本文關(guān)鍵詞：姜黃素對(duì)宮頸癌干細(xì)胞增殖、聚集及化療敏感性的影響　出處：《山東醫(yī)藥》2017年37期 　論文類型：期刊論文

  更多相關(guān)文章： 宮頸癌 宮頸癌干細(xì)胞 姜黃素 細(xì)胞增殖 細(xì)胞聚集 貝伐單抗 治療敏感性

【摘要】：目的探討姜黃素對(duì)宮頸癌干細(xì)胞增殖、聚集及化療敏感性的影響。方法用流式細(xì)胞術(shù)從人宮頸癌細(xì)胞株CASKI中分選CD133+的宮頸癌干細(xì)胞。將宮頸癌干細(xì)胞隨機(jī)分為七組,CC10組、CC20組、CC40組、CC60組、CC80組及CC100組分別給予終濃度為10、20、40、60、80、100 mg/m L姜黃素進(jìn)行培養(yǎng),對(duì)照組給予等量溶劑與培養(yǎng)基培養(yǎng)。用MTT法檢測(cè)各組細(xì)胞增殖抑制率。取對(duì)照組、CC60組細(xì)胞用無(wú)血清培養(yǎng)基培養(yǎng)1周,光鏡下計(jì)數(shù)聚集的直徑大于100μm的干細(xì)胞球個(gè)數(shù),流式細(xì)胞術(shù)檢測(cè)宮頸癌干細(xì)胞比例。取宮頸癌干細(xì)胞隨機(jī)分為BV組、CC組、BV+CC組、對(duì)照組四組,分別加入10 mg/m L貝伐單抗、60 mg/m L姜黃素、10 mg/m L BV+60 mg/m L姜黃素、等量溶劑與培養(yǎng)基,培養(yǎng)48 h后采用流式細(xì)胞術(shù)測(cè)算細(xì)胞凋亡率。結(jié)果 CC10組、CC20組、CC40組、CC60組、CC80組、CC100組細(xì)胞增殖抑制率均高于對(duì)照組(P均0.05);且隨著姜黃素濃度的增加,宮頸癌干細(xì)胞增殖抑制率逐漸升高(P均0.05)。CC60組直徑大于100μm的干細(xì)胞球個(gè)數(shù)少于對(duì)照組(P0.05),干細(xì)胞比例低于對(duì)照組(P0.05)。BV+CC組細(xì)胞凋亡率高于對(duì)照組、BV組、CC組(P均0.05),CC組高于對(duì)照組、BV組(P均0.05),BV組高于對(duì)照組(P均0.05)。結(jié)論姜黃素可抑制宮頸癌干細(xì)胞增殖,降低其聚集能力,增加其對(duì)貝伐單抗的治療敏感性。
[Abstract]:Objective to investigate the effect of curcumin on the proliferation of cervical cancer stem cells. Methods Cervical cancer stem cells with CD133 were selected from human cervical cancer cell line CASKI by flow cytometry. Cervical cancer stem cells were randomly divided into seven groups (CC10 group). CC20 group, CC40 group, CC60 group, CC80 group and CC100 group were given the final concentration of 10: 20, 40, 6080, respectively. #number0# mg/m L curcumin was cultured, the control group was cultured with the same amount of solvent and culture medium. The inhibition rate of cell proliferation was detected by MTT method. The cells in CC60 group were cultured in serum-free medium for 1 week, and the number of stem cell balls with diameter more than 100 渭 m was counted under light microscope. The proportion of cervical cancer stem cells was detected by flow cytometry. Cervical cancer stem cells were randomly divided into BV group (BV group) and control group (n = 4) with 10 mg/m L bevacizumab. 60 mg/m L curcumin 10 mg/m L BV 60 mg/m L curcumin, equal amount of solvent and medium. The apoptosis rate was measured by flow cytometry after 48 h culture. Results CC10 group CC20 group CC40 group CC60 group and CC80 group. The inhibition rate of cell proliferation in CC100 group was higher than that in control group (P < 0.05). With the increase of curcumin concentration, the proliferation inhibition rate of cervical cancer stem cells increased gradually (P < 0.05). The number of stem cells with diameter more than 100 渭 m in CC60 group was less than that in control group (P 0.05). The percentage of stem cells in CC group was lower than that in control group (P 0.05). The apoptosis rate in CC group was higher than that in control group (P < 0.05). Conclusion curcumin can inhibit the proliferation of cervical cancer stem cells, decrease its aggregation ability and increase its sensitivity to bevacizumab.
【作者單位】： 西安630醫(yī)院;西安交通大學(xué)第二附屬醫(yī)院;
【基金】：陜西省自然科學(xué)基金資助項(xiàng)目(2016JM8067)
【分類號(hào)】：R737.33
【正文快照】： 宮頸癌是發(fā)病率僅次于乳腺癌的女性惡性腫瘤,其發(fā)病率逐年升高,且發(fā)病年齡呈現(xiàn)年輕化趨勢(shì)[1]。目前宮頸癌新藥的研發(fā)多側(cè)重于預(yù)防。雖然傳統(tǒng)放化療可部分提高宮頸癌患者的生存率、改善患者預(yù)后,但仍無(wú)法有效控制宮頸癌的復(fù)發(fā)、轉(zhuǎn)移及治療耐藥[2,3]。貝伐單抗為一種人源單克隆

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本文編號(hào)：1421983