本文關鍵詞：體外受精患者精子miRNA-21,miRNA-34c,miRNA-140,miRNA-375與胚胎質量的相關性研究　出處：《中南大學學報(醫(yī)學版)》2015年08期 　論文類型：期刊論文

  更多相關文章： 微小RNA 精子 體外受精-胚胎移植 胚胎質量

【摘要】：目的:通過檢測不同胚胎質量的體外受精(in vitro fertilization,IVF)患者精子mi RNA-21,mi RNA-34c,mi RNA-140,mi RNA-375的表達情況,探討精子來源的mi RNAs與胚胎質量的相關性。方法:選擇2012年9月至12月在中南大學湘雅醫(yī)院生殖醫(yī)學中心行IVF治療原發(fā)不育的男性患者44例,收集IVF取卵當日剩余新鮮精液標本,采用實時熒光定量PCR測定精子mi RNAs(mi RNA-21,mi RNA-34c,mi RNA-140,mi RNA-375)的表達水平。觀察胚胎情況后分組,第3天胚胎評分的平均值8分為實驗組,≥8分為對照組。比較實驗組和對照組患者一般情況及實驗室資料,分析精子mi RNAs表達水平與胚胎質量的相關性。結果:實驗組精子mi RNA-21,mi RNA-34c,mi RNA-140,mi RNA-375表達水平低于對照組(P0.01)。實驗組與對照組的獲卵數(shù)、減數(shù)分裂II期(metaphase II,MII)卵子數(shù)、雙原核(dual pronuclear,2PN)受精數(shù)、卵裂數(shù)、受精率差異無統(tǒng)計學意義(P0.05);實驗組卵裂率低于對照組(P0.05)。精子mi RNA-21,mi RNA-34c,mi RNA-140,mi RNA-375表達水平與第2,3天胚胎碎片率呈負相關,與第3天胚胎卵裂球數(shù)呈正相關,與胚胎評分呈正相關。結論:精子mi RNA-21,mi RNA-34c,mi RNA-140,mi RNA-375的表達水平上升可能影響卵裂期胚胎質量,對胚胎發(fā)育可能起一定的積極作用。
[Abstract]:Objective: to detect the sperm mi RNA-21 of patients with in vitro fertilization vitro fertilization (IVF) with different embryo quality. The expression of miRNA-34cnmRNA-140 RNA-375. To investigate the correlation between sperm source mi RNAs and embryo quality. Methods:. From September 2012 to December, 44 male patients with primary infertility were treated with IVF in the Center of Reproductive Medicine, Xiangya Hospital, Central South University. The remaining fresh semen samples from IVF were collected on the same day. Real time fluorescence quantitative PCR was used to determine the sperm mi RNAs(mi RNA-21mmi RNA-34c. The expression level of mi RNA-140 mRNA-375) was observed and divided into groups. The average embryo score on the 3rd day was divided into experimental group (n = 8). 鈮，

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