本文選題：妊娠期煙熏　切入點：新生大鼠　出處：《新鄉(xiāng)醫(yī)學院》2014年碩士論文

【摘要】：背景眾所周知吸煙成癮已成為這個社會不可忽視的問題,抽煙不僅對吸煙者本人乃至周圍的人甚至子代都產生不良影響。孕期尼古丁暴露會導致胎兒造成極大的危害,如：致畸、宮內缺氧、低體重兒、先天性疾病、嬰兒猝死癥候群等。煙堿是香煙中的主要生物堿,在妊娠期煙堿暴露所致胎兒中樞性呼吸異常中起關鍵作用,而穩(wěn)定的節(jié)律性呼吸對于維持機體正常的生命活動至關重要。因此,研究孕期尼古丁暴露對新生大鼠延髓腦片基本節(jié)律性放電和在體耗氧量的作用至關重要,為進一步明確孕期尼古丁暴露對子代延髓呼吸中樞的抑制作用,并為相關疾病的預防和治療提供新思路和實驗基礎。 目的研究妊娠期煙熏對子代延髓呼吸中樞基本節(jié)律性呼吸放電及延髓呼吸中樞a7nACh受體(a7nicotinic acetylcholinergic receptor, a7nAChR)蛋白和基因表達的變化,探討α7nAChR在孕期煙熏中所致子代呼吸抑制中的作用和機制。 方法0-3天Sprague-Dawley大鼠,雌雄不拘,隨機分為對照組和實驗組。對照組新生大鼠產自自然日夜規(guī)律、常規(guī)飼養(yǎng)的成年大鼠；實驗組新生大鼠產自除常規(guī)飼養(yǎng)外,自合籠至分娩每日2次(8Am和8Pm)、置于80cm*60cm*100cm*煙熏箱中8只香煙煙熏30min的大鼠。 1.用新生大鼠離體延髓腦片標本記錄反映延髓呼吸中樞功能的腦片基本節(jié)律性呼吸放電(rhythmic respiratory discharge activity, RRDA)。觀察α7nAChR激動劑乙酰膽堿(acetylcholine,ACh)和拮抗劑銀環(huán)蛇毒素(Alpha-bungarotoxin,α-BGT)對對照組和實驗組RRDA的作用,分析α7nAChR是否對RRDA有抑制作用,以明確α7nAChR是否參與孕期煙熏對子代的呼吸抑制作用。 2.采用Western Blot技術檢測α7nAChR在延髓呼吸中樞面神經后核內側區(qū)神經元上的表達,明確孕期煙熏是否減少α7nAChR在延髓呼吸中樞面神經后核內側區(qū)神經元上的表達。 3.采用qRT-PCR技術檢測α7nAChR mRNA在延髓呼吸中樞面神經后核內側區(qū)神經元上的表達,明確孕期煙熏是否減少α7nAChR mRNA在延髓呼吸中樞面神經后核內側區(qū)神經元上的表達。分析α7nAChR功能改變與受體和αRNA表達是否一致,是否存在相關性。 結果 1.對照組和實驗組腦片放電60min內穩(wěn)定無衰減,實驗模型穩(wěn)定可靠。實驗組腦片放電弱于對照組(以對照組為100%,實驗組20min時TI88.23±3.17%、IA89.52±4.74%、RC90.06±4.57%)；α7nAChR激動劑ACh對實驗組和對照組腦片放電都有興奮作用,對照組興奮效果強于實驗組(實驗組變化率分別13.05±0.46%、10.37±0.49%、7.65±±±0.40%；對照組為：19.67±0.54%、17.55±0.49%18.04±0.58%)；α7nAChR拮抗劑a-BGT對實驗組和對照組腦片放電都有抑制作用,對照組抑制效果強于實驗組(實驗組變化率分別為9.67±0.50%、8.43±±±0.49%、6.67±0.53%;對照組為：16.65±0.45%、14.92±0.49%、14.33±0.43%)。 2. Western Blot實驗結果顯示,與對照組相比,實驗組α7nAChR在延髓呼吸中樞面神經后核內側區(qū)神經元表達減少(對照組1.012±0.090、實驗組0.604±0.034)。 3qRT-PCR實驗結果顯示,與對照組相比,實驗組α7nAChRmRNA在延髓呼吸中樞面神經后核內側區(qū)神經元表達水平下調(對照組1.004±0.101、實驗組0.809±0.069)。 結論 1.孕期煙熏抑制新生鼠延髓腦片基本節(jié)律性呼吸放電、抑制α7nAChR對RRDA的調節(jié)作用,下調α7nACh受體蛋白和mRNA表達水平。 2.抑制α7nACh受體活性及功能,通過下調α7nACh受體蛋白水平和受體mRNA表達水平使延髓呼吸中樞面神經后核內側區(qū)神經元數量減少、功能下降可能是孕期煙熏抑制子代延髓中樞呼吸功能的機制之一。
[Abstract]:Background : It is well known that smoking addiction has become a non - negligible problem in society . Smoking is not only harmful to smokers themselves or even those around them . The exposure to nicotine in pregnancy plays a key role in fetal central respiratory abnormalities due to exposure to nicotine .

