本文選題：蛻膜自然殺傷細(xì)胞(dNK) + 復(fù)發(fā)性流產(chǎn)(RSA)�。� 參考：《中華生殖與避孕雜志》2017年05期

【摘要】：目的研究早孕期復(fù)發(fā)性流產(chǎn)(RSA)患者與正常早孕婦女的蛻膜自然殺傷細(xì)胞(d NK)對(duì)滋養(yǎng)層細(xì)胞系HTR-8/SVneo侵襲能力的影響。方法收集正常早孕人工流產(chǎn)婦女(正常早孕組,n=10)和不明原因RSA患者(RSA組,n=8)的早孕蛻膜組織,用密度梯度離心法分離出蛻膜淋巴細(xì)胞,免疫磁珠法進(jìn)一步分選出CD56+CD16-NK細(xì)胞,即蛻膜NK細(xì)胞,將蛻膜NK細(xì)胞與HTR-8/SVneo共培養(yǎng)24 h后,通過(guò)MTT法和Transwell侵襲實(shí)驗(yàn)檢測(cè)蛻膜NK細(xì)胞對(duì)滋養(yǎng)層細(xì)胞系HTR8/SVneo黏附及侵襲能力的影響,用Real-time PCR檢測(cè)共培養(yǎng)后HTR-8/SVneo細(xì)胞的MMP-2和MMP-9 mRNA水平的表達(dá)情況。結(jié)果與正常早孕婦女相比,不明原因早孕期RSA患者的蛻膜NK細(xì)胞對(duì)滋養(yǎng)層細(xì)胞系HTR8/SVneo侵襲能力有減弱作用,并使HTR-8/SVneo表達(dá)促侵襲分子MMP-2和MMP-9表達(dá)降低。結(jié)論妊娠早期局部母-胎界面d NK細(xì)胞的功能異�？赡苁菍�(dǎo)致不明原因RSA的一個(gè)原因。
[Abstract]:Objective to study the effect of decidual natural killer (NK) on the invasion of trophoblastic cell line HTR-8/SVneo in patients with recurrent early pregnancy (RSA) and normal early pregnant women.Methods the decidual tissues of women with normal early pregnancy induced abortion (normal early pregnancy group) and unexplained RSA group were collected. Decidual lymphocytes were isolated by density gradient centrifugation, and CD56 CD16-NK cells were further isolated by immunomagnetic beads.Decidual NK cells were co-cultured with HTR-8/SVneo for 24 h. The effects of decidual NK cells on the adhesion and invasion of trophoblastic cell line HTR8/SVneo were detected by MTT and Transwell invasion assay.The expression of MMP-2 and MMP-9 mRNA in co-cultured HTR-8/SVneo cells was detected by Real-time PCR.Results compared with normal early pregnant women, decidua NK cells of unexplained early pregnancy RSA patients decreased the invasiveness of trophoblastic cell line HTR8/SVneo, and decreased the expression of MMP-2 and MMP-9 in HTR-8/SVneo.Conclusion the abnormal function of d NK cells at the maternal-fetal interface in early pregnancy may be one of the causes of unexplained RSA.
【作者單位】： 第四軍醫(yī)大學(xué)唐都醫(yī)院生殖醫(yī)學(xué)中心;第四軍醫(yī)大學(xué)免疫學(xué)教研室;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81370710,81571531)~~
【分類(lèi)號(hào)】：R714.21

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本文編號(hào)：1732184