本文選題：人乳頭瘤狀病毒　切入點(diǎn)：L1殼蛋白　出處：《石河子大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：本研究通過檢測新疆漢族、維吾爾族婦女不同HPV亞型宮頸癌前病變中L1蛋白的表達(dá)情況，了解新疆漢族、維吾爾族婦女宮頸癌前病變中不同HPV亞型L1蛋白的表達(dá)差異。 方法：收集2012年9月至2013年6月新疆自治區(qū)人民醫(yī)院宮頸活檢結(jié)果為宮頸炎、CINI、CINII-III的HPVDNAHC2陽性的宮頸分泌物標(biāo)本共248例，其中漢族、維吾爾族宮頸炎各48例、46例，，CINI各40例、30例，CINII-III各28例、56例。采用堿裂解法提取HPVDNA，使用潮州凱普人乳頭狀瘤病毒核酸擴(kuò)增分型檢測試劑盒對提取出的HPVDNA進(jìn)行聚合酶鏈反應(yīng)（Polymerasechainreaction，PCR）擴(kuò)增和導(dǎo)流雜交方法檢測HPV基因型，石蠟組織切片經(jīng)二甲苯及梯度酒精脫蠟至水，后續(xù)具體操作按HPVL1檢測試劑盒（美國愛迪旺斯）免疫組化法說明書進(jìn)行檢測HPVL1蛋白。結(jié)果：（1）在248例宮頸癌前病變標(biāo)本中共檢測出13種高危HPV亞型（HPV16，58，52，53，68，31，66，18，39，33，35，56，59），2種低危HPV亞型（HPV6，81），其中HPV16型在維吾爾族、漢族中所占比例最高，分別為68.97%和65.15%，其次為HPV58、HPV52、HPV53型，百分比分別為32.76%、27%、27%，24.14%、15.5%，17.24%、15.5%。而HPV18型較少。 （2）在同一級別宮頸病變中漢族、維吾爾族HPVL1蛋白陽性表達(dá)率差異無統(tǒng)計學(xué)意義（P0.05）。漢族患者中慢性宮頸炎、CINI、CINII-III的HPVL1蛋白陽性表達(dá)率分別為50%，60%，21.43%，宮頸炎與CINI的差異無統(tǒng)計學(xué)意義（P0.05），CINI與CINII-III的HPVL1蛋白陽性表達(dá)率差異有統(tǒng)計學(xué)意義（P0.05）。維吾爾族患者中慢性宮頸炎、CINI、CINII-III的HPVL1蛋白陽性表達(dá)率分別為34.78%，46.67%，17.86%，宮頸炎與CINI的差異無統(tǒng)計學(xué)意義（P0.05），CINI與CINII-III的HPVL1蛋白陽性表達(dá)率差異有統(tǒng)計學(xué)意義（P0.05）。 （3）維吾爾族HPV16、非HPV16高危亞型中HPVL1蛋白的表達(dá)率分別為23.26%(20/86)、43.48%(20/46)，差異有統(tǒng)計學(xué)意義（P=0.016），HPV16型中漢族與維吾爾族HPVL1蛋白的表達(dá)率分別為42.5%（34/80）、23.26%(20/86)，差異亦有統(tǒng)計學(xué)意義（P=0.008）。 結(jié)論：（1）新疆維吾爾族婦女子宮頸癌中HPV感染以HPV16感染率最高，其次為HPV58，而HPV18型較少，與我國其他地區(qū)以HPV16、18型為主有差異，這就為HPV疫苗在新疆維吾爾族聚集地區(qū)的開發(fā)提供一定的理論依據(jù)。 （2）隨著CIN程度的加重，HPVL1蛋白的表達(dá)越來越低，差異有統(tǒng)計學(xué)意義，說明宮頸損傷的程度越重，HPV晚期蛋白L1的表達(dá)率越低，L1蛋白可作為判斷HPV感染后病毒復(fù)制狀態(tài)的有效指標(biāo)。 （3）維吾爾族中HPV16型L1蛋白的表達(dá)顯著低于非HPV16高危亞型，HPV16感染率高可能是因?yàn)長1蛋白表達(dá)缺失，使機(jī)體無法產(chǎn)生免疫反應(yīng)清除病變細(xì)胞，從而加劇了病變的進(jìn)展，引發(fā)宮頸癌； （4）HPV16型中維吾爾族HPVL1蛋白陽性表達(dá)率顯著低于漢族，提示HPVL1蛋白的表達(dá)缺失可能是維吾爾族宮頸病變患病率高于漢族的原因之一。
[Abstract]:Objective: to investigate the expression of L1 protein in different HPV subtypes of cervical cancer in Han and Uygur women in Xinjiang. Methods: from September 2012 to June 2013, a total of 248 cervical secretions were collected from cervical biopsies of Xinjiang Autonomous region people's Hospital. The cervical secretions were positive for CINII-III of CINII-III, including Han nationality. There were 48 cases of cervicitis in Uygur nationality and 46 cases of CINI each, and 30 cases of CINII-III and 56 cases of CINII-III respectively. HPVDNA was extracted by alkaline lysis method, and the extracted HPVDNA was detected by polymerase chain reaction using Kepu human papillomavirus nucleic acid amplification assay kit in Chaozhou. PCR amplification and diversion hybridization were used to detect HPV genotypes. Paraffin sections were dewaxed to water by xylene and gradient alcohol. The HPVL1 protein was detected according to the instructions of immunohistochemical method for HPVL1 detection kit (Adivence, USA). Results