本文選題：卵巢切除術(shù)　切入點(diǎn)：雌二醇　出處：《廣西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的觀察雙側(cè)卵巢切除(OVX) SD大鼠不同時(shí)間點(diǎn)血清骨轉(zhuǎn)換標(biāo)記物、骨小梁三維結(jié)構(gòu)動(dòng)態(tài)變化。方法將32只6月齡雌性SD大鼠隨機(jī)分為假手術(shù)(Sham)組和雙側(cè)卵巢切除術(shù)(OVX)組,在術(shù)后選擇4個(gè)時(shí)間點(diǎn)(2、4、8、12周)隨機(jī)選取樣本,每個(gè)組別各時(shí)間點(diǎn)予以處死4只動(dòng)物,抽取血清測(cè)量雌二醇(E2)、骨堿性磷酸酶(BALP)、抗酒石酸酸性磷酸酶-5b (TRACP-5b)濃度,侵入性手術(shù)取脛骨并用顯微CT (Micro-CT)和顯微鏡HE染色方法觀察脛骨骨小梁微結(jié)構(gòu)變化。結(jié)果術(shù)后2周,OVX組E2較Sham組開(kāi)始下降,在第4周到12周時(shí)有明顯差異(P0.05),而Sham組E2水平隨時(shí)間變化不大;OVX組血清BALP在術(shù)后2周到達(dá)峰值后呈現(xiàn)出下降趨勢(shì),在第12周時(shí)下降到術(shù)前的水平;而OVX組的血清TRACP-5B水平在術(shù)后迅速升高到第4周達(dá)峰值后出現(xiàn)下降趨勢(shì),但是在術(shù)后12周時(shí)讓然大于Sham組及術(shù)前水平;用Micro-CT分析脛骨部位干骺端的相對(duì)骨體積(BV/TV)、骨小梁數(shù)目(Tb. N)、骨小梁分離度(Tb.Sp)、Conn. D、骨密度(BMD)在2周時(shí)與Sham組無(wú)明顯差異,第4周開(kāi)始出現(xiàn)明顯差異,12周差異更加明顯,骨小梁厚度(Tb. Th)始終無(wú)明顯差異,即骨小梁BV/TV、Tb. N、Conn. D、BMD下降,Tb.Sp升高;Micro-CT重建顯示術(shù)后12周OVX組骨小梁較Sham組稀疏,缺失嚴(yán)重;HE染色顯示術(shù)后12周OVX組大鼠脛骨干骺端骨骺板下骨小梁變細(xì)且排列稀疏。結(jié)論OVX術(shù)骨質(zhì)疏松模型建立過(guò)程中大鼠成骨及破骨細(xì)胞活躍度先增高后降低,破骨細(xì)胞相對(duì)而言上升周期更長(zhǎng)且下降后能維持在更高水平,比成骨細(xì)胞更活躍,骨吸收大于骨生成導(dǎo)致骨質(zhì)疏松,組織學(xué)檢測(cè)結(jié)果表明骨小梁結(jié)構(gòu)不成熟。
[Abstract]:Objective to observe the dynamic changes of serum osteoconversion markers and bone trabecular structure at different time points in bilateral ovariectomized OVX SD rats. Methods 32 6-month-old female SD rats were randomly divided into sham-operated group and bilateral ovariectomized group (OVX) group. Samples were randomly selected at 4 time points after operation. Four animals were killed at each time point in each group. Serum levels of estradiol E2P, bone alkaline phosphatase (BALPN), tartrate-resistant acid phosphatase (-5b) and TRACP-5b were measured. The microstructures of bone trabeculae were observed by microCT Micro-CTand HE staining in invasive operation. Results E2 in OVX group was lower than that in Sham group 2 weeks after operation. At the 4th week to 12th week, there was a significant difference (P 0.05), while the level of E 2 in Sham group showed a decreasing trend after reaching the peak value 2 weeks after operation, and decreased to the preoperative level at the 12th week. However, the level of serum TRACP-5B in OVX group increased rapidly to the peak at the 4th week after operation, and then decreased, but at 12 weeks after operation, it was higher than that in Sham group and preoperative level. Micro-CT was used to analyze the relative bone volume (BV / TVV), the number of trabeculae and bone trabeculae in the metaphyseal region of the tibia (BV / TVV), and the trabeculae separation degree Tb.Spn.Conn.D, bone density (BMD) were not significantly different from those in the Sham group at 2 weeks, but there were significant differences at the beginning of the 4th week and at the 12th week, the difference was even more obvious. There was no significant difference in bone trabecular thickness and Tb. Th. the trabecular bone trabeculae (BV / TVV Tb.NbConn.DUBMD) decreased and Tb.Sp was increased. Micro-CT reconstruction showed that trabecular bone in OVX group was sparse than that in Sham group 12 weeks after operation. The results of HE staining showed that the trabeculae of the tibial metaphyseal epiphyseal plate of the OVX group became thin and sparse 12 weeks after operation. Conclusion the osteogenesis and osteoclast activity of the osteoblasts and osteoclasts increased first and then decreased during the establishment of the osteoporosis model of OVX. Osteoclasts had a longer ascending cycle and decreased osteoclasts, which were more active than osteoblasts, and bone resorption was larger than osteogenesis, resulting in osteoporosis. Histological examination showed that the trabecular structure of bone was immature.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R713.6

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