本文關(guān)鍵詞：子宮內(nèi)膜異位癥與免疫不孕的相關(guān)性研究　出處：《青島大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 子宮內(nèi)膜異位 免疫性不孕 性激素 免疫抗體 免疫細(xì)胞因子

【摘要】：目的:通過測定子宮內(nèi)膜異位癥與不孕患者內(nèi)分泌激素、免疫細(xì)胞及分子的表達(dá)水平,探討子宮內(nèi)膜異位癥與免疫性不孕的相關(guān)性。方法:選取臨沂市中醫(yī)醫(yī)院2013年10月-2015年01月收治的114例有子宮內(nèi)膜異位癥和(或)不孕患者。按其疾病類型分為單純子宮內(nèi)膜異位癥組(A組,38例)、單純不孕癥組(B組,38例)、子宮內(nèi)膜異位癥合并不孕組(C組,38例),同時以38例年齡相仿的健康女性作為對照組(D組)。采用化學(xué)發(fā)光免疫法測定患者的血清中的T、E2、PRL、FSH以及LH和INS水平。利用酶聯(lián)免疫吸附(ELISA)法檢測用每項觀察指標(biāo)的特定試劑盒對患者的ASAb、EMAb、AOAb、HCGAb以及ACAb,細(xì)胞因子IL-4、IL-6、IL-8、TNF-α、IFN,補(bǔ)體C3 C4,采用流式細(xì)胞術(shù)檢測患者外周血CD3+CD4+CD8+T細(xì)胞亞群。對不同組別的患者進(jìn)行以上指標(biāo)的比較,分析子宮內(nèi)膜異位癥與免疫性不孕的相關(guān)性。結(jié)果:⑴C組患者中的T、E2、PRL以及INS水平均顯著高于A組和B組患者,差異有統(tǒng)計學(xué)意義(P0.05),FSH和LH的水平3組無統(tǒng)計學(xué)差異(P0.05);對照組以及B組的PRL水平顯著低于A組患者,差異有統(tǒng)計學(xué)意義(P0.05),其他各指標(biāo)差異均無統(tǒng)計學(xué)意義(P0.05)。⑵C組的抗體總陽性率顯著高于其他3組,差異有統(tǒng)計學(xué)意義(P0.05),A組與B組之間的抗體陽性率差異無統(tǒng)計學(xué)意義(P0.05)。但均高于對照組(P0.05)。⑶C組的細(xì)胞因子的濃度滴度要顯著高于其他三組,差異具有統(tǒng)計學(xué)差異(P0.05),A組與B組之間的細(xì)胞因子滴度差異無統(tǒng)計學(xué)意義(P0.05),但均高于健康對照組,存在顯著差異(P0.05)。⑷C組中各個體液的免疫指標(biāo)中Ig G、Ig A、Ig M、C3、C4均顯著高于其他三組,且存在統(tǒng)計學(xué)差異,(P0.05)。同B組和健康對照組相比較,A組中各個體液免疫指標(biāo)中Ig G、Ig A、Ig M、C3、C4明顯偏高,且存在統(tǒng)計學(xué)上的差異,(P0.05)。B組和健康對照組不存在統(tǒng)計學(xué)上差異,(P0.05)。⑸C組中外周血中CD3+、CD4+、CD8+和CD4+/CD8+均顯著低于其他三組,存在顯著的統(tǒng)計學(xué)差異,(P0.05)。A組中外周血中CD3+、CD4+、CD8+和CD4+/CD8+均顯著低于B組和健康對照組,存在顯著的統(tǒng)計學(xué)差異(P0.05)。B組和健康對照組不存在統(tǒng)計學(xué)上差異,(P0.05)結(jié)論:⑴子宮內(nèi)膜異位合并不孕的患者性激素水平異常增高,說明激素的紊亂與子宮內(nèi)膜異位患者不孕密切相關(guān)。⑵子宮內(nèi)膜異位合并不孕的患者自身抗體增高,說明自身免疫損傷可能參與不孕的發(fā)生。⑶CD4+CD8+T細(xì)胞紊亂及IL-4 IL-6 IL-8炎性細(xì)胞因子導(dǎo)致的炎癥反應(yīng)及C3 C4的炎性介質(zhì)作用可促進(jìn)子宮內(nèi)膜異位癥患者不孕癥的發(fā)生⑷不孕癥的發(fā)生是多因素共同作用的結(jié)果,子宮內(nèi)膜異位致內(nèi)分泌激素及免疫細(xì)胞、免疫分子功能紊亂,造成內(nèi)分泌-免疫網(wǎng)絡(luò)失衡是導(dǎo)致不孕的關(guān)鍵因素
[Abstract]:Objective: to determine the expression of endocrine hormones, immunocytes and molecules in patients with endometriosis and infertility. To explore the relationship between endometriosis and immunological infertility methods: 114 cases of endometriosis and / or endometriosis were selected from October 2013 to January 2015 in Linyi traditional Chinese Medicine Hospital. Infertility patients. According to their disease type, divided into simple endometriosis group, group A. 38 cases of endometriosis with infertility, 38 cases of group B with simple infertility, 38 cases of group C with endometriosis and infertility). At the same time, 38 healthy women of the same age were used as the control group D to determine the serum TV-E2P PRL by chemiluminescent immunoassay. The levels of FSH and LH and INS were detected by Elisa. The Asab EMAbAbAOAb of patients was detected with a specific kit of each observed indicator. HCGAb and ACAB, cytokine IL-4, IL-6, IL-8, TNF- 偽, complement C3 C4. The subsets of CD3 CD4 CD8 T cells in peripheral blood were detected by flow cytometry. The above indexes were compared among different groups of patients. Results the levels of INS and INS in group 1: 1 were significantly higher than those in group A and group B. There was no significant difference in the levels of FSH and LH among the three groups. The level of PRL in control group and group B was significantly lower than that in group A (P 0.05). The total positive rate of antibody was significantly higher in P0.05C group than that in the other three groups (P 0.05). There was no significant difference in antibody positive rate between group A and group B, but the titer of cytokines in group A was significantly higher than that in group C (P 0.05). There was no significant difference in cytokine titer between group A and group B, but it was higher than that in healthy control group. There was significant difference in the immunological index of each humoral fluid in group P0.05. 4C, which was significantly higher than that in the other three groups, and there was statistical difference. Compared with group B and healthy control group, the levels of Ig G G, Ig MN C 3 C 3 C 4 in group A were significantly higher than those in group A, and there were statistical differences between them. There was no statistical difference between group B and control group in CD3 CD4 in peripheral blood. CD8 and CD4 / CD8 were significantly lower than the other three groups, there was a significant statistical difference in CD3 CD4 in peripheral blood of P0.05. A group. CD8 and CD4 / CD8 were significantly lower than group B and healthy control group, there was significant statistical difference. There was no statistical difference between group B and group B and healthy control group. Conclusion: the level of sex hormone in patients with endometriosis complicated with infertility is abnormally high. It is suggested that the disorder of hormones is closely related to infertility in endometriosis. 2. The autoantibodies of patients with endometriosis combined with infertility are higher than those of patients with endometriosis. It is suggested that autoimmune injury may be involved in the occurrence of infertility. 3CD4 CD8 T cell disorder and IL-4 IL-6 IL-8 inflammatory cytokines and C3. The inflammatory mediators of C4 can promote the occurrence of infertility in patients with endometriosis. 4 the occurrence of infertility is the result of multiple factors. Endocrine hormones and immune cells caused by endometriosis are the key factors leading to infertility due to the disturbance of immune molecule function and the imbalance of endocrine and immune network.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R711.71;R711.6
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本文編號：1416134