本文選題：宮頸癌　切入點：PIAS-　出處：《實用臨床醫(yī)藥雜志》2014年16期 　論文類型：期刊論文

【摘要】：目的構(gòu)建PIAS-1 siRNA干擾載體Plko.1、PIAS-1。方法設(shè)計以PIAS-1基因為靶標(biāo)的siRNA序列,并克隆人載體Plko.1,用基因測序進行重組克隆驗證。結(jié)果 PCR檢測證實siRNA插入Plko.1質(zhì)粒,測序分析證實插入序列正確。結(jié)論成功構(gòu)建PIAS-1基因的siRNA載體,為研究PIAS-1基因?qū)m頸癌細(xì)胞增殖凋亡的影響提供了穩(wěn)定轉(zhuǎn)染的骨架載體。
[Abstract]:Objective to construct PIAS-1 siRNA interference vector Plko.1PIAS-1.Methods A siRNA sequence targeting PIAS-1 was designed and cloned into human vector Plko.1.Recombinant cloning was performed by gene sequencing. Results PCR analysis confirmed that siRNA was inserted into Plko.1 plasmid. Conclusion the siRNA vector of PIAS-1 gene was constructed successfully, which provides a stable transfection skeleton vector for studying the effect of PIAS-1 gene on the proliferation and apoptosis of cervical cancer cells.
【作者單位】： 蘭州大學(xué)第一臨床學(xué)院婦科;甘肅省人民醫(yī)院婦科;
【基金】：國家自然科學(xué)基金(81260387)
【分類號】：R737.33;Q78
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本文編號：1599839