【摘要】：目的通過(guò)檢測(cè)Toll樣受體9(Toll like receptor 9,TLR9)、髓樣分化因子88(myeloid different factory88,My D88)及核轉(zhuǎn)錄因子Kappa-B(nuclear factor kappa-B,NF-κB)m RNA在過(guò)敏性紫癜(Henoch-Schonlein purpura,HSP)患兒外周血單核細(xì)胞的表達(dá),同時(shí)檢測(cè)HSP患兒血漿細(xì)胞因子白介素6(interleukin-6,IL-6)、腫瘤壞死因子α(Tumor Necrosis Factor alpha,TNF-α)水平,探討TLR9-My D88-NF-κB信號(hào)通路在HSP發(fā)病機(jī)制的作用,為治療HSP尋找免疫上游靶點(diǎn)提供基礎(chǔ)。方法選取2015年6月至2016年12月鄭州大學(xué)第二附屬醫(yī)院兒科門診及住院急性期HSP患兒40例作為研究對(duì)象,另選取同期在兒科門診定期復(fù)查已痊愈HSP兒童35例作為正常對(duì)照組。采用ELISA法檢測(cè)血漿IL-6、TNF-α水平,兩組間比較采用獨(dú)立樣本t檢驗(yàn),相關(guān)性分析采用Pearson相關(guān)檢驗(yàn)。結(jié)果1.HSP患兒外周血單核細(xì)胞TLR9、My D88及NF-κB m RNA表達(dá)均顯著高于對(duì)照組(t=5.68,P0.01;t=5.42,P0.01;t=112.95,P0.01),差異有統(tǒng)計(jì)學(xué)意義;2.HSP組血漿IL-6、TNF-α水平較對(duì)照組水平顯著增高(t=5.11,P0.01;t=9.26,P0.01),差別有統(tǒng)計(jì)學(xué)意義;3.HSP組中TLR9與My D88,TLR9與NF-κB,My D88與NF-κB,NF-κB與IL-6以及NF-κB與TNF-α均存在線性正相關(guān)關(guān)系(r=1,P0.01;r=0.98,P0.01;r=0.98,P0.01;r=0.98,P0.01;r=0.97,P0.01)。結(jié)論HSP患兒TLR9、My D88、NF-κB m RNA、IL-6、TNF-α水平均較對(duì)照組兒童顯著升高。免疫上游因子TLR9、My D88、NF-κB對(duì)免疫下游細(xì)胞因子IL-6、TNF-α釋放具有同步關(guān)聯(lián)性。TLR9-My D88-NF-κB信號(hào)通路參與HSP發(fā)病,并在其中起重要作用。
[Abstract]:Objective to detect the expression of Toll like receptor 9 (Toll like receptor 9 TLR9, myeloid differentiation factor 88 (myeloid different factory88,My D88 and nuclear transcription factor Kappa-B (nuclear factor kappa-B,NF- 魏 B) m RNA in allergic purpura (Henoch-Schonlein purpura,). The levels of plasma cytokine interleukin-6 (interleukin-6,IL-6), tumor necrosis factor 偽 (Tumor Necrosis Factor alpha,TNF- 偽 (TNF- 偽) and monocytes in peripheral blood of children with HSP were detected. To investigate the role of TLR9-My D88-NF- 魏 B signaling pathway in the pathogenesis of HSP, and to provide a basis for the search of upstream targets for the treatment of HSP. Methods from June 2015 to December 2016, 40 children with HSP in pediatrics outpatient and hospitalized acute phase in the second affiliated Hospital of Zhengzhou University were selected as the study objects, and 35 children who had recovered HSP in the same period were selected as the normal control group. The plasma IL-6,TNF- 偽 levels were detected by ELISA method, independent t-test and Pearson correlation test were used for the comparison between the two groups. Results the expression of TLR9,My D88 and NF- 魏 B m RNA in peripheral blood monocytes of children with 1.HSP was significantly higher than that of the control group (t = 5.68, P 0.01, P = 5.42, P 0.01, n = 112.95, P 0.01), and there was a significant difference in the expression of TLR9,My D88 and NF- 魏 B m RNA between the two groups. The level of plasma IL-6,TNF- 偽 in 2.HSP group was significantly higher than that in control group (t = 5.11, P 0.01, P = 9.26, P 0.01), and the difference was statistically significant. There was a positive linear correlation between TLR9 and My D88 TLR9 and NF- 魏 By D88 with NF- 魏 B and IL-6 and NF- 魏 B with TNF- 偽 in 3.HSP group. (P 0.01, P 0. 97, P 0. 01). Conclusion the level of NF- 魏 B m RNA,IL-6,TNF- 偽 in TLR9,My D 88 in HSP children is significantly higher than that in control children. The upstream factor TLR9,My D88 NF- 魏 B has a synchronous correlation with the release of IL-6,TNF- 偽. The TLR9-My D88-NF- 魏 B signaling pathway is involved in the pathogenesis of HSP and plays an important role in it.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R725.5

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