in 248 specimens of precancerous lesions, 13 high risk HPV subtypes were detected. HPV6 (HPV6) and HPV6 (HPV6), in which the HPV16 type is found in the Uygur ethnic group, The proportion of Han nationality was the highest, 68.97% and 65.15, respectively, followed by HPV58, HPV52, HPV53, and the percentages were 32.766.27 and 27.27, respectively. In the same grade of cervical lesions, the Han nationality, There was no significant difference in the positive expression rate of HPVL1 protein in Uygur patients (P 0.05). The positive expression rate of HPVL1 protein in chronic cervicitis of CINII-III was 50.60% and 21.433.There was no significant difference between cervicitis and CINI in HPVL1 protein expression of CINII-III and P0.05CINI. The positive expression rate of CINII-III in chronic cervicitis in Uygur patients was 34.78 ~ 46.67 and 17.86 respectively. There was no significant difference between cervicitis and CINI. There was significant difference in the positive expression rate of HPVL1 protein between CINI and CINII-III in Uygur nationality. (3) the expression rates of HPVL1 protein in HPV16 and non-HPV16 were 23.26 and 23.26, respectively. The difference was statistically significant between Han and Uygur. The expression rates of HPVL1 protein in HPV16 were 42.5%, 8023.26%, 2086%, respectively, and the difference was statistically significant. Conclusion in Xinjiang Uygur women, the infection rate of HPV16 is the highest, the next is HPV58, and the HPV18 type is less, which is different from other regions in China. This provides a theoretical basis for the development of HPV vaccine in Xinjiang Uygur gathering area. 2) with the severity of CIN, the expression of HPVL1 protein became lower and lower, and the difference was statistically significant. It indicated that the more serious the degree of cervical injury, the lower the expression rate of HPVL1 protein. The lower the expression rate of HPVL1 protein was, the lower the expression rate of HPVL1 protein could be as an effective index to judge the replication status of HPVL1 protein after HPV infection. (3) the high infection rate of HPV16 L1 protein in Uygur is significantly lower than that of non-#en1# high risk subtype HPV16, which may be due to the lack of L1 protein expression, which makes the body unable to produce immune response to clear the diseased cells, thus exacerbating the progression of the disease and causing cervical cancer. The positive expression rate of HPVL1 protein in Uygur nationality was significantly lower than that in Han nationality, suggesting that the loss of HPVL1 protein expression might be one of the reasons for the higher prevalence of cervical lesions in Uygur nationality than in Han nationality.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.33

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