本文關鍵詞：卵巢癌腫瘤間質中癌相關成纖維細胞全基因表達譜分析　出處：《重慶醫(yī)科大學學報》2017年11期 　論文類型：期刊論文

  更多相關文章： 上皮性卵巢癌 癌相關成纖維細胞 全基因表達譜 芯片

【摘要】：目的:尋找上皮性卵巢癌(epithelial ovarian cancer,EOC)癌相關成纖維細胞(cancer associated fibroblasts,CAFs)和正常成纖維細胞(normal fibroblasts,NFs)中差異表達的m RNA并進行分析,為進一步闡明腫瘤間質中癌相關成纖維細胞影響卵巢癌細胞惡性行為的機制提供依據(jù)。方法:分別提取3例上皮性卵巢癌的CAFs及3例正常卵巢組織的NFs,抽提總RNA,進行全基因表達譜分析。對差異基因進行基因本體論(Gene Ontology,GO)富集及信號通路聯(lián)合分析,推定相關差異基因生物學功能,并對MDM2、NR3C1等6個上調的差異基因進行熒光定量PCR驗證。結果:對芯片結果的分析顯示差異基因共1 636個。q RTPCR對MDM2、NR3C1等6個差異基因的驗證結果與芯片結果相符,生物信息學分析顯示上述差異基因與癌相關通路、肌動蛋白細胞骨架重塑通路等有關,提示可能參與了對卵巢癌細胞惡性行為的促進作用。結論:EOC腫瘤間質中的成纖維細胞存在調控癌細胞的差異基因,分析這些差異基因為進一步研究卵巢癌成纖維細胞調控癌細胞生物學行為的分子機制提供了基本依據(jù)。
[Abstract]:Objective: to search for epithelial ovarian cancer (epithelial ovarian, cancer, EOC) cancer associated fibroblasts (cancer associated, fibroblasts, CAFs) and normal fibroblasts (normal, fibroblasts, NFs) in the differential expression of M RNA and analysis, provides the basis for further clarify the tumor stroma in cancer related to the malignant behavior of ovarian cancer cells the mechanism of the influence of fiber cells. Methods: CAFs were extracted from 3 cases of epithelial ovarian cancer and 3 cases of normal ovarian tissue NFs, extract the total RNA spectrum to analyze the gene expression of different genes. Gene Ontology (Gene Ontology, GO) and signaling pathway enrichment analysis, correlation between the putative gene biological functions and, MDM2, fluorescence quantitative PCR validation of differential gene NR3C1 6 up-regulated. Results: analysis of the microarray results showed differences in 1636 genes of.Q RTPCR MDM2, gene NR3C1 6 differences The results are consistent with the microarray results, bioinformatics analysis showed that the difference of gene and cancer related pathways, actin cytoskeleton remodeling pathway, suggesting that it may be involved in the malignant behavior of ovarian cancer cells. Conclusion: EOC tumor interstitial fibroblast gene is differentially regulated in cancer cells, analysis the basis for these differential genes for further research of the molecular mechanism of ovarian cancer cells into fibroblasts regulates the biological behavior of cancer.

【作者單位】： 重慶醫(yī)科大學附屬第一醫(yī)院婦產(chǎn)科;
【基金】：國家自然科學基金面上資助項目(編號:81572562)
【分類號】：R737.31
【正文快照】： 上皮性卵巢癌(epithelial ovarian cancer,EOC)是目前死亡率最高的婦科惡性腫瘤。由于缺乏早期篩查手段,70%以上的患者診斷時已出現(xiàn)廣泛的盆腹腔轉移[1]。雖然給予了手術治療輔助放化療,復發(fā)率仍高達80%[2]。癌癥的進展過程不僅依賴于癌細胞本身的特性,更與腫瘤微環(huán)境的特性相，

本文編號：1438166