本文選題：Ezrin　切入點：FAK　出處：《蚌埠醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:在宮頸鱗癌組織上進(jìn)行Ezrin及FAK蛋白的檢測,并評估兩種蛋白在宮頸鱗癌中的表達(dá)及意義。方法:在30例正常宮頸(Normal Cervix,NC)、30例低級別鱗狀上皮內(nèi)病變宮頸(Low grade squamous intraepithelial lesions cervix,LSIL)、30例高級別鱗狀上皮內(nèi)病變宮頸(High grade squamous intraepithelial lesions cervix,HSIL)和60例宮頸鱗癌(Squamous carcinoma of the cervix,SCC)組織上,利用QDs標(biāo)記的鏈霉親和素復(fù)合物(QDs labeled streptavidin complexes,QDs-SA)能與生物素化二抗Ig G結(jié)合的特點分別檢測Ezrin和FAK的蛋白表達(dá)。利用免疫組化SP法檢測宮頸鱗癌組織中Ezrin和FAK的表達(dá)水平。結(jié)果:1.SP法觀察到Ezrin、FAK蛋白分別著色于宮頸細(xì)胞的胞膜和胞漿,呈棕黃色,且在宮頸鱗癌細(xì)胞中陽性表達(dá)率分別為75%、80%,與量子點免疫熒光法(QDs-IHC法)結(jié)果相比較,差異均有統(tǒng)計學(xué)意義(P0.05);2.QDs-IHC法觀察到Ezrin、FAK蛋白分別定位于宮頸細(xì)胞的胞膜和胞漿,定位準(zhǔn)確,并且陽性信號分別呈現(xiàn)綠色、橙紅色的光;3.60例SCC組織中Ezrin蛋白及FAK蛋白陽性表達(dá)率分別為96.66%及95.00%,與HSIL組相比差異有統(tǒng)計學(xué)意義(P0.05)。30例HSIL組織中Ezrin和FAK蛋白陽性表達(dá)率分別為83.33%和80.00%,與LSIL組相比差異有統(tǒng)計學(xué)意義(P0.05)。30例LSIL組織中Ezrin和FAK蛋白陽性表達(dá)率分別為60.00%和53.33%,與正常宮頸組相比差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論:Ezrin及FAK的陽性過表達(dá)在宮頸鱗癌的發(fā)生發(fā)展和侵襲過程中具有一定臨床意義,值得深入研究。QDs-IHC法同時檢測多個指標(biāo)優(yōu)于SP法。
[Abstract]:Objective: to investigate the expression of Ezrin and FAK in cervical squamous cell carcinoma (SCC).The protein expression of Ezrin and FAK were detected by QDs labeled labeled streptavidin complex QDs-SAs and biotinylated second antibody Ig G, respectively.Immunohistochemical SP method was used to detect the expression of Ezrin and FAK in cervical squamous cell carcinoma.Results 1. The Ezrininus FAK protein was stained in the cell membrane and cytoplasm of cervix, and the positive expression rate of Ezrinn FAK protein in cervical squamous cell carcinoma cells was 75 and 80, respectively, compared with the QDs-IHC method by quantum dot immunofluorescence (QDs-IHC).The difference was statistically significant. 2.QDs-IHC method showed that Ezrinus FAK protein was located in the membrane and cytoplasm of cervical cells, and the positive signal was green.The positive expression rates of Ezrin protein and FAK protein were 96.66% and 95.00 in orange-red SCC tissues, respectively. The positive expression rates of Ezrin and FAK protein in HSIL tissues were 83.33% and 80.005%, respectively, which were lower than those in LSIL group.The positive expression rates of Ezrin and FAK protein in 30 cases of LSIL were 60.00% and 53.33, respectively. There was no significant difference compared with normal cervix group (P 0.05).Conclusion the positive overexpression of Ezrin and FAK has a certain clinical significance in the development and invasion of squamous cell carcinoma of cervix, and it is worth further studying that the method of QDs-IHC can detect many indexes at the same time, which is superior to SP method.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.33

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本文編號：1726092