本文關(guān)鍵詞：人子宮頸癌腫瘤相關(guān)巨噬細胞浸潤與T細胞亞型及臨床病理特征的相關(guān)性分析　出處：《基因組學(xué)與應(yīng)用生物學(xué)》2016年12期 　論文類型：期刊論文

  更多相關(guān)文章： 腫瘤相關(guān)巨噬細胞 宮頸癌 FOXP+Tregs CD+T 淋巴結(jié)轉(zhuǎn)移

【摘要】：為了探討腫瘤相關(guān)巨噬細胞(tumor associated macrophages,TAMs)在人子宮頸癌微環(huán)境內(nèi)的分布與T細胞亞型和臨床病理特征的相關(guān)性,本研究應(yīng)用S-P免疫組化法檢測TAMs在60例子宮頸癌組織、10例高級別鱗狀上皮內(nèi)病變組織及10例子宮頸組織的分布情況;間接免疫熒光雙標(biāo)法分析浸潤的FOXP3~+Treg細胞數(shù)量、CD8~+T細胞數(shù)量及其粒酶B分泌情況。統(tǒng)計學(xué)分析TAMs數(shù)量與癌間質(zhì)浸潤的FOXP3~+Treg、CD8~+T數(shù)量和臨床病理特征的相關(guān)性。結(jié)果顯示TAMs在子宮頸癌微環(huán)境的數(shù)量均顯著多于高級別鱗狀上皮內(nèi)病變和子宮頸組織(p0.01,p0.01),同時高級別鱗狀上皮內(nèi)病變組織陽性細胞數(shù)也明顯多于子宮頸組織(p0.01);TAMs數(shù)量與宮頸癌間質(zhì)內(nèi)浸潤的FOXP3~+T細胞數(shù)量呈正相關(guān)性(p0.05),且與淋巴結(jié)轉(zhuǎn)移和癌栓相關(guān)(p0.05,p0.05),但與CD8~+T細胞浸潤數(shù)量無明顯相關(guān)性(p0.05)。本實驗發(fā)現(xiàn)TAMs在宮頸癌微環(huán)境內(nèi)大量分布,且與腫瘤局部免疫抑制及淋巴結(jié)轉(zhuǎn)移和癌栓有關(guān)。
[Abstract]:In order to study tumor associated macrophage tumor associated macrophages. The distribution of Tams in the microenvironment of human cervical cancer was correlated with T cell subtypes and clinicopathological features. S-P immunohistochemical method was used to detect TAMs in 60 cases of cervical carcinoma. The distribution of 10 cases of high grade squamous intraepithelial lesions and 10 cases of cervical tissues. Indirect immunofluorescence double labeling method was used to analyze the number of FOXP3- Treg cells. The number of CD8 ~ T cells and the secretion of granulocyte B. the number of TAMs and FOXP3- Treg of interstitial invasion were analyzed statistically. The correlation between the number of CD8T and clinicopathological features. The results showed that the number of TAMs in the microenvironment of cervical cancer was significantly higher than that in high-grade squamous intraepithelial lesions and cervical tissue (p0.01). At the same time, the number of positive cells in high grade squamous intraepithelial lesions was significantly higher than that in cervical tissues. The number of TAMs was positively correlated with the number of FOXP3T cells infiltrated in the stroma of cervical carcinoma, and was correlated with lymph node metastasis and tumor thrombus. However, there was no significant correlation between the number of CD8T cells and the number of CD8T cells. In this study, we found that TAMs was widely distributed in the microenvironment of cervical cancer. And related to tumor local immunosuppression, lymph node metastasis and tumor thrombus.
【作者單位】： 重慶醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院病理教研室分子醫(yī)學(xué)與腫瘤研究中心;
【基金】：重慶市自然科學(xué)基金(CSTC2009BB5268)項目資助
【分類號】：R737.33
【正文快照】： *通訊作者,2402316007@qq.comCorrelation Analysis of Tumor Associated Macrophages and Subtype ofT-lymphocytes and Clinicopathological Features in Human Cervical CancerShen Yifan Xu Man*Wang Xin Wang Tao Liu HuiDepartment of Pathology,Molecular Medicine and

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