影響EMT相關(guān)蛋白表達(dá),抑制子宮內(nèi)膜癌JEC細(xì)胞的上皮-間質(zhì)轉(zhuǎn)化功能。
[Abstract]:[objective] to study the effect of microRNA-320dnmiR-320d on the epithelial-interstitial transformation of endometrial carcinoma JEC cells and its related mechanisms. [methods] JEC endometrial cancer cell lines were transfected into miR-320d mimics and negative control mimic. respectively as M320dNCM group, and untransfected control control group was established.The expression of 偽 -Catenin and PBX3 in the three groups was detected by RT-PCR assay, and the expression of 偽 -Catenin and PBX3 was detected by Western blot, and the antagonistic effect of PBX3 overexpression on the inhibition of EMT by miR-320d.Double luciferase assay was used to detect the relationship between miR-320d and PBX3. [results] the expression level of miR-320d in M320d group was 808.25 鹵15.58 in control group. The number of migration cells in M320d group was 29.56 鹵0.59 lower than that in control group (94.48 鹵1.02(P0.05).M320d group, 7.33 鹵0.84), and that in control group (86.28 鹵3.51(P0.05).M320d group, 86.28 鹵3.51(P0.05).M320d group), the expression of 偽 -Cateninine E-cadherin protein table was lower than that in control group (29.56 鹵0.59).The difference was statistically significant (P 0.05).The results of double luciferase assay showed that PBX3 was the downstream target gene of miR-320d. [conclusion] miR-320d may affect the expression of EMT related protein by decreasing the PBX3 level of downstream target gene and inhibit the epithelial-interstitial transformation function of JEC cells in endometrial carcinoma.
【作者單位】： 中山大學(xué)附屬第一醫(yī)院婦產(chǎn)科;
【基金】：國(guó)家自然科學(xué)青年基金(81602723) 廣東省科技發(fā)展專(zhuān)項(xiàng)(公益研究與能力建設(shè),2016A020215214) 廣東省醫(yī)學(xué)科研基金(A2015130)
【分類(lèi)號(hào)】：R737.33

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