發(fā)布時間：2021-08-17 17:38

　　目的:研究維生素D（VD）及其受體（VDR）通過調(diào)節(jié)VEGF對新生大鼠支氣管肺發(fā)育不良（BPD）的調(diào)節(jié)作用和機制。方法:構(gòu)建BDP模型新生大鼠,分為對照組和BDP組。培養(yǎng)大鼠肺上皮Ⅱ型細胞（RLE-6TN）,RLE-6TN細胞分為VDR過表達（Le-VDR慢病毒感染）、VDR過表達對照組（Le-Ctrl）、VDR敲低（Sh-VDR感染）、VDR敲低對照組（Sh-Ctrl）,VD單獨處理,VEGF-A沉默組（si VEGF-A）; Le-VDR與si VEGF-A共處理（Le-VDR+si VEGF-A）;通過CCK-8檢測、流式細胞術(shù)、傷口愈合檢測和體外粘附檢測明確VD和VDR對肺泡上皮細胞的調(diào)節(jié)作用。RTq PCR檢測VDR和VEGF-A mRNA水平,Western blot檢測VDR和VEGF-A的蛋白水平。結(jié)果:與對照相比,BPD新生大鼠的VD水平和VDR表達降低（P<0.01）;在RLE-6TN細胞中過表達VDR,細胞增殖、遷移、粘附能力增加（均P<0.01）,凋亡率減少（P <0.01）;細胞中敲低VDR效果相反,VD刺激后細胞凋亡被抑制,細胞增殖、遷移和... 

【文章來源】：河北醫(yī)學(xué). 2020,26(10)

【文章頁數(shù)】：6 頁

【部分圖文】：

Reduced levels of VD and VDR in lung tissue of BPD

VDR affects the biological function of cells.(A) Cellswere infected by Le-Ctrl,Le-VDR,Sh-Ctrl,Sh-VDR,VDRprotein expression in cells were tested by Western blot;(B) Cellproliferation was detected by CCK-8 method;(C) Apoptosis was measured by flow cytometry;(D) Wound healing experiments to measure cell migration levels;(E) Cell adhesion experiment was used to detect the changes of adhesion levels.

VD and VDR regulated VEGF-A expression.(A) ThemRNA level of VEGF-A was detected by RT-q PCR;(B) The protein level of VEGF-A was detected by Western blot.*P<0.0 5,compared with the control group;#P<0.0 5,compared with Le-Ctrl;&P<0.0.5 compared with Sh-Ctrl.

【參考文獻】：
期刊論文
[1]支氣管肺發(fā)育不良早產(chǎn)兒嬰兒期預(yù)后研究[J]. 魏紅玲,邢燕,武慧,韓彤妍,童笑梅,周薇,樸梅花.  中國當(dāng)代兒科雜志. 2019(07)
[2]維生素D3對肺炎支原體感染小鼠肺組織TREM-1及HIF-1α表達的影響[J]. 曹波,齊小雪,張俊梅.  解剖科學(xué)進展. 2018(06)



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