發(fā)布時(shí)間：2018-03-21 16:20

  本文選題：子癇前期　切入點(diǎn)：JAR細(xì)胞　出處：《山東醫(yī)藥》2017年09期 　論文類型：期刊論文

【摘要】：目的探討miR-34a對人胎盤絨毛癌細(xì)胞JAR細(xì)胞自噬影響的機(jī)制。方法選取JAR細(xì)胞,隨機(jī)分為miR-34a模擬物組(轉(zhuǎn)染miR-34a模擬物)、NC組(轉(zhuǎn)染模擬物陰性對照)、miR-34a抑制物組(轉(zhuǎn)染miR-34a抑制物)、INC組(轉(zhuǎn)染抑制物陰性對照)、空白對照組(只加Lipofectamine 2000)、缺氧組(300μmol/L氯化鈷處理)和正常組(未經(jīng)氯化鈷處理)。采用Real time PCR法檢測各組miR-34a及HMGB1 mRNA表達(dá),Western blotting法檢測各組HMGB1和LC3Ⅱ/Ⅰ蛋白表達(dá),透射電鏡檢測各組細(xì)胞中自噬小體形成情況。結(jié)果 miR-34a模擬物組中HMGB1 mRNA和蛋白表達(dá)水平較NC組降低(P0.05),miR-34a抑制物組中HMGB1 mRNA和蛋白表達(dá)較INC組高(P0.05)。缺氧組中HMGB1和LC3Ⅱ/Ⅰ蛋白表達(dá)較正常組升高。缺氧處理后miR-34a模擬物組中HMGB1和LC3Ⅱ/Ⅰ蛋白的相對表達(dá)量較INC組降低(P0.05),miR-34a抑制物組中HMGB1和LC3Ⅱ/Ⅰ蛋白的相對表達(dá)量較INC組升高(P0.05)。透射電鏡結(jié)果顯示,缺氧組自噬小體的數(shù)量較正常組明顯增加(P0.05),缺氧處理后miR-34a模擬物組細(xì)胞中自噬小泡數(shù)量較NC組減少(P0.05),miR-34a抑制物組細(xì)胞中自噬小泡數(shù)量較INC組增加(P0.05)。結(jié)論 miR-34a可能通過抑制HMGB1表達(dá)影響滋養(yǎng)細(xì)胞的自噬。
[Abstract]:Objective to investigate the effect of miR-34a on autophagy of human placental choriocarcinoma JAR cells. Methods JAR cells were selected. The miR-34a mimics were randomly divided into two groups: the miR-34a mimic group (NC-transfected miR-34a mimic) group (miR-34a negative control group) (transfection of miR-34a inhibitor + INC group), the blank control group (Lipofectamine 2000 alone, hypoxia group containing 300 渭 mol/L Cobalt chloride). Real time PCR method was used to detect the expression of miR-34a and HMGB1 mRNA in each group. Western blotting method was used to detect the expression of HMGB1 and LC3 鈪，

本文編號：1644616