本文選題：人乳頭瘤病毒　切入點：基因分型　出處：《中華腫瘤防治雜志》2017年11期 　論文類型：期刊論文

【摘要】：目的宮頸癌的發(fā)生發(fā)展與人乳頭瘤病毒(human papillomavirus,HPV)密切相關,本研究分析陜西省延安地區(qū)女性宮頸HPV感染情況及基因亞型年齡分布,為本地區(qū)女性宮頸癌防治和HPV感染的分子流行病學研究提供重要依據。方法選擇2011-01-01-2015-07-31延安大學附屬醫(yī)院體檢(478例)、"兩癌"篩查、機會性篩查(2 250例)及就診(814例)的3 542例女性患者作為研究對象,采用PCR體外擴增和DNA反向點雜交相結合的DNA芯片技術進行HPV基因分型檢測。結果 HPV感染率為40.54%(1 436/3 542),其中高危型HPV感染率38.42%(1 361/3 542);低危型HPV感染率3.78%(134/3 542);感染年齡分布以≥61歲年齡組感染率最高,其次是51~60歲年齡組。各年齡組HPV感染率和高危感染率比較差異均有統(tǒng)計學意義,P0.05;低危感染率比較差異無統(tǒng)計學意義,P0.05。按年齡組統(tǒng)計,高危亞型檢出頻次由高到低排列前3位,≤30歲年齡組為HPV16、HPV58和HPV39,其余各組均為HPV16、HPV58和HPV52。低危感染以HPV6和HPV11為主。單一型別感染占總感染人數的74.72%(1 073/1 436),其中單一高危感染占69.56%(999/1 436),單一低危占5.15%(74/1 436)。多重感染占總感染人數的25.27%(363/1 436),最高檢出四重感染。單一感染率、單一高危感染率最高的為41~50歲年齡組,多重高危感染率和高低�；旌隙嘀馗腥韭首罡叩氖恰�61歲年齡組,各年齡組高低危混合多重感染率比較差異有統(tǒng)計學意義(P0.05),其余感染類型差異均無統(tǒng)計學意義,P0.05。結論陜西省延安地區(qū)HPV感染年齡分布以≥61歲年齡組較高,主要亞型為HPV16、HPV58和HPV52,感染類型以單一感染為主,提示HPV亞型的分析對疫苗的研發(fā)和宮頸癌的篩查及防治有指導意義。
[Abstract]:Objective the occurrence and development of cervical cancer is closely related to human papillomavirus (HPV). This study analyzed the HPV infection and age distribution of cervical subtype in women in Yan'an area of Shaanxi Province. Methods 478 cases of female cervical cancer and HPV infection in Yanan University affiliated hospital were selected from 2011 to 01-2015-07-31, and "two carcinomas" were screened. 2 250 cases of opportunistic screening and 3 542 cases of female patients (814 cases) were studied. DNA microarray combined with PCR amplification in vitro and reverse dot hybridization of DNA was used to detect the genotyping of HPV. Results the infection rate of HPV was 40.54 and 1 436/3 542, of which the high risk HPV infection rate was 38.42%, the low risk HPV infection rate was 3.78% / 3 542%; the age of infection was 38.42%. The infection rate was highest in the age group 鈮，

本文編號：1626950