本文關(guān)鍵詞： 卵巢癌 微小RNA 腹水 血清 免疫狀態(tài)　出處：《細(xì)胞與分子免疫學(xué)雜志》2015年03期 　論文類(lèi)型：期刊論文

【摘要】：目的觀察卵巢癌患者血清標(biāo)本中與樹(shù)突狀細(xì)胞分化發(fā)育調(diào)控有關(guān)的miRNA水平及其與臨床特征的關(guān)系,探討血清中miRNA的檢測(cè)在卵巢癌免疫狀態(tài)及預(yù)后判斷中的價(jià)值。方法采用實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)39例卵巢癌患者腹水和血清及20例健康女性成年人血清中miRNA的水平。結(jié)果卵巢癌患者血清中miR-21、miR-222和miR-142-3p水平顯著高于健康對(duì)照組,在臨床Ⅲ~Ⅳ期卵巢癌患者血清中miR-21水平高于Ⅰ~Ⅱ期,病理分級(jí)高的卵巢癌患者血清中miR-142-3p水平低于低級(jí)別的患者,而且患者腹水中miR-21和miR-222的水平高于外周血。結(jié)論卵巢癌患者血清和腹水miR-21、miR-222和miR-142-3p水平升高,與臨床分期病理分級(jí)有關(guān)。
[Abstract]:Objective to observe the level of miRNA and its relationship with clinical features in serum samples of ovarian cancer patients related to the regulation of dendritic cell differentiation and development. To evaluate the value of serum miRNA in the immune status and prognosis of ovarian cancer methods Real-time quantitative PCR technique was used to detect ascites and serum of 39 patients with ovarian cancer and 20 healthy women. Results the serum level of miR-21 in patients with ovarian cancer. The levels of miR-222 and miR-142-3p were significantly higher than those of healthy controls. The serum miR-21 levels in patients with stage 鈪，

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