本文選題：SKOV3/DDP　切入點(diǎn)：低劑量輻射　出處：《青島大學(xué)》2016年碩士論文

【摘要】：目的:順鉑是卵巢癌的主要化療藥物,然而在臨床應(yīng)用中卵巢癌細(xì)胞往往會呈現(xiàn)出對順鉑的耐藥現(xiàn)象。前期研究發(fā)現(xiàn)腫瘤細(xì)胞對低劑量輻射敏感,低劑量輻射治療可以提高卵巢癌對化療藥物的敏感性,但是機(jī)制卻不是很清楚。本課題擬將研究低劑量輻射對卵巢癌順鉑耐藥細(xì)胞的影響,并從Survivin和Caspase-3基因水平,了解低劑量輻射對卵巢癌順鉑耐藥細(xì)胞的影響機(jī)制。方法:卵巢癌順鉑耐藥(SKOV3/DDP)細(xì)胞隨機(jī)分為三組,常規(guī)照射組、低劑量輻射組(LDR)、對照組,每組的順鉑濃度梯度均為0,0.625,1.25,2.5,5和10ug/L。于0,8,16,24時(shí)給予低劑量輻射組0.5Gy照射,照射總量為2Gy。常規(guī)組給予2Gy一次的照射,對照組不給予照射。CCK8用于檢測低劑量輻射對SKOV3/DDP細(xì)胞活性的影響,流式細(xì)胞術(shù)用于檢測腫瘤細(xì)胞的凋亡,real-time PCR技術(shù)檢測腫瘤細(xì)胞耐藥相關(guān)蛋白Caspase-3和Survivin的表達(dá)。結(jié)果:與對照組和常規(guī)組相比,低劑量輻射組的細(xì)胞增殖活性明顯降低(P0.05)。低劑量照射組,對照組和常規(guī)照射組的IC50分別是:3.837±0.16,9.467±0.17和9.389±0.17。低劑量輻射組的凋亡率明顯升高,順鉑濃度為2.5ug/L時(shí),低劑量輻射組的凋亡率為51.40%±0.24,對照組為20.70%±0.59,常規(guī)劑量組為39.20%±0.29。低劑量輻射組的Caspase-3表達(dá)水平明顯高于常規(guī)劑量組、對照組,而Survivin表達(dá)水平低于對照組和常規(guī)照射組(P0.05)。結(jié)論:在卵巢癌組織中Survivin的表達(dá)水平高于正常卵巢組織,Caspase-3的表達(dá)水平低于正常卵巢組織。Survivin和Caspase-3的表達(dá)對卵巢癌的形成有重要的作用。本實(shí)驗(yàn)研究發(fā)現(xiàn)卵巢癌耐藥細(xì)胞經(jīng)低劑量照射后,細(xì)胞中的Survivin的表達(dá)水平降低,Caspase-3的表達(dá)水平升高。我們得出結(jié)論,低劑量輻射或許能夠通過調(diào)節(jié)Survivin和Caspase-3的表達(dá)來增強(qiáng)卵巢癌細(xì)胞對順鉑的敏感性,提高卵巢癌的臨床治療效果,為卵巢癌的臨床治療提供新的方法。
[Abstract]:Objective: cisplatin is the main chemotherapeutic agent for ovarian cancer. However, in clinical application, ovarian cancer cells tend to be resistant to cisplatin. Previous studies have found that tumor cells are sensitive to low dose radiation. Low dose radiation therapy can improve the sensitivity of ovarian cancer to chemotherapeutic drugs, but the mechanism is not very clear. In this study, we will study the effects of low dose radiation on cisplatin resistant ovarian cancer cells, and study the gene levels of Survivin and Caspase-3. To understand the mechanism of low dose radiation on cisplatin resistant ovarian cancer cells methods: ovarian cancer Cisplatin resistant SKOV3 / DDP cells were randomly divided into three groups: conventional radiation group, low dose radiation group, control group. The gradient of cisplatin concentration in each group was 0 ~ 0.625 ~ 1.25 ~ 2.5g / L and 10 g / L 路L ~ (-1). The low dose radiation group was given 0.5Gy irradiation at 24:00 and the total dose was 2Gy.The routine group was given 2Gy once, while the control group was not given the irradiation. CCK8 was used to detect the effect of low dose radiation on the activity of SKOV3/DDP cells. Flow cytometry was used to detect the expression of Caspase-3 and Survivin in tumor cells by real-time PCR. The cell proliferation activity of low dose radiation group was significantly lower than that of low dose radiation group. The IC50 of low dose radiation group, control group and conventional irradiation group were respectively 3. 837 鹵0. 169.467 鹵0. 17 and 9.389 鹵0. 17.The apoptotic rate of low dose radiation group was significantly higher than that of normal irradiation group, when the concentration of cisplatin was 2.5ug/L. The apoptotic rate was 51.40% 鹵0.24 in low dose radiation group, 20.70% 鹵0.59 in control group and 39.20% 鹵0.29 in conventional dose group. The expression of Caspase-3 in low dose radiation group was significantly higher than that in normal dose group and control group. Conclusion: the expression level of Survivin in ovarian cancer is higher than that in normal ovarian tissue. The expression of survivin and Caspase-3 in ovarian cancer is lower than that in normal ovarian tissue. This study found that ovarian cancer drug-resistant cells were exposed to low doses of radiation, We conclude that low dose radiation may enhance the sensitivity of ovarian cancer cells to cisplatin by regulating the expression of Survivin and Caspase-3, and improve the clinical therapeutic effect of ovarian cancer. To provide a new method for the clinical treatment of ovarian cancer.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R737.31

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