本文選題：妊娠滋養(yǎng)細胞腫瘤　切入點：葡萄胎　出處：《浙江大學》2014年碩士論文　論文類型：學位論文

【摘要】：背景 通過對家族性復發(fā)性葡萄胎的研究發(fā)現(xiàn)NLRP7基因是家族性復發(fā)性葡萄胎致病基因,可能通過炎癥通路導致其發(fā)生。葡萄胎是良性妊娠滋養(yǎng)細胞腫瘤,葡萄胎來源的妊娠滋養(yǎng)細胞腫瘤是否與NLRP7基因相關尚不確定。 研究目的 本研究通過對妊娠滋養(yǎng)細胞腫瘤患者和正常對照婦女的外周血淋巴細胞培養(yǎng),檢測其NLRP7蛋白,caspase-1蛋白,pro-IL-1β蛋白和IL-1β細胞因子的表達情況,間接檢測葡萄胎來源的妊娠滋養(yǎng)細胞腫瘤患者是否會引起功NLRP7蛋白功能障礙導致Casepase-1依賴的IL-1β因子的分泌異常。 材料與方法 選取10例病例組,10例對照組,其中病例組：10例GTT(先期妊娠為葡萄胎妊娠,且化療前抽血)對照組：10例正常人群(納入標準：沒有SLE等自身免疫性疾病的,沒有不良妊娠史的,沒有炎癥的人群)。分別對患者組和對照組經行LPS刺激,收集培養(yǎng)上液經行IL-1β因子測定,收集細胞采用Western Blotting方法測定NLRP7蛋白及caspase-1蛋白,pro-IL-1β蛋白表達情況,并對2組進行比較。 結果 (1)IL-1β細胞因子的分泌： 通過分析IL-1β細胞因子的分泌數(shù)據可得,LPS刺激后,病例組IL-1β細胞因子的分泌略低于對照組。2對病例差異明顯(標號11,,55)。 (2)NLRP7蛋白及caspase-1蛋白表達： 通過western blotting對6對NLRP7蛋白及caspase-1蛋白結果可得：2組NLRP7蛋白表達無差異,但其中2對病例48kd casepase-1蛋白在病例組無表達(標號11,,55)。 (3) pro-IL-1β蛋白表達： 通過western blotting2對pro-IL-1β蛋白表達結果可得：病例組pro-IL-1β蛋白表達略高于對照組。 結論： 葡萄胎來源的妊娠滋養(yǎng)細胞腫瘤48kd casepase-1在病例組不能正常表達,可能是casepase-1依賴的IL-1β細胞因子分泌低的可能原因之一,IL-1β細胞因子低分泌可能是葡萄胎來源的妊娠滋養(yǎng)細胞腫瘤的發(fā)病原因之一。
[Abstract]:Background. Through the study of familial recurrent hydatidiform mole, NLRP7 gene is found to be a gene causing familial recurrent hydatidiform mole, which may be caused by inflammation pathway. Hydatidiform mole is a benign gestational trophoblastic tumor. It is unclear whether gestational trophoblastic tumors derived from hydatidiform mole are associated with NLRP7 gene. Research purpose. In this study, the expression of NLRP7 protein caspase-1 protein pro-IL-1 尾 and IL-1 尾 cytokines in peripheral blood lymphocytes of patients with gestational trophoblastic tumor and normal women were detected. Indirect detection of gestational trophoblastic neoplasms from hydatidiform mole may lead to dysfunction of NLRP7 protein and abnormal secretion of Casepase-1 dependent IL-1 尾 factor. Materials and methods. Ten cases of control group were selected from 10 cases of case group, of which 10 cases in case group: 10 cases of GTT (gestation of hydatidiform mole before chemotherapy), 10 cases of normal population (inclusion standard: no autoimmune disease such as SLE, no history of adverse pregnancy), and 10 cases of normal population (inclusion standard: no autoimmune disease such as SLE, no history of adverse pregnancy). LPS stimulation was performed in the patients group and the control group, and the IL-1 尾 factor was measured in the culture supernatant. The expression of NLRP7 protein and caspase-1 protein pro-IL-1 尾 protein were measured by Western Blotting method, and the comparison was made between the two groups. Results. The secretion of IL-1 尾 cytokines:. By analyzing the secretion data of IL-1 尾 cytokines, it was found that the secretion of IL-1 尾 cytokines in the case group was slightly lower than that in the control group. The expression of NLRP7 protein and caspase-1 protein:. There was no difference in the expression of NLRP7 protein between the two groups by western blotting in 6 pairs of NLRP7 protein and caspase-1 protein, but no expression of casepase-1 protein on 48kd protein was found in 2 pairs of cases. 3) expression of pro-IL-1 尾 protein:. The expression of pro-IL-1 尾 protein in the case group was slightly higher than that in the control group through the expression of pro-IL-1 尾 protein by western blotting2. Conclusion:. Gestational trophoblastic tumors derived from hydatidiform mole were not expressed normally in 48 kd casepase-1. It may be one of the possible reasons for the low secretion of IL-1 尾 cytokines dependent on casepase-1. The low secretion of IL-1 尾 cytokines may be one of the causes of gestational trophoblastic tumors derived from hydatidiform mole.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.33

【參考文獻】

相關期刊論文 前2條

1 何浩明,曹傳蘊,田小平,徐鳳英,蘇彩女,莊惠琴;葡萄胎患者的紅細胞免疫功能研究[J];江西醫(yī)學檢驗;2000年04期

2 郭峰;紅細胞免疫及其調節(jié)功能測定方法[J];免疫學雜志;1990年01期

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