本文選題：羧甲基殼聚糖　切入點：載藥納米液滴　出處：《中國醫(yī)學影像技術》2017年06期 　論文類型：期刊論文

【摘要】：目的評價采用正己酰羧甲基殼聚糖制備相變型載阿霉素(DOX)納米液滴的體外超聲顯像效果及抑制卵巢癌細胞生長的作用。方法將羧甲基殼聚糖與正己酸酐發(fā)生�；磻铣烧乎ｔ燃谆鶜ぞ厶�,采用聲振乳化法制備正己酰羧甲基殼聚糖載藥納米液滴,表征其表面形態(tài)、粒徑、電位;評價其體外超聲顯像效果,以紫外分光光度計測定藥物包封率,采用CCK-8法檢測卵巢癌細胞存活率,并進行統(tǒng)計學分析。結果成功制備正己酰羧甲基殼聚糖載藥納米液滴,鏡下為大小均勻的球狀物,平均粒徑為(458.33±43.50)nm,包封率為(52.06±10.14)%。納米液滴可增強超聲顯影效果。超聲聯(lián)合載藥納米液滴組的卵巢癌細胞存活率[(62.54±3.60)%]低于DOX組[(75.55±7.21)%]、載藥納米液滴組[(76.18±4.94)%]和超聲組[(89.90±0.83)%],差異有統(tǒng)計學意義(P均0.05)。結論超聲聯(lián)合正己酰羧甲基殼聚糖載藥納米液滴可有效抑制卵巢癌細胞生長,在臨床診療中具有潛在應用價值。
[Abstract]:Objective to evaluate the effect of ultrasonic imaging of phase change doxorubicin (DOX) nanoparticles prepared by hexanoyl carboxymethyl chitosan on ovarian cancer cell growth. Methods Carboxymethyl chitosan was acylated with hexanoic anhydride. Synthesis of n-hexyl carboxymethyl chitosan by reaction, Nano-droplets loaded with n-hexyl carboxymethyl chitosan were prepared by acoustical emulsification method, their surface morphology, particle size and potential were characterized, the effect of ultrasonic imaging in vitro was evaluated, and the encapsulation efficiency was measured by ultraviolet spectrophotometer. CCK-8 assay was used to detect the survival rate of ovarian cancer cells, and the results showed that n-hexyl carboxymethyl chitosan nanoparticles were successfully prepared. The average particle size was 458.33 鹵43.50 nm, the encapsulation efficiency was 52.06 鹵10.140.The sonographic effect was enhanced by nano-droplets. The survival rate of ovarian cancer cells in the combination of ultrasonic and drug-loaded nano-droplet group [62.54 鹵3.60%] was lower than that in DOX group [75.55 鹵7.21%], the drug loaded nano-droplet group [76.18 鹵4.94 渭%] and the ultrasound group [89.90 鹵0.833%], the difference was statistically significant. Conclusion Ultrasound combined with n-hexanoyl carboxymethyl chitosan nanoparticles can effectively inhibit the growth of ovarian cancer cells. It has potential application value in clinical diagnosis and treatment.
【作者單位】： 廣州醫(yī)科大學附屬第三醫(yī)院藥學部;廣州醫(yī)科大學附屬第三醫(yī)院超聲醫(yī)學科;
【基金】：國家自然科學基金面上項目(81371572) 廣東省自然科學基金(2016A030311054) 廣州市科信局項目(201607010201)
【分類號】：R445.1;R737.31

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