本文關(guān)鍵詞： 硫氧還蛋白結(jié)合蛋白 子癇前期 缺氧復(fù)氧　出處：《重慶醫(yī)科大學(xué)學(xué)報(bào)》2016年07期 　論文類型：期刊論文

【摘要】：目的:探討硫氧還蛋白結(jié)合蛋白(thioredoxin-interacting protein,TXNIP)激活核苷酸結(jié)合寡聚化結(jié)構(gòu)域樣受體蛋白3(NLR family pyrin domain containing 3,NLRP3)炎性小體與子癇前期(preeclampsia,PE)發(fā)病的關(guān)系。方法:收集重慶醫(yī)科大學(xué)附屬第一醫(yī)院2013年12月至2014年6月分娩的15例PE和15例正常產(chǎn)婦的胎盤(pán)組織,應(yīng)用Western blot檢測(cè)胎盤(pán)中TXNIP與NLRP3的表達(dá)水平。以人絨毛外滋養(yǎng)細(xì)胞(human transformed primary extravillous trophoblast cell line,HTR8/SVneo)體外培養(yǎng),分為正常對(duì)照組(N)組、缺氧復(fù)氧(hypoxia/reoxygenation,H/R)組、H/R+Scrambled si RNA(H/R+NC)組和H/R+TXNIP si RNA(H/R+si RNA)組。各組處理后使用Western blot檢測(cè)細(xì)胞TXNIP和NLRP3蛋白表達(dá)水平,應(yīng)用半胱氨酸蛋白酶-1(Caspase-1)活性檢測(cè)試劑盒檢測(cè)Caspase-1活性,應(yīng)用蛋白質(zhì)免疫共沉淀法(Co-Immunoprecipitation,Co-IP)檢測(cè)TXNIP與NLRP3、凋亡相關(guān)的斑點(diǎn)樣蛋白(apoptosis-associated speck-like protein containing CARD,ASC)之間的相互作用,并實(shí)時(shí)檢測(cè)細(xì)胞侵襲與增殖。結(jié)果:TXNIP與NLRP3在PE組胎盤(pán)組織中的表達(dá)明顯高于N組(P=0.003、P=0.002);在細(xì)胞模型中,H/R處理后,TXNIP與NLRP3的表達(dá)明顯升高(P=0.011、P=0.022),相互之間作用明顯加強(qiáng);抑制TXNIP后,NLRP3表達(dá)明顯降低(P=0.000),ASC蛋白水平也明顯下降(P=0.001),而且降低H/R對(duì)侵襲與增值能力的抑制作用(P=0.001、P=0.038)。結(jié)論:TXNIP介導(dǎo)的NLRP3炎性體激活可能在PE發(fā)病中起作用。
[Abstract]:Objective: to investigate thioredoxin-interacting protein. TXNP) activates nucleotide binding oligodeoxyribonucleotide domain like receptor protein 3 (NLR family pyrin domain containing 3). NLRP3) inflammatory corpuscles and preeclampsia. Methods: the placental tissues of 15 cases of PE and 15 cases of normal parturient were collected from December 2013 to June 2014 in the first affiliated Hospital of Chongqing Medical University. Western blot was used to detect the expression of TXNIP and NLRP3 in placenta. Human transformed primary extravillous trophoblast cell line. HTR 8 / SVneoa was cultured in vitro and divided into normal control group (n) and hypoxia reoxygenation / reoxygenation (H / R) group. H / R Scrambled si RNA(H/R si RNAs and H / R TXNIP si RNA(H/R siRNAs. After treatment, Western blot was used to detect the expression of TXNIP and NLRP3 protein. The activity of Caspase-1 was detected by cysteine protease-1 (Caspase-1) assay kit. TXNIP and NLRP3 were detected by Co-Immunoprecipitation Co-IP method. Apoptosis-associated speck-like protein containing CARD. Results the expression of NLRP3 and TXNIP in placenta of PE group was significantly higher than that of P0. 003 in group N. P0. 002; In the cell model, the expression of TXNIP and NLRP3 increased significantly after H- / R treatment, and the interaction between TXNIP and NLRP3 increased significantly. After inhibition of TXNIP, the expression of NLRP3 decreased significantly, and the level of ASC protein decreased significantly (P0. 001). Moreover, the inhibitory effect of H / R on invasion and value added ability was reduced by P0. 001. Conclusion the activation of NLRP3 inflammatory body mediated by TXNIP may play an important role in the pathogenesis of PE.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院婦產(chǎn)科重慶醫(yī)科大學(xué)中國(guó)-加拿大-新西蘭聯(lián)合母胎醫(yī)學(xué)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金面上資助項(xiàng)目(編號(hào):81370732)
【分類號(hào)】：R714.244
【正文快照】： 子癇前期(preeclampsia,PE)是妊娠期特有的疾病[1],而妊娠本就是一個(gè)炎癥過(guò)程,母胎界面的炎癥失衡是導(dǎo)致PE發(fā)生的原因之一[2]。有研究證明,PE患者血液中白介素-1β(interleukin-1β,IL-1β)水平高于正常,而IL-1β的分泌與核苷酸結(jié)合寡聚化結(jié)構(gòu)域樣受體蛋白3(NLR family pyrin

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 桑翠琴;張震宇;王淑珍;劉浩;郭淑麗;;重度子癇前期/子癇合并急性呼吸窘迫綜合征的診斷與處理[J];中國(guó)醫(yī)刊;2006年10期

2 趙桂花;;重度子癇前期與子癇82例臨床分析[J];臨床醫(yī)藥實(shí)踐雜志;2007年01期

3 楊小頎;嚴(yán)妮子;;子癇前期與血鈣水平相關(guān)性研究[J];實(shí)用臨床醫(yī)學(xué);2007年04期

4 楊小頎;嚴(yán)妮子;;子癇前期與血鈣水平相關(guān)性研究[J];四川生理科學(xué)雜志;2007年01期

5 陳茜;王澤華;;子癇前期的預(yù)測(cè)及預(yù)防[J];華中醫(yī)學(xué)雜志;2007年03期

6 朱倩;;對(duì)38例子癇前期(重)、子癇治愈的體會(huì)[J];實(shí)用預(yù)防醫(yī)學(xué);2007年05期

7 梁英;周麗麗;甘娟;姜碧洋;楊承東;;重度子癇前期在產(chǎn)科ICU監(jiān)護(hù)救治的臨床分析[J];中國(guó)婦幼保健;2008年19期

8 詹衛(wèi)星;鄭九生;;血管緊張素轉(zhuǎn)換酶基因多態(tài)性與重度子癇前期及腎功能損害的關(guān)系[J];江西醫(yī)藥;2008年08期

9 蔡穎;尹國(guó)武;姜鋒;李怡;閔保華;王s，

本文編號(hào)：1479597