本文選題：稽留流產(chǎn)　切入點(diǎn)：E-Cad　出處：《廣西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的:研究上皮型鈣黏附蛋白（E-Cad）在稽留流產(chǎn)患者血清、絨毛和蛻膜組織的表達(dá)，探討稽留流產(chǎn)的發(fā)病機(jī)制，為其早期診斷和臨床防治提供新的理論基礎(chǔ)及實(shí)驗(yàn)依據(jù)。 方法:收集2013年8月至2013年11月我院計(jì)劃生育科病房收治的稽留流產(chǎn)患者25例（實(shí)驗(yàn)組），同期在計(jì)生科門診就診要求終止妊娠的正常早孕婦女25例（對照組），兩組行人工流產(chǎn)術(shù)前均抽血，ELISA法檢測血清中E-Cad的表達(dá)水平；人工流產(chǎn)術(shù)后分別收集兩組絨毛和蛻膜新鮮組織，采取免疫組織化學(xué)方法對兩組絨毛及蛻膜組織E-Cad蛋白表達(dá)進(jìn)行組織學(xué)定位和定量分析。 結(jié)果:稽留流產(chǎn)組孕婦血清E-Cad的濃度為（2.96±0.51）ng/mL，顯著高于對照組（2.02±0.43）ng/mL，兩組比較差異有統(tǒng)計(jì)學(xué)意義（P0.0001）。在絨毛組織，E-Cad主要表達(dá)于滋養(yǎng)細(xì)胞胞膜上，稽留流產(chǎn)組絨毛組織E-Cad蛋白表達(dá)水平高于正常早孕組，兩組比較差異有統(tǒng)計(jì)學(xué)意義(P＜0.0001)。E-Cad主要表達(dá)于蛻膜腺體細(xì)胞的胞膜上，蛻膜間質(zhì)細(xì)胞未見陽性表達(dá)，稽留流產(chǎn)組蛻膜E-Cad蛋白表達(dá)水平高于正常早孕組，兩組比較差異有統(tǒng)計(jì)學(xué)意義(P＜0.0001)。 結(jié)論:稽留流產(chǎn)患者血清、絨毛和蛻膜組織E-Cad的表達(dá)水平均增高，提示稽留流產(chǎn)的發(fā)生可能與E-Cad增高有關(guān)，機(jī)制可能是異常高表達(dá)的E-Cad致使滋養(yǎng)細(xì)胞的侵襲能力下降，，影響滋養(yǎng)細(xì)胞的分化增殖，胎盤著床和血管重鑄障礙，導(dǎo)致流產(chǎn)的發(fā)生。
[Abstract]:Objective: to study the expression of E-Cadc in serum, villi and decidua of patients with missed abortion, to explore the pathogenesis of missed abortion, and to provide a new theoretical and experimental basis for its early diagnosis, clinical prevention and treatment.Methods: from August 2013 to November 2013, 25 patients with missed abortion in family planning ward of our hospital (experimental group) and 25 normal early pregnant women (control group, control group) who requested termination of pregnancy in the outpatient clinic of family planning department were collected from August 2013 to November 2013.The expression of E-Cad in serum was detected by Elisa before artificial abortion.The expression of E-Cad protein in chorionic villi and decidua tissues of the two groups were detected by immunohistochemical method. The expression of E-Cad protein in the chorionic villi and decidua tissues of the two groups were quantitatively analyzed by immunohistochemical method.Results: the serum E-Cad concentration of pregnant women with missed abortion was 2.96 鹵0.51 ng / mL, which was significantly higher than that of control group (2.02 鹵0.43 ng / mL). The difference between the two groups was statistically significant (P 0.0001).The expression of E-Cad in chorionic villi was mainly expressed on trophoblast membrane, and the expression of E-Cad protein in chorionic villi of missed abortion group was higher than that in normal early pregnancy group. The difference between the two groups was significant (P < 0.05). The expression of E-Cad was mainly expressed on the membrane of decidua glandular cells.No positive expression was found in decidual stromal cells, and the expression of decidual E-Cad protein in missed abortion group was higher than that in normal early pregnancy group (P < 0.0001).Conclusion: the expression of E-Cad in serum, villi and decidua of patients with missed abortion is increased, suggesting that the occurrence of missed abortion may be related to the increase of E-Cad, and the mechanism may be that the abnormal high expression of E-Cad can decrease the invasion ability of trophoblastic cells.It affects the differentiation and proliferation of trophoblastic cells, placental implantation and vascular remodeling, leading to miscarriage.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R714.21

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