發(fā)布時(shí)間：2018-04-05 22:04

  本文選題：HPV　切入點(diǎn)：快速篩查　出處：《西北大學(xué)》2014年碩士論文

【摘要】：人乳頭瘤病毒(human papillomavirus,HPV)已被證實(shí)與生殖道疣,口咽部腫瘤,特別是子宮頸癌密切相關(guān)。人體的表皮和黏膜組織對該病毒普遍易感,甚至幾乎所有的宮頸癌患者都有不同程度的HPV感染,因此HPV的分型檢測在宮頸癌早期診斷中有重要意義。 目前已經(jīng)鑒定的HPV達(dá)100多個(gè)型別,根據(jù)流行病學(xué)又可分為高危型和低危型兩種,引起子宮頸癌最常見的高危型有HPV16,18,31,33,35,45,52和58型,HPV6和11型為最常見的低危型,通常與生殖道肛門的疣狀病變有關(guān)。本研究選擇了亞洲地區(qū)高發(fā)的7種高危型(HPV16,18,31,33,45,52,58)和2種低危型(HPV6,11)作為研究對象,建立了兩種HPV新型檢測方法,即PCR-免疫層析檢測法和環(huán)介導(dǎo)等溫?cái)U(kuò)增分型檢測法。分別應(yīng)用這兩種方法對宮頸刷片樣本初步進(jìn)行了篩查分型,并將結(jié)果與醫(yī)院中普遍使用的達(dá)安HPV分型檢測試劑盒進(jìn)行了對比。 所建立的檢測方法與傳統(tǒng)方法相比,具有特異性強(qiáng),靈敏度高,準(zhǔn)確度好,成本低廉等優(yōu)點(diǎn)。在得到臨床樣本后,只需經(jīng)過病毒基因組DNA的粗提,之后通過PCR-免疫層析法的初步篩查后,再對其中的陽性樣本進(jìn)行LAMP分型檢測。完成全部過程大約需要3.5個(gè)小時(shí),在時(shí)間和成本上大大優(yōu)于其它HPV分型檢測方法,在HPV早期診斷方面有很好的應(yīng)用價(jià)值。
[Abstract]:Human papillomavirus (HPV) has been proved to be closely associated with genital warts, oropharyngeal neoplasms, especially cervical cancer.Human epidermis and mucosal tissue are generally susceptible to the virus, even almost all patients with cervical cancer have varying degrees of HPV infection, so the detection of HPV typing is of great significance in the early diagnosis of cervical cancer.At present, more than 100 types of HPV have been identified. According to epidemiology, they can be divided into two types: high risk type and low risk type. The most common high risk types causing cervical cancer are HPV16 / 1831 / 35 / 35 / 455 / 52 and HPV6 / 11 / 58, which are the most common low risk types of cervical cancer.It is usually associated with verrucous lesions in the genital tract and anus.The two methods were used to screen and type the samples of cervical brushes, and the results were compared with the HPV typing kit commonly used in hospitals.Compared with the traditional method, the established method has the advantages of high specificity, high sensitivity, good accuracy and low cost.After the clinical samples were obtained, only the virus genome DNA was extracted, then the positive samples were screened by PCR- immunochromatographic method, and the positive samples were detected by LAMP typing.It takes about 3.5 hours to complete the whole process, which is much better than other HPV typing methods in time and cost, and has a good application value in the early diagnosis of HPV.
【學(xué)位授予單位】：西北大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.33

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本文編號：1716658