【摘要】：目的:1.檢測(cè)不同時(shí)間點(diǎn)的RDS大白鼠和健康大白鼠的血清甲狀腺素的變化規(guī)律。2.檢測(cè)不同的時(shí)間點(diǎn)RDS大白鼠及健康大白鼠肺組織中KLF2蛋白和KLF2 mRNA的表達(dá),并觀察大鼠肺組織病理形態(tài)學(xué)改變。進(jìn)而研究klf2轉(zhuǎn)錄因子在RDS大鼠肺組織的表達(dá)變化及甲狀腺素水平的變化,探討甲狀腺素與klf2之間的相互作用,為臨床用肺泡表面活性物質(zhì)治療不敏感的NRDS提供可能的理論依據(jù)。方法:SD雄性大鼠50只,隨機(jī)分為4組,油酸組39只,再分為3小時(shí)、18小時(shí)和24小時(shí)組各13只,對(duì)照組11只不做任何處理。油酸組39只SD大鼠尾靜脈注射0.17mg/Kg油酸建立RDS大鼠模型,分別于注射后3h、18h、24h用電化學(xué)發(fā)光法檢測(cè)血清甲狀腺素水平,同樣用Western blot和RT-PCR方法檢測(cè)RDS大白鼠以上時(shí)間段及非RDS大白鼠肺組織中KLF2蛋白和KLF2 mRNA的表達(dá),并用組化免疫觀察大鼠肺組織病理形態(tài)學(xué)改變。結(jié)果:1.油酸3h組、18h組中的血清FT4與正常對(duì)照組比較P0.01;油酸3h組中T4與正常對(duì)照組比較P0.01,有顯著統(tǒng)計(jì)學(xué)意義;油酸18h組中T4數(shù)據(jù)與正常對(duì)照組比較P0.05,有統(tǒng)計(jì)學(xué)意義;2油酸3h組肺組織中的KLF2蛋白量與健康雄性SD大鼠肺組織中KLF2蛋白量數(shù)據(jù)對(duì)照比較,P0.05,有統(tǒng)計(jì)學(xué)意義;而18h組、24h無(wú)統(tǒng)計(jì)學(xué)意義,表明,在本組動(dòng)物實(shí)驗(yàn)中,油酸所致RDS大鼠肺組織在3小時(shí)相比正常組KLF2蛋白量是減少的。3油酸24h組肺組織中的KLF2mRNA與健康雄性SD大鼠肺組織中KLF2m RNA數(shù)據(jù)對(duì)照比較,P0.05;有統(tǒng)計(jì)學(xué)意義;而3h組、18h組無(wú)統(tǒng)計(jì)學(xué)意義,表明,在本組動(dòng)物實(shí)驗(yàn)中,油酸所致RDS大鼠肺組織在24小時(shí)相比正常組KLF2mRNA量有增多的趨勢(shì)。結(jié)論:1.FT4、T4甲狀腺激素在RDS大鼠3h、18h有明顯下降趨勢(shì),呼吸窘迫綜合癥與血清甲狀腺素水平有相關(guān)性。2.油酸所致RDS大鼠肺組織在3小時(shí)相比正常組KLF2蛋白量是減少的,呼吸窘迫綜合癥與KLF2蛋白有一定相關(guān)性。3.在RDS大鼠中血清FT4、T4是降低的,油酸3小時(shí)組肺組織KLF2蛋白量是減少的,因此FT4、T4與KLF2有一定的相關(guān)性。
[Abstract]:Objective: 1. To detect the changes of serum thyroxine in RDS rats and healthy rats at different time points. 2. The expression of KLF2 protein and KLF2 mRNA in lung tissues of RDS rats and healthy rats were detected at different time points, and the pathological changes of lung tissues were observed. Furthermore, the expression of klf2 transcription factors and the changes of thyroxine level in lung tissue of RDS rats were studied, and the interaction between thyroxine and klf2 was discussed, which provided a possible theoretical basis for the clinical treatment of insensitive NRDS with pulmonary surfactant. Methods: fifty male SD rats were randomly divided into 4 groups: oleic acid group (n = 39) and oleic acid group (n = 39). In the oleic acid group, 39 SD rats were injected with 0.17mg/Kg oleic acid into the tail vein to establish the model of RDS. The serum thyroxine levels were detected by electrochemiluminescence (ECL) at 3 h, 18 h and 24 h after injection, respectively. Western blot and RT-PCR methods were used to detect the expression of KLF2 protein and KLF2 mRNA in the lung tissues of RDS rats and non-RDS rats. The histopathological changes of lung tissues were observed by immunohistochemistry. Results: 1. The serum FT4 in 3 h group and 18 h group was significantly higher than that in normal control group (P 0.01), and that in 3 h group of oleic acid group was significantly higher than that in normal control group (P 0.01), and that in 3 h group of oleic acid group was significantly higher than that in control group. Compared with the normal control group, the content of KLF2 protein in the lung tissue of 3 h oleic acid group was significantly higher than that in the normal control group (P0.05), and the content of KLF2 protein in the lung tissue of the male SD rats was significantly higher than that in the control group (P0.05). In 18h group, there was no statistical significance at 24 h, which indicated that in this group of animal experiments, The content of KLF2 protein in lung tissue of RDS rats induced by oleic acid was lower than that of normal group at 3 hours. 3 KLF2mRNA in lung tissue of 24 h oleic acid group was compared with KLF2m RNA data in lung tissue of healthy male SD rats (P 0.05). However, there was no significant difference between the 3 h group and the 18 h group, which indicated that the lung tissue of oleic acid-induced RDS rats had a tendency to increase compared with the normal group at 24 hours. Conclusions: 1. FT4 T4 thyroid hormone decreased significantly in RDS rats at 3 h and 18 h, and respiratory distress syndrome was correlated with serum thyroxine level. 2. The content of KLF2 protein in lung tissue of RDS rats induced by oleic acid was decreased in 3 hours compared with that in normal group. Respiratory distress syndrome was associated with KLF2 protein. Serum FT4,T4 was decreased in RDS rats and KLF2 protein in lung tissue was decreased in 3 h oleic acid group. Therefore, there was a certain correlation between FT4,T4 and KLF2.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R722.1

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