本文關(guān)鍵詞： 臍血干細(xì)胞移植 糖尿病 妊娠 胰島 血糖 胰島素 干細(xì)胞 臍帶臍血干細(xì)胞 妊娠期糖尿病 CD臍血干細(xì)胞 流式細(xì)胞分選技術(shù) 胰島素穩(wěn)態(tài)模型 空腹血糖 血脂　出處：《中國組織工程研究》2015年23期 　論文類型：期刊論文

【摘要】：背景:臍血中含有大量的造血干細(xì)胞、豐富的間充質(zhì)干細(xì)胞,與外周血及骨髓干細(xì)胞相比,新生兒臍血干細(xì)胞的異體排斥反應(yīng)小,免疫原性低。目的:觀察CD133臍血干細(xì)胞植入治療小鼠妊娠期糖尿病的可行性,為臨床妊娠期糖尿病的治療提供新的途徑。方法:采用高糖高脂喂養(yǎng)方法對(duì)孕鼠進(jìn)行妊娠期糖尿病造模,流式細(xì)胞分選技術(shù)對(duì)臍血干細(xì)胞進(jìn)行分離篩選。將篩選出的CD133臍血干細(xì)胞通過尾靜脈植入妊娠期糖尿病小鼠體內(nèi),移植7 d后,測定小鼠空腹血糖、空腹胰島素、總膽固醇和三酰甘油水平,采用蘇木精-伊紅染色觀察小鼠胰腺組織的損傷和修復(fù)情況,采用胰島素抵抗穩(wěn)態(tài)模型分析小鼠胰島素抵抗指數(shù)和胰島功能。結(jié)果與結(jié)論:采用流式細(xì)胞分選技術(shù)可以從臍血單核細(xì)胞中分選出CD133臍血干細(xì)胞,分選純度可達(dá)到(90.24±2.56)%;尾靜脈移植臍血干細(xì)胞7 d后,妊娠期糖尿病孕鼠總膽固醇、三酰甘油水平、空腹血糖和空腹胰島素水平均明顯下降(P0.05),胰島素抵抗指數(shù)下降,胰島功能改善(P0.05),孕鼠胰腺組織萎縮情況逆轉(zhuǎn),炎性浸潤減少。結(jié)果表明臍血干細(xì)胞移植可以使妊娠期糖尿病病情得到改善,可能與其修復(fù)胰腺損傷、降低胰島素抵抗指數(shù)、改善胰島功能有關(guān)。
[Abstract]:Background: umbilical cord blood contains a large number of hematopoietic stem cells and abundant mesenchymal stem cells. Compared with peripheral blood and bone marrow stem cells, the allograft rejection of umbilical cord blood stem cells in neonates is small. Objective: to observe the feasibility of CD133 umbilical cord blood stem cell implantation in the treatment of gestational diabetes mellitus in mice. To provide a new approach for the treatment of gestational diabetes mellitus (GDM). Methods: high glucose and high fat feeding method was used to model gestational diabetes mellitus in pregnant rats. Umbilical cord blood stem cells were isolated and screened by flow cytometry. The CD133 cord blood stem cells were implanted into gestational diabetic mice through tail vein. After 7 days of transplantation, fasting blood glucose and fasting insulin were measured in mice. The levels of total cholesterol and triacylglycerol were measured by hematoxylin-eosin staining to observe the damage and repair of pancreatic tissue in mice. The insulin resistance index and islet function of mice were analyzed by insulin resistance steady-state model. Results and conclusion: CD133 cord blood stem cells could be isolated from cord blood monocytes by flow cytometry. The total cholesterol, triglyceride, fasting blood glucose and fasting insulin levels in pregnant rats with gestational diabetes mellitus decreased significantly after transplantation of umbilical cord blood stem cells 7 days after transplantation of caudal vein. In pregnant rats, pancreatic tissue atrophy was reversed and inflammatory infiltration was decreased. The results showed that umbilical cord blood stem cell transplantation could improve the condition of gestational diabetes mellitus, which might be related to the repair of pancreatic injury and the decrease of insulin resistance index. Improvement of islet function.
【作者單位】： 重慶市開縣人民醫(yī)院產(chǎn)科;重慶醫(yī)科大學(xué);
【基金】：重慶市衛(wèi)生局醫(yī)學(xué)科研項(xiàng)目資助(CX201346)~~
【分類號(hào)】：R714.256

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本文編號(hào)：1517292