本文選題：妊娠期肝內膽汁淤積癥　切入點：胎盤巨噬細胞　出處：《重慶醫(yī)科大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的:檢測ICP患者胎盤組織中TGR5和NF-κB的表達，初步探討TGR5和NF-κB在ICP中的作用。 方法:選取2013年3月-11月在重慶醫(yī)科大學附屬第一醫(yī)院產科行剖宮產的39例ICP孕婦為實驗對象，其中，，21例為ICP輕度組,18例為ICP重度組。對照組為同期行擇期剖宮產術的30例正常孕婦。應用免疫組化方法檢測各組孕婦胎盤組織TGR5和NF-κB的表達情況。 結果:(1)TGR5、NF-κB均表達于孕婦胎盤巨噬細胞及滋養(yǎng)細胞細胞膜和細胞質中。（2）在胎盤巨噬細胞中，ICP組TGR5表達強度低于對照組（Z=-4.237，P0.05)，ICP重度組TGR5表達強度低于輕度組（Z=-2.351，P0.05）；病例組NF-κB表達強度高于對照組（Z=-2.266，P0.05)；ICP重度組NF-κB表達強度高于輕度組（Z=-3.017，P0.05）。（3）在胎盤滋養(yǎng)細胞中，病例組TGR5表達強度低于對照組（Z=-2.397，P0.05)，ICP重度組TGR5表達強度低于輕度組（Z=-3.207，P0.05）；病例組NF-κB表達強度高于對照組（Z=-3.105，P0.05)；ICP重度組NF-κB表達強度高于輕度組（Z=-2.671，P0.05）。（4）TGR5和NF-κB在ICP組胎盤巨噬細胞中表達行相關性分析，呈負相關,相關系數分別為:-0.584，P值小于0.05；TGR5和NF-κB在ICP組胎盤滋養(yǎng)細胞中表達關系，也呈負相關,相關系數為:-0.424,P值小于0.05。 結論:ICP胎盤巨噬細胞和滋養(yǎng)細胞中TGR5表達下調，NF-κB表達上調，推測TGR5—NF-κB途徑可能參與ICP病理生理過程。
[Abstract]:Aim: to detect the expression of TGR5 and NF- 魏 B in placenta of patients with ICP and to explore the role of TGR5 and NF- 魏 B in ICP. Methods: from March 2013 to November, 39 pregnant women with ICP underwent cesarean section in the first affiliated Hospital of Chongqing Medical University. The expression of TGR5 and NF- 魏 B in placenta of 21 cases of mild ICP and 18 cases of severe ICP and 30 cases of normal pregnant women undergoing elective cesarean section at the same time were detected by immunohistochemical method. Results the TGR5 expression intensity of TGR5 in placental macrophages and trophoblast cell membrane and cytoplasm of pregnant women was lower than that in the control group (P 0.05, P 0.05), and the expression intensity of TGR5 in the severe group was lower than that in the mild group, and that in the case group was significantly lower than that in the mild group (P < 0.05), and the expression of TGR5- 魏 B in the placental macrophages was significantly lower than that in the control group (P < 0.05), while the expression of TGR5 in the placental macrophages was significantly lower than that in the control group (P 0.05). The expression of NF- 魏 B in the severe ICP group was higher than that in the mild group, and the expression of NF- 魏 B in the placental trophoblastic cells was higher than that in the mild group. The expression intensity of TGR5 in the severe group was lower than that in the mild group. The expression of NF- 魏 B in the severe group was higher than that in the control group. The expression intensity of NF- 魏 B in the severe group was higher than that in the mild group. The correlation between NF- 魏 B and NF- 魏 B in the placental macrophages of the ICP group was also analyzed, and the correlation between the expression of NF- 魏 B and the expression of NF- 魏 B in the placental macrophages of the mild group was also analyzed, and the correlation between the expression of NF- 魏 B and the expression of NF- 魏 B in the placental macrophages of the mild group was also analyzed, and the correlation between the expression of NF- 魏 B and the expression of NF- 魏 B in the placental macrophages was analyzed. There was a negative correlation between the expression of TGR5 and NF- 魏 B in placental trophoblastic cells in ICP group, and the correlation coefficient was: 1 / -0. 584 (P < 0. 05). Conclusion the down-regulation of TGR5 expression in ICP placental macrophages and trophoblastic cells suggests that TGR5-NF- 魏 B pathway may be involved in the pathophysiological process of ICP.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R714.25

【參考文獻】

相關期刊論文 前5條

1 秦朗,劉淑蕓,邢愛耘,蔡美英,趙忠蓉;胎兒淋巴細胞在妊娠期肝內膽汁淤積癥中的作用[J];華西醫(yī)學;2005年03期

2 彭冰,劉淑蕓;輔助性T淋巴細胞亞型與妊娠期肝內膽汁淤積癥發(fā)病的關系[J];中華婦產科雜志;2002年09期

3 王冬梅,朱啟英,丁麗,馬東;妊娠肝內膽汁淤積癥患者胎盤細胞凋亡及調控基因的研究[J];中華婦產科雜志;2003年01期

4 艾瑛,劉淑蕓;轉化生長因子β2對妊娠肝內膽汁淤積癥患者胎盤組織分泌腫瘤壞死因子α及γ干擾素的影響[J];中華婦產科雜志;2004年05期

5 Heather Francis;Shannon Glaser;Gianfranco Alpini;Gene LeSage;;Bile acid interactions with cholangiocytes[J];World Journal of Gastroenterology;2006年22期

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