Objective To study the effect and mechanism of 偽 - 7nCR on the respiratory depression in children during pregnancy .

Methods Sprague - Dawley rats were randomly divided into two groups : control group and experimental group .
In the experimental group , the newborn rats were fed twice daily ( 8Am and 8Pm ) except for routine feeding , and were placed in 80 cm * 60 cm * 100 cm * smoking oven for 30 min .

1 . The basic rhythmic respiratory discharge activity ( RRDA ) of the brain slices reflecting the function of the medullary respiratory center was recorded from the specimen of the isolated medulla of the neonatal rat . The effect of 偽 - bungarotoxin ( 偽 - BGT ) on RRDA in the control group and experimental group was investigated .

2 . Western Blot was used to detect the expression of 偽7nCR on the neurons in the medial region of the nucleus of the central facial nerve of the medullary respiratory center .

3 . Using qRT - PCR technique to detect the expression of 偽7nCR mRNA in the medial region of the nucleus of the central facial nerve of the medullary respiratory center .

Results

1 . The experimental model was stable and reliable in the control group and the experimental group , and the experimental model was stable and reliable . The experimental group was weaker than the control group ( 100 % in the control group , TI88.23 鹵 3.17 % , IA89.52 鹵 4.74 % , RC90.06 鹵 4.57 % ) .
In the experimental group and the control group , there was an exciting effect on both the experimental group and the control group . The control group was more excited than the experimental group ( 13.05 鹵 0.46 % , 10.37 鹵 0.49 % , 7.65 鹵 鹵 0.40 % , respectively ) .
The control group was 19.67 鹵 0.54 % , 17.55 鹵 0.49 % 18.04 鹵 0.58 % ;
The results showed that a - BGT inhibited the discharges of brain slices in the experimental group and the control group , and the control group was stronger than the experimental group ( 9.67 鹵 0.50 % , 8.43 鹵 鹵 0.49 % , 6.67 鹵 0.53 % , 14.92 鹵 0.49 % , 14.33 鹵 0.43 % ) .

2 . Compared with the control group , the expression of 偽 - 7nR _ ( R ) R _ ( R ) R _ ( R _ ( R ) R _ ( R ) _ ( R _ ( R ) R _ ( R ) _ ( R _ ( R ) R _ ( R ) _ ( R _ ( R ) _ ( R ) _ ( R _ ( R ) _ ( R ) _ ( R _ ( R ) _ ( R ) _ ( R _ ( R ) _ ( R ) _ ( R _ ( R ) _ ( R ) _ ( R _ ( R ) _ ( R ) _ ( R _ ( R ) _ ( R ) _ ( R _ ( R ) _ ( R ) _ ( R ) in the

The results of 3qRT - PCR showed that the expression of 偽 - 7nCDRmRNA in experimental group was lower than that of control group ( 1.004 鹵 0.101 in the control group and 0.809 鹵 0.069 in the experimental group ) .

Conclusion

1 . During the pregnancy period , the basic rhythmic respiratory discharge was inhibited by smoke suppression in neonatal rats , and the regulatory action of 偽7nCR on RRDA was inhibited , and the expression level of 偽7nAl _ 1 receptor protein and mRNA was downregulated .

2 . The inhibition of the activity and function of 偽 - 7nach receptor , the decrease of the number of neurons in the medial region of the nucleus of the nucleus of the medullary respiratory center after downregulation of the level of the 偽 - 7nabolol receptor protein and the level of receptor mRNA , the decrease in function may be one of the mechanisms for the inhibition of the respiratory function of the nucleus of the medulla of the subnucleus during pregnancy .

【學位授予單位】：新鄉(xiāng)醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R714.2

【參考文獻】

相關期刊論文 前7條

1 張文玲;文亞男;鄭紅;鄧錦波;;酒精暴露與中樞神經系統(tǒng)的損傷[J];河南大學學報(醫(yī)學版);2013年04期

2 李川,余微,鄭煜;前包欽格復合體區(qū)微量注射氨基酸類藥物的呼吸效應[J];生理學報;2000年04期

3 周順長;吳健鴻;張景輝;袁宗輝;曾繁典;;香煙煙霧暴露對中國樹，

本文編號：1676166