本文選題：鐵缺乏　切入點：鐵轉運　出處：《中國現代醫(yī)學雜志》2017年20期 　論文類型：期刊論文

【摘要】：目的進一步確定二價金屬離子轉運體1(DMT1)在人胎盤絨毛膜癌細胞(Be Wo)中的表達定位,以及缺鐵狀態(tài)下m RNA的表達變化。方法采用免疫細胞化學技術觀察DMT1在Be Wo細胞中的表達定位,采用實時熒光定量聚合酶鏈反應檢測DMT1在Be Wo細胞缺鐵狀態(tài)下m RNA的表達變化。結果 DMT1在Be Wo細胞中呈陽性表達,其彌漫性表達定位于細胞質和細胞膜上;DMT1+IRE m RNA和DMT1-IRE m RNA的相對表達量隨缺鐵干預濃度和時間的增加而上調。DMT1+IRE m RNA的相對表達量高于DMT1-IRE m RNA。結論DMT1大量表達在Be Wo細胞質和細胞膜上,且表達受機體不同鐵水平的調節(jié),說明其在胎盤鐵轉運過程中發(fā)揮重要作用,為進一步探討胎盤鐵轉運分子機制提供實驗基礎。
[Abstract]:Objective to determine the expression localization of divalent metal ion transporter 1 (DMT1) in human placental choriocarcinoma cell line be Woe. Methods Immunocytochemical technique was used to observe the expression localization of DMT1 in be Wo cells. Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of m RNA in be Wo cells under iron deficiency. Results the expression of DMT1 was positive in be Wo cells. The diffuse expression of DMT1 IRE m RNA and DMT1-IRE m RNA in cytoplasm and cell membrane increased with the increase of iron-deficiency concentration and time. The relative expression of DMT1 IRE m RNA was higher than that of DMT1-IRE m RNA.Conclusion DMT1 is highly expressed in the cell membrane. Be Wo cytoplasm and cell membrane, The expression is regulated by different iron levels, which indicates that it plays an important role in the process of placental iron transport, which provides the experimental basis for further study of the molecular mechanism of placental iron transport.
【作者單位】： 陜西省西安市健康教育所;西安交通大學醫(yī)學部公共衛(wèi)生學院;
【基金】：國家自然科學基金(No:30800909)
【分類號】：R714
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本文編號：1570516