本文選題：多囊卵巢綜合征　切入點(diǎn)：大鼠　出處：《四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2015年02期 　論文類型：期刊論文

【摘要】：目的應(yīng)用來曲唑建立多囊卵巢(PCO)大鼠模型,從組織及細(xì)胞水平測定模型大鼠卵巢氧化應(yīng)激狀態(tài),探討卵巢氧化應(yīng)激在多囊卵巢綜合征(PCOS)發(fā)病中的作用,為PCOS治療提供新的思路。方法將6周齡清潔級(jí)雌性SD大鼠,隨機(jī)編為實(shí)驗(yàn)組〔45只,予1%羧甲基纖維素溶液1mL/d+來曲唑1mg/(kg·d)灌胃〕和對(duì)照組(45只,僅予1%羧甲基纖維素溶液1mL/d灌胃),均持續(xù)28d。每日定時(shí)行陰道細(xì)胞涂片巴氏染色鏡檢,判斷動(dòng)情周期,每7d測量體質(zhì)量了解生長情況,第29d兩組大鼠統(tǒng)一處死、采血。測量指標(biāo):血清雌二醇(E2)、孕酮(P)、促卵泡刺激素(FSH)、促黃體生成素(LH)、睪酮(T)、性激素結(jié)合蛋白(SHBG),計(jì)算游離雄激素指數(shù)(FAI);解剖子宮、卵巢,稱重后計(jì)算器官質(zhì)量指數(shù);所得兩側(cè)卵巢,一側(cè)固定后石蠟切片HE染色,另一側(cè)制備組織勻漿、單細(xì)胞懸液,測定組織勻漿總氧化態(tài)(TOS)、總抗氧化態(tài)(TAS)、脂質(zhì)過氧化物丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力;檢測卵巢單細(xì)胞懸液細(xì)胞內(nèi)活性氧(ROS)水平。通過比較兩組大鼠上述指標(biāo)的差異,驗(yàn)證造模是否成功,分析卵巢組織氧化應(yīng)激水平與PCOS的關(guān)系。結(jié)果 1實(shí)驗(yàn)組用藥12~15d后動(dòng)情周期消失,體質(zhì)量增長明顯超過對(duì)照組(P0.05);2實(shí)驗(yàn)組性激素改變符合人類PCOS特征;3實(shí)驗(yàn)組卵巢質(zhì)量、卵巢指數(shù)大于對(duì)照組(P0.05),子宮質(zhì)量、子宮指數(shù)小于對(duì)照組(P0.05);4相對(duì)對(duì)照組大鼠,實(shí)驗(yàn)組大鼠卵巢HE切片鏡下表現(xiàn)為卵泡數(shù)量增多、白膜增厚、顆粒細(xì)胞層變薄、間質(zhì)增生等改變;5實(shí)驗(yàn)組大鼠卵巢組織內(nèi)MDA含量、TOS、氧化應(yīng)激指數(shù)(OSI)高于對(duì)照組,SOD活力、TAS低于對(duì)照組(P0.05);細(xì)胞內(nèi)ROS水平高于對(duì)照組(P均0.05)。結(jié)論1應(yīng)用來曲唑可成功誘導(dǎo)大鼠PCO模型,適于研究卵巢病變。2本方法所制備的PCO模型卵巢處于明顯的氧化應(yīng)激狀態(tài),存在細(xì)胞氧化損傷,推測人類PCOS卵巢組織內(nèi)可能也存在氧化應(yīng)激,因此對(duì)于PCOS的處理,在常規(guī)藥物治療同時(shí),應(yīng)注重抗氧化治療。
[Abstract]:Objective to study the role of ovarian oxidative stress in the pathogenesis of polycystic ovary syndrome (PCOS) in rats with polycystic ovary syndrome (PCOS) by using trazole to establish the model of PCO-induced ovarian oxidative stress in the tissue and cell levels of the model rats, and to explore the role of oxidative stress in the pathogenesis of polycystic ovary syndrome (PCOS). Methods six weeks old female SD rats of clean grade were randomly divided into experimental group (n = 45) and control group (n = 45) treated with 1% mL / d letrozole (1 mL / d) and control group (n = 45). Only 1% mL / d of carboxymethyl cellulose solution was given intragastrically for 28 days. The vaginal cell smears were examined by Pap staining every day to judge the estrous cycle, and the body mass was measured every 7 days to find out the growth of the rats. The rats in the two groups were killed at the same time on the 29th day. Measurements: serum estradiol estradiol, progesterone, follicle stimulating hormone (FSH), luteinizing hormone (LHG), testosterone (T), sex hormone binding protein (SHBG), free androgen index (FAI), anatomic uterus, ovary, organ mass index (OMI) after weighing. The tissue homogenate and single cell suspension were prepared on the other side. The total oxidative state of tissue homogenate, TASN, malondialdehyde (MDA) content, and the activity of superoxide dismutase (SOD) were measured in the tissue homogenate, the total oxidation state, the content of lipid peroxide malondialdehyde (MDA) and the activity of superoxide dismutase (SOD). The levels of reactive oxygen species (Ros) in ovarian single cell suspension cells were measured. By comparing the above indexes between the two groups of rats, it was verified whether the model was successful or not. Results 1 the oestrous cycle disappeared in the experimental group after 12 days of administration, and the body mass increased significantly than that in the control group (P 0.05). The changes of sex hormone in the experimental group were in line with the characteristics of human PCOS and the ovarian weight of the experimental group was in line with the characteristics of human PCOS. The ovarian index was higher than that of the control group (P 0.05), and the uterine weight was lower than that of the control group (P 0.05). The number of follicles, the thickness of the white membrane and the layer of granulosa cells were increased in the experimental group. The contents of MDA and oxidative stress index in ovarian tissue of experimental group were higher than that of control group (P 0.05), and the level of intracellular ROS was higher than that of control group (P 0.05). Conclusion 1 Trazole can be used to induce rat PCO model successfully. The PCO model, which is suitable for the study of ovarian pathological changes, is in a state of obvious oxidative stress and has cell oxidative damage. It is speculated that oxidative stress may also exist in the ovary of human PCOS. Therefore, the treatment of PCOS is related to the treatment of PCOS. At the same time, attention should be paid to antioxidation therapy.
【作者單位】： 四川大學(xué)華西第二醫(yī)院生殖內(nèi)分泌科;四川大學(xué)華西醫(yī)院感染性疾病中心;四川大學(xué)華西醫(yī)院呼吸內(nèi)科;四川大學(xué)華西第二醫(yī)院檢驗(yàn)科;四川大學(xué)華西公共衛(wèi)生學(xué)院衛(wèi)生統(tǒng)計(jì)學(xué)教研室;
【基金】：成都市科技局人口健康項(xiàng)目(No.12PPYD070SF-002)資助
【分類號(hào)】：R711.75;R-332

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