本文關鍵詞：囊胚內細胞團和滋養(yǎng)外胚層基因表達研究　出處：《中華生殖與避孕雜志》2017年09期 　論文類型：期刊論文

  更多相關文章： 囊胚 內細胞團 滋養(yǎng)外胚層 基因 單細胞測序

【摘要】：目的研究人胚胎植入前囊胚內細胞團和滋養(yǎng)外胚層細胞基因的表達。方法收集輔助生殖來源人受精后6 d Gardner評分5AA囊胚,顯微鏡下微吸管機械分離內細胞團和滋養(yǎng)外胚層細胞,單細胞測序檢測,選取篩選的差異表達基因并進行無監(jiān)督層次聚類分析和Gene Ontology(GO)功能分類分析。結果對3枚受精后6 d的5AA囊胚內細胞團14個單細胞和滋養(yǎng)外胚層19個單細胞測序,結果顯示內細胞團1 283個基因表達上調,其G O生物學功能主要是參與DNA依賴的轉錄、生物種間相互作用、抗原加工和表達、免疫反應、信號轉導、氧化還原、細胞分化、抗凋亡、神經系統(tǒng)發(fā)育、細胞黏附等,參與絲裂原活化蛋白激酶(MAPKs)信號通路、肌動蛋白細胞骨架、黏附、軸引導、Jak-STAT、Wnt信號通路。滋養(yǎng)外胚層1 073個基因表達上調,其GO生物學功能主要是參與轉錄、DNA依賴的轉錄、細胞周期、蛋白分解代謝、蛋白氨基酸磷酸化、蛋白質運輸、細胞分裂、有絲分裂、泛素依賴性蛋白分解、細胞內蛋白質轉運等;參與泛素介導蛋白水解、鞘脂代謝、纈氨酸,亮氨酸和異亮氨酸降解、果糖和甘露糖代謝、氨基磷酸酯代謝、類固醇的生物合成、抗原處理和表達等信號通路。結論從空間維度揭示囊胚內細胞團和滋養(yǎng)外胚層的基因表達,顯示兩者相互協(xié)調,精細調節(jié)囊胚發(fā)育和胚胎植入過程,進一步數(shù)據(jù)分析有望尋找到調控胚胎植入的內源性關鍵特異分子。
[Abstract]:Objective to study the gene expression of cell mass and trophodermal cell in preimplantation blastocyst. Methods 5AA blastocysts were collected on the 6th day after assisted reproduction. Micropipette mechanical isolation of inner cell mass and trophoblast ectodermal cells were examined by single cell sequencing. The differentially expressed genes were selected and analyzed by unsupervised hierarchical clustering and Gene Ontology (GOG). Results three 5AA blastocysts from 6 days after fertilization were sequenced by 14 single cells and 19 single cells in the trophodermal layer. The results showed that 1,283 genes were up-regulated in the inner cell mass, and their biological functions were mainly involved in DNA dependent transcription, interspecies interaction, antigen processing and expression, and immune response. Signal transduction, redox, cell differentiation, anti-apoptosis, nervous system development, cell adhesion, mitogen-activated protein kinase (MAPKs) signaling pathway, actin cytoskeleton, adhesion. The expression of 1073 genes was up-regulated in the trophodermal layer. Its go biological function was mainly involved in transcription of DNA dependent transcription and cell cycle. Protein catabolism, protein amino acid phosphorylation, protein transport, cell division, mitosis, ubiquitin dependent protein decomposition, intracellular protein transport, etc. Participate in ubiquitin mediated protein hydrolysis, sphingolipid metabolism, valine, leucine and isoleucine degradation, fructose and mannose metabolism, aminophosphate metabolism, steroid biosynthesis. Conclusion the gene expression in blastocyst and trophodermal layer is revealed from the spatial dimension, which shows that the two genes coordinate with each other and regulate the development of blastocyst and embryo implantation. Further data analysis is expected to find key endogenous specific molecules regulating embryo implantation.
【作者單位】： 北京積水潭醫(yī)院婦產科;
【基金】：國家自然科學基金面上項目(81070493)~~
【分類號】：R714.8
【正文快照】： 哺乳動物胚胎早期發(fā)育具有自我調控能力,胚胎沒有極化之前,改變卵裂球的空間位置,或者移除一個卵裂球,胚胎都能正常發(fā)育。胚胎逐漸分裂和發(fā)育,細胞開始具有極性和特異性,這種分化發(fā)育可以追溯到胚胎發(fā)育早期。對小鼠4-細胞胚胎研究證實[1],細胞具有發(fā)育特異性,細胞質成分差異，

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