本文關(guān)鍵詞： -羥維生素D EB 凋亡 侵襲　出處：《現(xiàn)代婦產(chǎn)科進展》2015年12期 　論文類型：期刊論文

【摘要】：目的:探討維生素D衍生物EB1089對宮頸癌細胞株凋亡及侵襲的影響。方法:采用酶聯(lián)免疫吸附試驗(ELISA)檢測45例宮頸炎癥、39例宮頸上皮內(nèi)瘤變(CIN)和44例宮頸鱗癌患者的血25-羥維生素D水平。流式細胞技術(shù)檢測細胞凋亡,Transwell試驗檢測細胞侵襲能力。結(jié)果:宮頸鱗癌組中血清25-羥維生素D水平顯著低于宮頸炎癥組和CIN組,差異有統(tǒng)計學(xué)意義(P0.05)。與0.1nmol/L EB1089組比較,10nmol/L EB1089組和100nmol/L EB1089組的宮頸癌細胞凋亡率均明顯增加,而細胞侵襲能力均顯著下降,呈顯著劑量依賴性。結(jié)論:維生素D衍生物EB1089抑制宮頸癌的發(fā)生發(fā)展,這為宮頸癌防治探索了新途徑。
[Abstract]:Objective: to investigate the effect of vitamin D derivative EB1089 on apoptosis and invasion of cervical cancer cell line. Methods: 45 cases of cervical inflammation were detected by Elisa. The blood levels of 25-hydroxyvitamin D in 39 patients with cervical intraepithelial neoplasia (cin) and 44 patients with cervical squamous cell carcinoma (SCC) were detected by flow cytometry. Results: serum 25-hydroxyvitamin D level in cervical squamous cell carcinoma group was significantly lower than that in cervix inflammation group and CIN group. The difference was statistically significant (P 0.05) compared with 0.1 nmol / L EB1089. The apoptosis rate of cervical cancer cells in 10nmol / L EB1089 group and 100nmol / L EB1089 group was significantly increased, while the cell invasion ability was significantly decreased. Conclusion: vitamin D derivative EB1089 inhibits the occurrence and development of cervical cancer, which provides a new approach for the prevention and treatment of cervical cancer.
【作者單位】： 南京市婦幼保健院檢驗科;
【基金】：國家自然科學(xué)基金面上項目(No:81272872) 南京市科技發(fā)展計劃項目(No:201308041)
【分類號】：R737.33
【正文快照】： Effect of vitamin D analogue EB1089 on apoptosis and invasion of human cervical cancercell.Wang Xiaoman,Gao Lingjuan,Gu Pingqing,et al.Department of Clinical Laboratory,Nan-jing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical Unive

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