本文選題：卵泡刺激素受體　切入點：基因多態(tài)性　出處：《青島大學》2014年碩士論文

【摘要】：目的：探討卵泡刺激索受體(follicle stimulating hormone receptor, FSHR)基因第10外顯子680位點多態(tài)性與體外受精-胚胎移植(in vitro fertilization-embryo transfer, IVF-ET)患者卵巢反應性的關系。 方法：本研究為單中心的前瞻性癇例對照研究。將IVF-ET患者102例,按卵巢反應性分為3組,其中22例為卵巢低反應組,58例為正常卵巢組,22例為高反應組。采用聚合酶鏈反應(polymerase chain reaction, PCR)技術,檢測(FSHR)基因第10外顯子680位點多態(tài)性,比較三組患者的年齡、體重指數(body mass index, BMI)、不孕原因、基礎卵泡刺激素(basic FSH, bFSH)、竇卵泡數(antral follicle count, AFC)、人絨毛膜促性腺激素(human chorionic gonadotropin, HCG)注射日雌二醇(estradiol,E2)促性腺激素(gonadotropin, Gn)總用量、Gn使用天數和獲卵數。 結果：1.低反應組的Gn天數和Gn用量顯著多于正常反應組和高反應組,而高反應組的HCG日E2和獲卵數則多于低反應組和正常反應組,差異有統(tǒng)計學意義(P0.05)。 2.高反應組和正常反應組中AA型占比例最大(分別為54.5%,50.0%)而低反應組中SS型占比例最大(50.0%) 3.SS型的Gn用量明顯多于AA型和AS型(41.14±13.14),而獲卵數顯著低于其它兩型(8.64+5.39),差異有統(tǒng)計學意義(P0.05)。 結論：IVF-ET周期中卵巢反應性與FSHR基因第10外顯子680位點多態(tài)性有關。因此可以檢測FSHR基因的多態(tài)性來預測卵巢的反應性,指導臨床用藥,減輕患者的經濟和精神負擔。
[Abstract]:Objective: to investigate the relationship between the polymorphism at exon 6 80 of follicle stimulating hormone receptor (FSHR) gene and ovarian reactivity in patients with in vitro fertilization and embryo transfer in vitro fertilization-embryo transfer (IVF-ETS). Methods: one hundred and two patients with IVF-ET were divided into 3 groups according to their ovarian reactivity. Among them, 22 cases were ovarian hyporeactive group, 58 cases were normal ovary group, 22 cases were high response group. Polymerase chain reaction (PCR) technique was used to detect the polymorphism of exon 10 of the exon 10 of FSHR gene, and the age of the three groups was compared. Body mass index, mass, infertility, basal follicle stimulating hormone basic FSHs, bFSHS, antral follicle count, AFCU, human chorionic gonadotropin (HCG), estradiol estradioltropin (Gnn), the total dosage of gonadotropin (Gn) and the number of gonadotropin (Gn). Results 1. The days and dosage of Gn in the low reaction group were significantly more than those in the normal reaction group and the high reaction group, while in the high reaction group, the HCG day E 2 and the number of eggs obtained were more than those in the low reaction group and the normal reaction group. The difference was statistically significant (P 0.05). 2. The proportion of AA type in the high reaction group and the normal reaction group was the highest (54.5%), while the SS type in the low reaction group was 50.0%. The dosage of Gn in 3.SS type was significantly higher than that in AA type and as type (41.14 鹵13.14), and the number of eggs obtained was significantly lower than that of the other two types (P 0.05). Conclusion the ovarian reactivity in the IVF-ET cycle is related to the polymorphism at exon 10 of the FSHR gene. Therefore, the polymorphism of the FSHR gene can be used to predict ovarian reactivity, to guide the clinical use of drugs and to relieve the economic and mental burden of the patients.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R714.8

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本文編號：1656308