發(fā)布時(shí)間：2018-03-19 12:04

  本文選題：子宮內(nèi)膜癌　切入點(diǎn)：小腦鋅指結(jié)構(gòu)基因　出處：《中國(guó)腫瘤生物治療雜志》2017年08期 　論文類型：期刊論文

【摘要】：目的:探討小腦鋅指結(jié)構(gòu)1(zinc finger protein of the cerebellum 1,ZIC1)基因在人子宮內(nèi)膜癌組織中的表達(dá)情況及其與臨床參數(shù)之間的關(guān)系和預(yù)后預(yù)測(cè)價(jià)值。方法:收集江蘇大學(xué)附屬昆山醫(yī)院及上海市第六人民醫(yī)院自2008年1月至2011年12月收治的子宮內(nèi)膜癌患者43例,取腫瘤組織及相應(yīng)的距原發(fā)灶邊緣2 cm以上的癌旁黏膜組織標(biāo)本。應(yīng)用免疫組織化學(xué)法、Western blotting及RT-PCR法檢測(cè)43例子宮內(nèi)膜癌原發(fā)灶組織及癌旁黏膜組織中ZIC1的表達(dá),分析其與臨床病理參數(shù)之間的關(guān)系,并通過(guò)5年生存情況分析評(píng)價(jià)其預(yù)后價(jià)值。結(jié)果:子宮內(nèi)膜癌組織中ZIC1的m RNA水平和蛋白水平均明顯高于癌旁組織。ZIC1蛋白在子宮內(nèi)膜癌組織中呈現(xiàn)高表達(dá),其陽(yáng)性表達(dá)率為72.1%(31/43),在癌旁組織中陽(yáng)性表達(dá)率為39.5%(17/43)。ZIC1 m RNA的表達(dá)與淋巴結(jié)轉(zhuǎn)移及FIGO分期有關(guān)(P0.01或P0.05)。ZIC1低表達(dá)患者的5年生存率明顯高于高表達(dá)患者(66.7%vs 38.7%,P0.05)。單因素分析發(fā)現(xiàn)ZIC1(高表達(dá)vs低表達(dá))風(fēng)險(xiǎn)比(HR)為2.66,95%CI:1.07-5.89,P=0.047;經(jīng)多因素分析調(diào)整后,HR=2.25,95%CI:1.36-3.71,P=0.002,說(shuō)明高水平的ZIC1與子宮內(nèi)膜癌術(shù)后預(yù)后不良密切相關(guān)。結(jié)論:ZIC1表達(dá)水平的升高在子宮內(nèi)膜癌的發(fā)生發(fā)展中起著重要作用,可能成為子宮內(nèi)膜癌治療和預(yù)后測(cè)評(píng)的一個(gè)新的指標(biāo)。
[Abstract]:Objective: to investigate the expression of zinc finger finger protein of the cerebellum 1ZIC1 gene in human endometrial carcinoma and its relationship with clinical parameters and prognostic value. Methods: to collect Kunshan Hospital affiliated to Jiangsu University. And 43 cases of endometrial carcinoma treated in Shanghai 6th people's Hospital from January 2008 to December 2011. The expression of ZIC1 in 43 cases of endometrial carcinoma was detected by immunohistochemistry, Western blotting and RT-PCR were used to detect the expression of ZIC1 in 43 cases of endometrial carcinoma. The relationship between it and clinicopathological parameters was analyzed. Results: the level of m RNA and protein of ZIC1 in endometrial carcinoma was significantly higher than that in paracancerous tissue. The 5-year survival rate of patients with low expression of P0.01 or P0.05n / ZIC1 was significantly higher than that of patients with high expression of P0.01 or P0.05n. ZIC1 m RNA expression was significantly higher than that of patients with high expression of P0.01 or P0.05 / ZIC1. Univariate analysis showed that the expression of ZIC1 m RNA was significantly higher than that of patients with high expression of P0.01 or P0.05 / ZIC1. Univariate analysis showed that the expression of ZIC1 m RNA was significantly higher than that of patients with high expression of P0.01 or P0.05. The risk ratio of ZIC1 (high expression vs low expression) was 2.66% 95% CI: 1.07-5.89% P0. 047; adjusted by multivariate analysis, HR2. 25 95 CI: 1. 36-3. 71% P0. 002, indicating that high level of ZIC1 is closely related to poor prognosis after operation of endometrial carcinoma. Conclusion the increase of the expression level of VZIC1 in endometrial carcinoma is associated with the occurrence and development of endometrial carcinoma. Plays an important role, It may be a new indicator for the treatment and prognosis of endometrial carcinoma.
【作者單位】： 江蘇大學(xué)附屬昆山市第一人民醫(yī)院婦產(chǎn)科;江蘇大學(xué)附屬昆山市第一人民醫(yī)院普外科;上海市第六人民醫(yī)院婦科;
【基金】：江蘇大學(xué)醫(yī)學(xué)臨床科技發(fā)展基金資助項(xiàng)目(No.JLY20120050)~~
【分類號(hào)】：R737.33

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