本文選題：人巨細(xì)胞病毒　切入點(diǎn)：宮內(nèi)感染　出處：《安徽醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的探討宮內(nèi)人巨細(xì)胞病毒（Human Cytomegalovirus，HCMV）感染對(duì)新生大鼠神經(jīng)行為發(fā)育及遠(yuǎn)期學(xué)習(xí)記憶、運(yùn)動(dòng)和情緒的影響。方法建立宮內(nèi)HCMV感染SD大鼠模型。隨機(jī)從實(shí)驗(yàn)組及對(duì)照組抽取存活的子代大鼠各10只。監(jiān)測(cè)子代大鼠嬰兒期神經(jīng)行為發(fā)育，包括生理發(fā)育：體重增長(zhǎng)、開眼、豎耳；神經(jīng)發(fā)育：聽覺驚愕試驗(yàn)、懸崖回避試驗(yàn)、負(fù)趨地性試驗(yàn)、前肢握力試驗(yàn)及平面翻正試驗(yàn)；于1月齡、3月齡和6月齡時(shí)分別對(duì)實(shí)驗(yàn)組與對(duì)照組進(jìn)行Morris水迷宮、懸吊實(shí)驗(yàn)、曠場(chǎng)實(shí)驗(yàn)等神經(jīng)行為學(xué)檢測(cè)，實(shí)驗(yàn)中從兩組中隨機(jī)選取1月齡時(shí)、6月齡時(shí)子代大鼠，分別采用光鏡、電鏡觀察兩組腦組織病理動(dòng)態(tài)變化特點(diǎn)。結(jié)果1、與正常對(duì)照組比較，實(shí)驗(yàn)組新生大鼠生理發(fā)育遲緩，表現(xiàn)為體重增長(zhǎng)緩慢；開眼、張耳達(dá)標(biāo)率低，差異均有統(tǒng)計(jì)學(xué)意義(P0.05)；神經(jīng)發(fā)育延遲，與正常對(duì)照組比較，表現(xiàn)為聽覺驚愕試驗(yàn)、負(fù)趨地性試驗(yàn)達(dá)標(biāo)率低；懸崖回避試驗(yàn)、平面翻正試驗(yàn)時(shí)間明顯延遲；前肢握力試驗(yàn)時(shí)間縮短，差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。2、學(xué)習(xí)記憶方面：實(shí)驗(yàn)組1月齡、3月齡、6月齡時(shí)的Morris水迷宮逃避潛伏期、游泳距離均顯著高于對(duì)照組,同時(shí)其穿越平臺(tái)次數(shù)顯著低于對(duì)照組，且隨著年齡增加，其穿越平臺(tái)能力下降越明顯，均有統(tǒng)計(jì)學(xué)差異(P 0.01)；運(yùn)動(dòng)能力方面：實(shí)驗(yàn)組大鼠1月齡、3月齡、6月齡時(shí)，懸吊實(shí)驗(yàn)得分均顯著低于對(duì)照組，，差異具有統(tǒng)計(jì)學(xué)意義（P0.001）；情感行為方面：曠場(chǎng)試驗(yàn)結(jié)果：實(shí)驗(yàn)組大鼠1月齡、3月齡、6月齡時(shí)，跨格子數(shù)、站立次數(shù)均顯著低于對(duì)照組，差異具有顯著性（P0.05）。3、實(shí)驗(yàn)組新生大鼠生后1月齡時(shí)，海馬層輕度損傷、神經(jīng)髓鞘輕度溶解，6月齡時(shí)損傷加重，即海馬結(jié)構(gòu)明顯紊亂，髓鞘嚴(yán)重變形、溶解顯著。結(jié)論1、宮內(nèi)HCMV感染新生大鼠嬰兒期神經(jīng)行為發(fā)育較對(duì)照組明顯遲緩。2、宮內(nèi)HCMV感染子代大鼠遠(yuǎn)期空間學(xué)習(xí)記憶功能明顯受損，同時(shí)伴有運(yùn)動(dòng)和情緒行為發(fā)育的異常，且宮內(nèi)HCMV感染子代大鼠神經(jīng)行為受損程度與腦組織病理變化一致。
[Abstract]:Objective to investigate the effects of intrauterine human cytomegalovirus (HCMV) infection on neurobehavioral development, long-term learning and memory, motor and emotion in neonatal rats.Methods SD rat model of intrauterine HCMV infection was established.Ten surviving offspring rats were randomly selected from the experimental group and the control group.To monitor the neurobehavioral development in infant rats, including physiological development: weight gain, open eyes, vertical ears, nerve development: auditory consternation test, cliff avoidance test, negative earth-tendency test, forelimb grip strength test and plane-reversal test.Morris water maze, suspension test, open field test and other neurobehavioral tests were performed in the experimental group and the control group at the age of 3 months and 6 months respectively. The offspring rats of 1 month old and 6 month old were randomly selected from the two groups.The pathological changes of brain tissue in two groups were observed by light microscope and electron microscope respectively.Results 1.Compared with the normal control group, the neonatal rats in the experimental group showed slow weight growth, low open eyes, low ear opening rate and significant difference (P 0.05), delayed nerve development, and compared with the normal control group.The results were as follows: auditory consternation test, negative earth-tendency test, low achievement rate, cliff avoidance test, plane inversion test time obviously delayed, forelimb grip force test time shortened,In learning and memory, the escape latency and swimming distance of Morris water maze in the experimental group were significantly higher than those in the control group at the age of 3 months and 6 months, and the times of crossing the platform were significantly lower than those in the control group.With the increase of age, the ability of traversing platform decreased significantly (P 0.01), and the motor ability of the experimental group was significantly lower than that of the control group at the age of 1 month and 3 months and 6 months old, and the scores of the experimental group were significantly lower than those of the control group, and that of the experimental group was significantly lower than that of the control group.The difference was statistically significant (P 0.001); emotional behavior: the results of open field test showed that when the rats in the experimental group were 3 months old and 6 months old, the number of cross grids and the number of standing times were significantly lower than those in the control group.The difference was significant (P 0.05). At the age of 1 month after birth, the hippocampal layer was slightly damaged in the experimental group, and the injury was aggravated at the age of 6 months after the slight dissolution of the myelin sheath, that is, the structure of the hippocampus was obviously disordered, the myelin sheath was seriously deformed and the dissolution was significant.Conclusion 1. The neurobehavioral development of neonatal rats infected with intrauterine HCMV was significantly slower than that of the control group, and the long-term spatial learning and memory function was significantly impaired in the offspring of intrauterine HCMV infection, accompanied by abnormal motor and emotional behavior development.The degree of neurobehavioral impairment in intrauterine HCMV infected offspring was consistent with the pathological changes of brain tissue.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R714.251

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