輔助性T細胞相關細胞因子在兒童急性免疫性血小板減少性紫癜中的變化及意義
發(fā)布時間：2018-10-26 16:19
【摘要】：目的 通過檢測急性免疫性血小板減少性紫癜(immune thrombocytopenic purpura ITP)患兒輔助性T(Th)淋巴細胞相關細胞因子γ-干擾素(IFN-γ)、白介素4(IL-4)、B細胞特異單克隆鼠白血病病毒整合位點基因(Bmi-1)及白介素17(IL-17)mRNA表達的變化,分析探討急性ITP患兒Th細胞相關細胞因子功能變化及其相關性,以期進一步明確Th細胞相關細胞因子在急性ITP患兒發(fā)病中的作用。 方法 本實驗設為實驗組與對照組。實驗組36例急性ITP患兒均源于新鄉(xiāng)醫(yī)學院第一附屬醫(yī)院兒科,2012年1月-2012年9月期間住院和門診患兒,均符合急性ITP診斷標準；對照組為我院同期小兒外科擇期手術患兒26例,采集血標本前2周內均無感染史；兩組采集血標本前4周內均無輸血、糖皮質激素使用及疫苗接種史,均為河南地區(qū)漢族兒童,性別、年齡均無統(tǒng)計學差異(P0.05)；均獲得其家屬知情同意。 采用流式細胞儀(fluorescence-activated cell sorting,FACS)檢測實驗組與對照組兒童外周血T細胞亞群比例；采用RT-PCR方法檢測外周血淋巴細胞中TFN-γ、 Bmi-1、IL-4、IL-17Th相關的細胞因子mRNA的表達；并采用t檢驗和直線相關分析進行分析比較。 結果 1、實驗組CD3+細胞百分比為60.54±3.46,CD4+細胞百分比為30.07±0.79,CD8+細胞百分比為25.23±2.57,CD4+/CD8+細胞比值為1.12±0.19,對照組CD3+細胞百分比為63.73±4.03,CD4+細胞百分比為35.41±4.76,CD8+細胞百分比為21.62±2.53,CD4+/CD8+細胞比值為1.64±0.28；實驗組與對照組比較,CD3+細胞低于對照組(t=3.36,P0.001),CD4+細胞明顯低于對照組(t=8.30,P0.001),CD8+細胞明顯高于對照組(t=5.49,P0.001),CD4+/CD8+明顯低于對照組(t=8.72,P0.001)。 2、實驗組外周血淋巴細胞中IFN-ymRNA為3.84±0.43, IL-4mRNA為1.44±0.39, Bmi-1mRNA為2.63±0.54,IL-17mRNA為4.69±0.58;對照組外周血淋巴細胞中IFN-ymRNA為2.88±0.57,IL-4mRNA為1.87±0.34,Bmi-1mRNA為3.91±0.92,IL-17mRNA為3.79±0.82；；實驗組與對照組比較,IFN-γ明顯高于對照組(t=7.56,P0.001),IL-4明顯低于對照組(t=4.52,P0.001)；Bmi-1明顯低于對照組(t=6.88,P0.001),IL-17明顯高于對照組(t=5.07,P0.01)。 3、實驗組外周血淋巴細胞中IFN-γ mRNA表達與[L-4mRNA表達呈負相關(r=-0.667,P0.001),實驗組外周血淋巴細胞中IL-4mRNA表達與Bmi-1基因mRNA表達呈正相關(r=0.776,P0.001)；實驗組外周血淋巴細胞中IFN-γ mRNA表達與IL-17mRNA表達呈正相關(r=0.712,P0.001)；實驗組外周血淋巴細胞中IFN-γ mRNA表達與Bmi-1mRNA表達無相關性(r=-0.206, P0.05)；實驗組外周血淋巴細胞中IL-17mRNA表達與Bmi-1mRNA表達無相關性(r=-0.214,P0.05)；實驗組外周血淋巴細胞中IL-17mRNA表達與IL-4mRNA表達無相關性(r=-0.220,P0.05)。 結論 1急性ITP患兒存在細胞免疫紊亂,Th/Ts細胞失衡,且Th細胞功能紊亂,Th1/Th2比例失調,可能是Th1發(fā)揮優(yōu)勢； 2Bmi-1水平降低可能參與急性ITP的發(fā)病; 3Th17可能通過促進IL-17的分泌在急性ITP的發(fā)病中起作用。
[Abstract]:Objective to detect the cytokines interferon 緯 (IFN- 緯) and interleukin-4 (IL-4) of helper T (Th) lymphocytes in children with acute immune thrombocytopenic purpura (immune thrombocytopenic purpura ITP). The changes of expression of Bmi-1 and IL-17 mRNA in B cell-specific monoclonal mouse leukemia virus (MMLV) were analyzed to investigate the changes of cytokine function of Th cells in children with acute ITP. To further clarify the role of Th cell-related cytokines in the pathogenesis of acute ITP children. Methods the experiment was divided into experimental group and control group. The 36 children with acute ITP in the experimental group were all from paediatrics of the first affiliated Hospital of Xinxiang Medical College. From January 2012 to September 2012, all the hospitalized and outpatient children met the diagnostic criteria of acute ITP. The control group consisted of 26 children undergoing elective operation in pediatric surgery in the same period. There was no history of infection within 2 weeks before blood samples were collected. No blood transfusion, glucocorticoid use and vaccination history were observed in the two groups in the first 4 weeks. There was no significant difference in sex and age between the two groups (P0.05); the informed consent of their families was obtained. The percentage of T lymphocyte subsets in peripheral blood of children in experimental group and control group was detected by flow cytometry (fluorescence-activated cell sorting,FACS). The expression of TFN- 緯 and Bmi-1,IL-4,IL-17Th related cytokines mRNA in peripheral blood lymphocytes was detected by RT-PCR method and compared with t test and linear correlation analysis. Results 1 the percentage of CD3 cells in the experimental group was 60.54 鹵3.46, the percentage of CD4 cells was 30.07 鹵0.79 and the percentage of CD8 cells