發(fā)布時(shí)間：2018-01-14 18:05

  本文關(guān)鍵詞：維生素C及其轉(zhuǎn)運(yùn)體SVCT在子癇前期發(fā)病機(jī)制中的作用研究　出處：《實(shí)用婦產(chǎn)科雜志》2016年04期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 子癇前期 維生素C 氧化應(yīng)激 依賴(lài)鈉離子維生素C轉(zhuǎn)運(yùn)蛋白

【摘要】：目的:通過(guò)前瞻性隨機(jī)對(duì)照臨床研究,探討圍生期維生素C的攝入及代謝與子癇前期(PE)的關(guān)系,探索PE的可能發(fā)病機(jī)制。方法:隨機(jī)選取2011年9月至2014年3月廣州醫(yī)科大學(xué)附屬第三醫(yī)院住院孕婦344例,包括研究組(PE患者)170例、對(duì)照組(正常妊娠)174例。記錄每日維生素C攝入量;收集其清晨中段尿、外周血、臍血及胎兒附屬物(胎盤(pán)、胎膜、臍帶),采用ELISA方法檢測(cè)母體尿液、外周血及臍血血清維生素C含量,免疫組化法觀察胎盤(pán)、胎膜、臍帶依賴(lài)鈉離子維生素C轉(zhuǎn)運(yùn)蛋白(SVCT)1及SVCT2的表達(dá)情況,PCR-RFLP法檢測(cè)SLC23a1的多態(tài)性。結(jié)果:1研究組每日維生素C攝入量及尿液維生素C含量與對(duì)照組相比,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);2研究組母體外周血維生素C含量明顯高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);3研究組臍血維生素C含量低于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);4研究組胎兒附屬物SVCT1及SVCT2表達(dá)水平均較對(duì)照組低,差異有統(tǒng)計(jì)學(xué)意義(P0.05);5研究組與對(duì)照組SLC23a1多態(tài)性在rs35817838、rs33972313、rs6886922和rs34521685位點(diǎn)上并無(wú)差異(P0.05)。結(jié)論:胎兒附屬物SVCT1及SVCT2表達(dá)水平降低,從而影響母體與胎兒之間的維生素C的轉(zhuǎn)運(yùn),使得維生素C等抗氧化物不能有效到達(dá)胎盤(pán),這可能是PE發(fā)病機(jī)制之一。
[Abstract]:Objective: to investigate the relationship between perinatal vitamin C intake and metabolism and preeclampsia (PEP) by prospective randomized controlled clinical study. Methods: from September 2011 to March 2014, 344 pregnant women in the third affiliated Hospital of Guangzhou Medical University were randomly selected. There were 170 patients with PE in the study group and 174 patients in the control group (normal pregnancy). Daily vitamin C intake was recorded. Urine, peripheral blood, umbilical cord blood and fetal appendages (placenta, fetal membrane, umbilical cord) were collected. Serum vitamin C levels in maternal urine, peripheral blood and umbilical cord blood were detected by ELISA method. The expression of SVCT-1 and SVCT2 in placenta, fetal membrane and umbilical cord dependent vitamin C transporter was observed by immunohistochemical method. Results the daily vitamin C intake and urine vitamin C content in the study group were compared with those in the control group. The difference was not statistically significant (P 0.05). 2the content of vitamin C in maternal peripheral blood of the study group was significantly higher than that of the control group, and the difference was statistically significant (P 0.05). 3The content of vitamin C in the study group was lower than that in the control group, and the difference was statistically significant (P 0.05). (4) the expression of SVCT1 and SVCT2 in the study group was lower than that in the control group (P 0.05). 5 the polymorphism of SLC23a1 in the study group and the control group was rs35817838 and rs33972313. There was no difference between rs6886922 and rs34521685 loci (P 0.05). Conclusion: the expression of SVCT1 and SVCT2 in fetal appendages is decreased. Thus affecting the transport of vitamin C between mother and fetus, vitamin C and other antioxidants can not reach the placenta effectively, which may be one of the pathogenesis of PE.
【作者單位】： 廣州醫(yī)科大學(xué)附屬第三醫(yī)院廣東省產(chǎn)科重大疾病重點(diǎn)實(shí)驗(yàn)室;
【基金】：廣東省自然科學(xué)基金(編號(hào):S2013010014810)
【分類(lèi)號(hào)】：R714.244
【正文快照】： 子癇前期(preeclampsia,PE)發(fā)病率為9.4%,是導(dǎo)致全球孕產(chǎn)婦和圍生兒發(fā)病及死亡的主要原因之一[1]。目前發(fā)病機(jī)制尚不明確,多數(shù)學(xué)者認(rèn)為,其起源于胎盤(pán)的病理生理改變,胎盤(pán)淺著床及隨之產(chǎn)生的氧化應(yīng)激可能是PE發(fā)病的首要因素,而滋養(yǎng)細(xì)胞侵襲能力下降以及子宮螺旋動(dòng)脈重鑄障礙是P

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