本文選題：卵巢癌　切入點：順鉑耐藥　出處：《廣東醫(yī)學(xué)》2017年13期

【摘要】：目的從蛋白質(zhì)水平研究氯化鑭對人卵巢癌順鉑耐藥細(xì)胞(COC1/DDP)功能蛋白表達(dá)的影響,為探討氯化鑭逆轉(zhuǎn)卵巢癌順鉑耐藥的機制提供實驗依據(jù)。方法取對數(shù)生長期COC1/DDP細(xì)胞分為4組,對照組只加入10%胎牛血清培養(yǎng)基培養(yǎng),順鉑組加入23.08μg/m L順鉑,氯化鑭組和氯化鑭+順鉑組分別加入1.5μmol/L氯化鑭,處理8 h后氯化鑭+順鉑組加入23.08μg/m L順鉑。采用蛋白印跡(Western blot)技術(shù)檢測順鉑和氯化鑭分別作用于COC1/DDP后,4組中ERCC1、Ki67、CDK6、HDAC2、c-Cbl和微管末端結(jié)合蛋白1(EB1)6種功能蛋白的表達(dá)變化。結(jié)果與對照組相比,COC1/DDP的4種功能蛋白ERCC1、Ki67、CDK6、c-Cbl在順鉑組和順鉑+氯化鑭組中表達(dá)顯著下調(diào),差異有統(tǒng)計學(xué)意義(P0.05);且順鉑+氯化鑭組較順鉑組表達(dá)明顯下調(diào),差異有統(tǒng)計學(xué)意義(P0.05)。與對照組比較,順鉑組中HDAC2和EB1表達(dá)明顯下調(diào),差異有統(tǒng)計學(xué)意義(P0.05);順鉑+氯化鑭組與順鉑組、氯化鑭組中表達(dá)比較有下降趨勢,但差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論氯化鑭可能通過多種途徑下調(diào)ERCC1、Ki67、CDK6和c-Cbl表達(dá),從而介導(dǎo)逆轉(zhuǎn)卵巢癌順鉑耐藥。
[Abstract]:Objective to study the effect of lanthanum chloride on the expression of functional protein of cisplatin resistant human ovarian cancer cell line (COC1 / DDP) at protein level, and to provide experimental evidence for the mechanism of lanthanum chloride reversing cisplatin resistance in ovarian cancer. Methods COC1/DDP cells at logarithmic growth stage were divided into 4 groups. The control group was cultured in 10% fetal bovine serum medium, the cisplatin group was added 23.08 渭 g / mL cisplatin, the lanthanum chloride group and lanthanum cisplatin group were added 1.5 渭 mol/L lanthanum chloride, respectively. After 8 hours of treatment, lanthanum cisplatin group was added 23.08 渭 g / mL cisplatin. Western blotting technique was used to detect the expression of ERCC1 / Ki67CDK6HDAC2Cbl and microtubule terminal binding protein (1(EB1)6) functional proteins in ERCC1 / Ki67CDK6HDAC2Cbl and microtubule terminal binding protein (1(EB1)6) groups respectively. Compared with the control group, the four functional proteins of COC1 / DDP, ERCC1 / Ki67, CDK6Cbl, were significantly down-regulated in cisplatin and lanthanum chloride groups. The expression of HDAC2 and EB1 in cisplatin lanthanum chloride group was significantly lower than that in cisplatin group, and the difference was statistically significant. Compared with the control group, the expression of HDAC2 and EB1 in cisplatin group was significantly down-regulated. The expression of CDK6 and c-Cbl in lanthanum chloride group was lower than that in cisplatin group and lanthanum chloride group, but the difference was not statistically significant. Conclusion the expression of CDK6 and c-Cbl may be down-regulated by lanthanum chloride. Thus mediated reversal of cisplatin resistance in ovarian cancer.
【作者單位】： 南昌大學(xué)第一附屬醫(yī)院婦產(chǎn)科;
【基金】：國家自然科學(xué)基金資助項目(編號:81260381)
【分類號】：R737.31

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