本文選題：MRI　切入點(diǎn)：孕早期　出處：《浙江大學(xué)》2014年碩士論文

【摘要】：目的： 近年來(lái),胎兒磁共振成像(Fetal magnetic resonance imaging, FMRI)在產(chǎn)科領(lǐng)域發(fā)展較快,其用于胎兒宮內(nèi)成像相較于目前常用的超聲具有無(wú)以比擬的優(yōu)越性。但是妊娠時(shí)期MRI檢查的安全性一直存在爭(zhēng)議。目前沒(méi)有證據(jù)表明臨床使用MRI會(huì)對(duì)胎兒的發(fā)育、出生及成長(zhǎng)產(chǎn)生不良的影響,但動(dòng)物實(shí)驗(yàn)中一些研究卻觀察到了畸形的發(fā)生。所以盡管大多認(rèn)為MRI無(wú)電離輻射,但其潛在的、尚不清楚的生物學(xué)效應(yīng)對(duì)胎兒的影響目前還不確切。另一方面,孕前3個(gè)月由于胚胎處于細(xì)胞分化發(fā)育期,容易受外界各種物理因素的損傷,這一時(shí)期胚胎異常發(fā)生率很高。但妊娠早期MRI對(duì)胎兒的副作用至今無(wú)相關(guān)研究,MRI生殖毒性的特征及其機(jī)制尚不清楚。蛋白質(zhì)組學(xué)是近年來(lái)發(fā)展起來(lái)的高通量篩查技術(shù),主要在整體水平研究機(jī)體蛋白質(zhì)的組成及其活動(dòng)規(guī)律。它為從全局的角度,動(dòng)態(tài)、整體研究胎兒MRI對(duì)孕早期胚胎命活動(dòng)中復(fù)雜的蛋白質(zhì)功能的影響提供了一種可能的高效手段,為胎兒MRI安全性探索提供了另一種全新的視角與途徑。 本課題擬以孕早期人滋養(yǎng)細(xì)胞Swan71為研究對(duì)象,蛋白質(zhì)組學(xué)為切入點(diǎn),研究臨床常用的1.5T胎兒MRI長(zhǎng)時(shí)間工作的情況下的潛在影響。 方法： 1.將人滋養(yǎng)細(xì)胞Swan71細(xì)胞在1.5T MRI常規(guī)工作條件下暴露60min后,采用WST-1檢測(cè)細(xì)胞增殖、流式細(xì)胞術(shù)檢測(cè)細(xì)胞周期,評(píng)估MRI暴露對(duì)孕早期人滋養(yǎng)細(xì)胞Swan71細(xì)胞功能的影響。 2.將人滋養(yǎng)細(xì)胞Swan71細(xì)胞在1.5TMRI常規(guī)工作條件下暴露60min后,采用透射電鏡技術(shù)觀察觀察細(xì)胞亞顯微結(jié)構(gòu)變化研究MRI對(duì)人滋養(yǎng)細(xì)胞Swan71的損傷作用； 3.將人滋養(yǎng)細(xì)胞Swan71細(xì)胞在1.5TMRI常規(guī)工作條件下暴露60min后,通過(guò)iTRAQ技術(shù)分析暴露組與對(duì)照組蛋白表達(dá)譜出現(xiàn)的差異,并初步分析這些蛋白質(zhì)的功能作用。 結(jié)果： 1.經(jīng)MRI暴露60分鐘后,人滋養(yǎng)細(xì)胞swan71細(xì)胞與對(duì)照組相比,增殖抑制明顯(p0.05),細(xì)胞周期無(wú)明顯改變。 2.電鏡觀察：暴露后線(xiàn)粒體腫脹明顯,體積變大變圓,基質(zhì)著色變淺、嵴變短變少。 3.用iTRAQ定量蛋白組學(xué)方法比較分析經(jīng)MRI暴露60分鐘和對(duì)照組之間人滋養(yǎng)細(xì)胞swan71細(xì)胞蛋白質(zhì)表達(dá)譜是否存在差異。最終發(fā)現(xiàn)79個(gè)差異蛋白,其中49個(gè)上調(diào),30個(gè)下調(diào),這些差異蛋白參與細(xì)胞生命及胚胎發(fā)育的重要方面。 結(jié)論： 1.60分鐘MRI暴露對(duì)孕早期人滋養(yǎng)細(xì)胞株swan71的功能與結(jié)構(gòu)可產(chǎn)生一定程度的影響。 2.MRI暴露可導(dǎo)致孕早期人滋養(yǎng)細(xì)胞株swan71蛋白質(zhì)表達(dá)譜發(fā)生改變。 3. ITRAQ蛋白質(zhì)譜分析是篩選MRI暴露相關(guān)差異蛋白表達(dá)的有效方法,為研究MRI對(duì)子代安全性研究提供新的視角。
[Abstract]:Objective:
In recent years, fetal magnetic resonance imaging (Fetal magnetic resonance imaging, FMRI) and rapid development in obstetrics, which is used for fetal imaging compared with the commonly used ultrasound has its incomparable superiority. But the safety inspection of the MRI period of pregnancy has been controversial. There is no evidence for clinical use of MRI for fetal development birth, and adverse effects on growth, but animal experiments in some studies have observed malformations. Although most of that MRI non ionizing radiation, but its potential is not clear, the biological effect of fetal impact is not exact. On the other hand, during the 3 months since the embryo in the cell the differentiation and development stage, various physical factors are susceptible to external damage, the embryo abnormal rate is very high. But the side effects of early pregnancy on fetal MRI has no related research, reproductive MRI The characteristics and mechanism of toxicity is unclear. Proteomics is a high-throughput screening technology developed in recent years, mainly in the study of the overall level of body protein composition and activities. It is from a global perspective, dynamic, overall impact of fetal MRI on protein function of complex life activities in early pregnancy embryo offers an efficient means possible, provides a new perspective and approach to explore the safety of fetal MRI.
The aim of this study is to take human gestational trophoblast Swan71 in early pregnancy as the research object. Proteomics is the breakthrough point to study the potential impact of 1.5T MRI on the long term work.
Method錛，

本文編號(hào)：1655192