目的 有關(guān)細(xì)胞因子的基因多態(tài)性與川崎病易感性之間的關(guān)系仍未明了,因此本研究的目的是探討白細(xì)胞介素-1p基因啟動子位點的多態(tài)性的關(guān)聯(lián)-31T/C和第5外顯子基因+3953C/T與川崎病遺(KD)傳易感性之間的關(guān)系,及與川崎病并發(fā)冠脈損傷風(fēng)險間的關(guān)系。 方法 應(yīng)用聚合酶鏈反應(yīng)-限制性內(nèi)切酶片斷長度多態(tài)性分析(PCR-RFLP)方法結(jié)合瓊脂糖凝膠電泳技術(shù),對63例川崎病兒童和100名健康對照兒童進(jìn)行IL-1p基因+3953C/T位點及-31T/C位點的多態(tài)性分析。 結(jié)果 (1)KD組IL-1β基因啟動子-31T/C位點多態(tài)性的CC、CT、TT基因型分布頻率和C、T等位基因頻率與正常對照組比較差異無顯著性(χ2=5.294、1.332,P均>0.05)。KD組IL-1p基因第5外顯子+3953C/T位點多態(tài)性的CC、CT、TT基因型分布頻率和C、T等位基因頻率與正常對照組比較差異無顯著性(χ2=1.925、1.908,P均>0.05)。(2)KD組IL-1β基因啟動子-31T/C位點多態(tài)性的CC、CT、TT基因型分布頻率和C、T等位基因頻率在并發(fā)冠脈損傷和無冠脈損傷之間差異均無顯著...

【文章頁數(shù)】：49 頁

【學(xué)位級別】：碩士

【文章目錄】：
摘要 Abstract 目錄 Abbreviations Introduction 1 Subjects and methods
1.1 Materials
    1.1.1 Main reagents:see Table 1-1
    1.1.2 Instruments and manufactures:see Table 1-2
    1.1.3 Study population
1.2 Experimental methods
    1.2.1 Collection and processing of blood samples
    1.2.2 DNA extraction
    1.2.3 Principle and procedure
    1.2.4 SNP Genotyping
    1.2.5 SNP Genotyping for IL-1β polymorphisms
1.3 Statistical analysis 2 Results
2.1 Subjects completion
    2.1.1 Clinical characteristics of KD subjects:Table 2-1
2.2 PCR amplification results of IL-1β-31T/C and +3953C/T
2.3 IL-1β-31 T→C promoter polymorphism
2.4 IL-1β+3953 C→T promoter polymorphism
2.5 Hardy -Weinberg equilibrium testing
2.6 Genotypic and allelic frequencies of IL-1β-31 T→C promoter polymorphism
2.7 Genotypic and allelic frequencies of IL-1β+3953 C→T promoter
2.8 Association between Kawasaki disease patients with and without coronaryartery lesions (CAL)
    2.8.1 Genotypic and Allelic frequencies of the IL-1β-31 T→C polymorphism andCAL
    2.8.2 Genotypic and Allelic frequencies of the IL-1β+3953 C→T polymorphismand CAL 3 Discussion
3.1 Studies compatible with our results
3.2 Studies not in agreement with our results
3.3 Genome-Wide Linkage Study of KD
3.4 Study Limitation 4 Conclusion References Review
References Published work Acknowledgment



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