本文選題：IL-6　切入點(diǎn)：宮頸上皮內(nèi)瘤變　出處：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:研究白細(xì)胞介素6(IL-6)在不同病變級別的宮頸上皮內(nèi)瘤變和宮頸癌中的表達(dá),分析IL-6在宮頸病變進(jìn)展中的作用以及作為臨床預(yù)警指標(biāo)的實(shí)用價(jià)值。方法:收集2014年8月到2017年2月山西醫(yī)科大學(xué)第二醫(yī)院婦科陰道鏡室177例患者的血清、陰道灌洗液及宮頸組織活檢樣本,其中慢性宮頸炎45例,低度鱗狀上皮內(nèi)瘤變(LSIL)45例,高度鱗狀上皮內(nèi)瘤變(HSIL)47例,宮頸癌40例,用于本研究。采用酶聯(lián)免疫吸附試驗(yàn)(ELISA)雙抗體夾心法檢測IL-6蛋白在不同組別患者的血清、宮頸組織局部微環(huán)境及組織中的表達(dá)。采用熒光PCR(RT-PCR)定量檢測IL-6在組織中的表達(dá),計(jì)算其相對表達(dá)量。結(jié)果:ELISA結(jié)果顯示:血清檢測中,慢性宮頸炎組的IL-6濃度雖低于宮頸癌組,但高于其他癌前病變組,呈現(xiàn)相對較高的水平,組間比較結(jié)果示,除外慢性宮頸炎組和HSIL組無統(tǒng)計(jì)學(xué)差異,其余各組兩兩比較均有統(tǒng)計(jì)學(xué)差異;分析血清中IL-6蛋白濃度與宮頸上皮內(nèi)瘤變和宮頸癌的關(guān)系結(jié)果顯示,除外慢性宮頸炎組,LISL、HSIL、宮頸癌的IL-6蛋白濃度隨病變程度的升高而呈現(xiàn)遞增的趨勢。ELISA結(jié)果顯示:組織局部微環(huán)境檢測中,慢性宮頸炎所測IL-6濃度最低,宮頸癌組最高,組間比較結(jié)果顯示,各組兩兩比較均有統(tǒng)計(jì)學(xué)差異;分析組織中IL-6蛋白濃度與宮頸上皮內(nèi)瘤變和宮頸癌的關(guān)系結(jié)果顯示,相對慢性宮頸炎而言,IL-6蛋白的濃度隨病變級別的升高而呈遞增趨勢;結(jié)合宮頸上皮內(nèi)瘤變和宮頸癌發(fā)生的多種因素,灌洗液IL-6、家族腫瘤史、持續(xù)感染HR-HPV起重要作用;IL-6/IL-2的值隨著病變程度的增加而增大,灌洗液中IL-6與IL-10的相關(guān)性不大。組織中IL-6測定結(jié)果顯示:隨病變程度的增加,組織中IL-6表達(dá)增加,逆轉(zhuǎn)錄熒光定量PCR結(jié)果顯示:組織中IL-6m RNA隨著病變級別的升高而表達(dá)量增加,其中在宮頸癌中表達(dá)量最高,進(jìn)行組間兩兩比較結(jié)果顯示,除LSIL與HSIL兩組間差異無統(tǒng)計(jì)學(xué)意義外,其余各組兩兩比較均有統(tǒng)計(jì)學(xué)差異。分析血清中與組織中IL-6蛋白表達(dá)的相關(guān)性結(jié)果顯示,二者相關(guān)性不大。結(jié)論:慢性宮頸炎和HSIL組血清IL-6病變差異無統(tǒng)計(jì)學(xué)意義,血清IL-6不能指示疾病的發(fā)展變化;隨病變級別升高,宮頸組織局部微環(huán)境中IL-6表達(dá)遞增,可與灌洗液IL-2結(jié)合作為一種預(yù)警指標(biāo)指示宮頸疾病的發(fā)展;組織IL-6表達(dá)有差異,但I(xiàn)L-6m RNA在LSIL組與HSIL組無統(tǒng)計(jì)學(xué)差異,不適合作為預(yù)測CIN發(fā)展的生物指標(biāo)。
[Abstract]:Objective: to study the expression of interleukin-6 (IL-6) in cervical intraepithelial neoplasia and cervical carcinoma with different pathological grades. To analyze the role of IL-6 in the progression of cervical lesions and its practical value as a clinical early warning index. Methods: from August 2014 to February 2017, the serum samples of 177 patients in gynecological colposcopy room of the second Hospital of Shanxi Medical University were collected. Among them, 45 cases were chronic cervicitis, 45 cases were low grade squamous intraepithelial neoplasia, 47 cases were high grade squamous intraepithelial neoplasia, and 40 cases were cervical carcinoma. To detect the expression of IL-6 protein in serum, local microenvironment and tissue of different groups of patients by Elisa. Fluorescence PCR RT-PCR) was used to quantitatively detect the expression of IL-6 in different groups of patients. Results the IL-6 concentration of chronic cervicitis group was lower than that of cervical cancer group, but higher than that of other precancerous lesion group. Except chronic cervicitis group and HSIL group, there was no statistical difference between the two groups, and there were statistical differences in the other groups. The relationship between serum IL-6 protein concentration and cervical intraepithelial neoplasia and cervical cancer was analyzed. Except for the chronic cervicitis group, the IL-6 protein concentration of cervical cancer showed an increasing trend with the increase of pathological degree. Elisa results showed that the IL-6 concentration in chronic cervicitis group was the lowest, and that in cervical cancer group was the highest. The results showed that there were statistical differences between the two groups, and the relationship between the concentration of IL-6 protein and cervical intraepithelial neoplasia and cervical cancer was analyzed. Compared with chronic cervicitis, the concentration of IL-6 protein increased with the increase of pathological grade, combined with many factors of cervical intraepithelial neoplasia and cervical cancer, IL-6 in lavage fluid, family tumor history, The level of IL 6 / IL 2 increased with the increase of pathological degree, but the correlation between IL-6 and IL-10 in the lavage fluid was not significant. The results of IL-6 assay showed that the expression of IL-6 increased with the increase of the degree of lesion. The results of reverse transcription-fluorescence quantitative PCR showed that the expression of IL-6m RNA increased with the increase of pathological grade, and the expression of IL-6m RNA was the highest in cervical cancer. The results of comparison between groups showed that there was no significant difference between the two groups except LSIL and HSIL. The correlation between the expression of IL-6 protein in serum and tissue was not significant. Conclusion: there is no significant difference in serum IL-6 between chronic cervicitis and HSIL. Serum IL-6 can not indicate the development and change of the disease, and the expression of IL-6 in local microenvironment of cervical tissue increases with the increase of pathological grade, which can be combined with lavage fluid IL-2 as a warning indicator to indicate the development of cervical disease, and the expression of IL-6 in tissue is different. However, IL-6m RNA had no statistical difference between LSIL group and HSIL group, so it was not suitable to be used as a biomarker to predict the development of CIN.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.33

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 洪慧;王鶴;賈超穎;黃鶯;;HR-HPV感染后宮頸微環(huán)境中免疫炎癥因子變化[J];安徽醫(yī)學(xué);2014年01期

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本文編號：1695522