發(fā)布時間：2018-03-20 13:12

  本文選題：不明原因復(fù)發(fā)性流產(chǎn)　切入點：白細胞介素-18　出處：《浙江大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 探討人白細胞介素-18(IL-18)基因rs187238(-137G/C).rs360718(-119A/C)、 rs360717(-105G/A)位點多態(tài)性與不明原因復(fù)發(fā)性流產(chǎn)的關(guān)系。 方法 選取207例不明原因復(fù)發(fā)性流產(chǎn)患者和144例健康妊娠婦女進行研究。通過聚合酶鏈反應(yīng)(PCR)聯(lián)合DNA測序技術(shù)分析IL-18基因多態(tài)性。結(jié)果 不明原因復(fù)發(fā)性流產(chǎn)組rs187238(-137G/C)位點GG.GC+CC基因型分布頻率分別為77.3%、22.7%,與對照組GG.GC+CC基因型分布頻率(95.8%、4.2%)比較,差異有統(tǒng)計學(xué)意義(χ2=22.767,P=0.000)；不明原因復(fù)發(fā)性流產(chǎn)組rs187238(-137G/C)位點C等位基因頻率為13.04%,與對照組C等位基因頻率2.1%比較,差異亦有統(tǒng)計學(xué)意義(χ2=26.102,P=0.000)；等位基因頻率的相對風(fēng)險分析發(fā)現(xiàn),C等位基因攜帶者其自然流產(chǎn)的風(fēng)險是G等位基因攜帶者的7.050倍(OR=7.050CI：2.990～16.622)。rs360718和rs360717位點基因型分布與對照組均無統(tǒng)計學(xué)意義(χ2值分別為1.497和0.858,P值分別為0.221和0.354)；rs360718和rs360717位點兩組間等位基因分布頻率的差異均無統(tǒng)計學(xué)意義(P0.05)。 結(jié)論 IL-18基因啟動子rs187238(-137G/C)位點GC+CC基因型可能是復(fù)發(fā)性流產(chǎn)易感基因型,C等位基因可能是不明原因復(fù)發(fā)性流產(chǎn)發(fā)病的遺傳易感基因之一。IL-18基因外顯子rs360718、rs360717位點多態(tài)性與原因不明復(fù)發(fā)性流產(chǎn)可能無相關(guān)性。
[Abstract]:Purpose. To investigate the relationship between the polymorphism of rs187238- 137G / CU. Rs360718A- 119A / C, rs360717- 105G- 105G/ A) in human interleukin-18 (IL-18) gene and unexplained recurrent abortion. Method. The polymorphism of IL-18 gene was analyzed by polymerase chain reaction (PCR) and DNA sequencing in 207 patients with unexplained recurrent abortion and 144 healthy pregnant women. The distribution frequency of GG.GC CC genotype at rs187238- 137G / C) locus in recurrent abortion group with unknown cause was 77.3% and 22.7g, respectively, compared with that of control group (95.8%). The frequency of C allele at rs187238- 137G / C was 13.04g / C, which was higher than that of control group (2.1%), and the difference was statistically significant (蠂 ~ 2 / 22.767) (蠂 ~ (2 / 2) = 22.767), and the frequency of C allele was 13.04g / C in the group of recurrent abortion with unknown cause. The relative risk analysis of allelic frequency showed that the risk of spontaneous abortion was 7.050 times that of G allele carriers, and the genotype distribution of Rs360718 and rs360717 loci in G allele carriers was 7.050 times higher than that in G allele carriers. Neither the genotype distribution of Rs360718 nor that of the control group was normal. There was no significant difference in allele frequencies between the two groups (蠂 ~ 2 = 1.497 and 0.858g P = 0.221 and 0.354rs360718 and rs360717, respectively). Conclusion. IL-18 promoter rs187238-137G / C genotype GC CC genotype may be one of the genetic susceptibility genes for recurrent abortion with unknown cause. Polymorphism and causes of rs360718rs360717 gene exon rs360718rs360717 may be one of the genetic susceptibility genes of recurrent abortion with unknown cause. Unknown recurrent abortion may not be related.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R714.21

【參考文獻】

相關(guān)期刊論文 前4條

1 劉華鋒,陳孝文,吳平,江黎明,劉海燕,許勇芝,黃萍萍;狼瘡腎炎患者腎組織白細胞介素-18表達的研究[J];中華風(fēng)濕病學(xué)雜志;2002年02期

2 劉華鋒,陳孝文;白細胞介素-18與免疫性腎損傷[J];國外醫(yī)學(xué).泌尿系統(tǒng)分冊;2000年06期

3 陳佩;孫燕;蘇琳;岳錦春;;IFN-γ、IL-18與復(fù)發(fā)性流產(chǎn)的相關(guān)性研究[J];中國婦幼保健;2012年13期

4 劉爽;霍紅;;復(fù)發(fā)性自然流產(chǎn)的病因?qū)W研究進展[J];中國當(dāng)代醫(yī)藥;2013年02期

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本文編號：1639226