本文關(guān)鍵詞： 妊娠高血壓綜合征 多態(tài)性 內(nèi)皮型一氧化氮合酶 限制性片段長度多態(tài)性聚合酶鏈反應(yīng) 孕婦　出處：《解剖學(xué)報(bào)》2017年03期 　論文類型：期刊論文

【摘要】：目的探討內(nèi)皮型一氧化氮合酶(eNOS)G894T基因多態(tài)性與青海省漢族妊娠高血壓綜合征(PIH)的相關(guān)性。方法采用限制性片段長度多態(tài)性聚合酶鏈反應(yīng)(PCR-RFLP)方法對138名妊娠高血壓綜合征患者和135名正常孕婦e NOS基因G894T多態(tài)性進(jìn)行分型,并測序驗(yàn)證。結(jié)果妊娠高血壓綜合征組基因型頻率野生型(GG)、雜合子型(GT)和突變純合子型(TT)分別為17.4%、82.6%、0;對照組分別為0、95.6%、4.4%,兩組基因型頻率分布有差異(P0.05)。妊娠高血壓綜合征組e NOS等位基因頻率G、T分別為58.7%、41.3%;對照組分別為47.8%、52.2%,兩組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),妊娠高血壓綜合征組G基因頻率高于對照組。結(jié)論e NOS基因G894T多態(tài)性可能與青海省漢族妊娠高血壓綜合征有關(guān),G等位基因可能為妊娠高血壓綜合征的易感基因(OR=1.229,95%CI:1.048~1.441),T等位基因可能為妊娠高血壓綜合征的保護(hù)基因。攜帶GG基因型的孕婦可作為青海省妊娠高血壓綜合征的易感人群。
[Abstract]:Objective to investigate the relationship between the polymorphisms of endothelial nitric oxide synthase (Enos) G894T gene and PIH in the Han nationality of Qinghai Province. Methods restriction fragment length polymorphism polymerase chain reaction (PCR RFLP) was used. Methods the polymorphisms of e NOS gene G894T in 138 patients with pregnancy-induced hypertension and 135 normal pregnant women were classified. Results the genotypes of GGG, GTT and TTT were 17.4% and 82.6% respectively in patients with pregnancy-induced hypertension syndrome (PIH). The genotype frequencies of the two groups were significantly different (P 0.05). The allele frequency of e NOS was G in the pregnancy induced hypertension syndrome group. T was 58.7 and 41.3 respectively; The control group was 47.8% and 52.2% respectively. The difference between the two groups was statistically significant (P 0.05). Conclusion the G894T polymorphism of e NOS gene may be associated with pregnancy-induced hypertension syndrome of Han nationality in Qinghai province. G allele may be the susceptibility gene of pregnancy induced hypertension syndrome. The T allele may be a protective gene for pregnancy-induced hypertension syndrome (PIH) and pregnant women with GG genotype can be used as susceptible populations of PIH in Qinghai province.
【作者單位】： 青海大學(xué)醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)部解剖學(xué)教研室;
【基金】：青海省科技廳計(jì)劃項(xiàng)目(2015-ZJ-746)
【分類號】：R714.246
【正文快照】： 妊娠高血壓綜合征(pregnancy-inducedhypertension syndrom,PIH)是產(chǎn)科常見疾病,是引起孕產(chǎn)婦及胎兒死亡的主要原因。國外發(fā)病率為7%~12%,我國為9.4%,高原地區(qū)為24%左右[1]。一氧化氮(NO)是重要的血管緊張性調(diào)節(jié)分子,在卵泡發(fā)育和成熟,胚胎植入,妊娠維持,分娩等許多生理過程中，

本文編號：1462119