was 25.23 鹵2.57%. The ratio of CD4 / CD8 cells was 1.12 鹵0.19. In the control group, the percentage of CD3 cells was 63.73 鹵4.03, the percentage of CD4 cells was 35.41 鹵4.76, the percentage of CD8 cells was 21.62 鹵2.53, the ratio of CD4 / CD8 cells was 1.64 鹵0.28; Compared with the control group, the CD3 cells, CD4 cells and CD8 cells in the experimental group were significantly lower than those in the control group (t = 3.36, P 0.001), and in the control group, the CD8 cells were significantly higher than those in the control group (t = 5.49, P 0.001). The ratio of CD4 / CD8 was significantly lower than that of the control group (t = 8.72, P 0.001). 2. The IFN-ymRNA, IL-4mRNA and Bmi-1mRNA in peripheral blood lymphocytes of the experimental group were 3.84 鹵0.43, 1.44 鹵0.39 and 2.63 鹵0.54 respectively, which were 4.69 鹵0.58; In the control group, the level of IFN-ymRNA in peripheral blood lymphocytes was 2.88 鹵0.57, IL-4 mRNA was 1.87 鹵0.34, Bmi-1 mRNA was 3.91 鹵0.92 and IL-17 mRNA was 3.79 鹵0.82in peripheral blood lymphocytes. Compared with the control group, the IFN- 緯 of the experimental group was significantly higher than that of the control group (t = 7.56, P 0.001), and the IL-4 was significantly lower than that of the control group (t = 4.52, P 0.001). Bmi-1 was significantly lower than that in the control group (t = 6.88, P 0.001), and IL-17 was significantly higher than that in the control group (t = 5.07, P 0.01). 3. The expression of IFN- 緯 mRNA was negatively correlated with the expression of [L-4mRNA] in peripheral blood lymphocytes of experimental group (r-0.667p0.001), and the expression of IL-4mRNA in peripheral blood lymphocytes of experimental group was positively correlated with the expression of Bmi-1 gene mRNA (r-0.776). (P0.001); There was a positive correlation between the expression of IFN- 緯 mRNA and IL-17mRNA in peripheral blood lymphocytes of the experimental group (r = 0.712, P 0.001), but no correlation between the expression of IFN- 緯 mRNA and the expression of Bmi-1mRNA in the peripheral blood lymphocytes of the experimental group (r = 0.206, P 0.05). There was no correlation between IL-17mRNA expression and Bmi-1mRNA expression in peripheral blood lymphocytes of experimental group (r-0.2145P0.05), but there was no correlation between IL-17mRNA expression and IL-4mRNA expression in peripheral blood lymphocytes of experimental group (r-0.220p0.05). Conclusion (1) Children with acute ITP have cellular immune disorder, Th/Ts cell imbalance, Th cell dysfunction and Th1/Th2 imbalance, which may be the advantage of Th1. The decrease of 2Bmi-1 level may be involved in the pathogenesis of acute ITP and 3Th17 may play a role in the pathogenesis of acute ITP by promoting the secretion of IL-17.
【學位授予單位】：新鄉(xiāng)醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R725.5
[Abstract]:Objective to detect the cytokines interferon 緯 (IFN- 緯) and interleukin-4 (IL-4) of helper T (Th) lymphocytes in children with acute immune thrombocytopenic purpura (immune thrombocytopenic purpura ITP). The changes of expression of Bmi-1 and IL-17 mRNA in B cell-specific monoclonal mouse leukemia virus (MMLV) were analyzed to investigate the changes of cytokine function of Th cells in children with acute ITP. To further clarify the role of Th cell-related cytokines in the pathogenesis of acute ITP children. Methods the experiment was divided into experimental group and control group. The 36 children with acute ITP in the experimental group were all from paediatrics of the first affiliated Hospital of Xinxiang Medical College. From January 2012 to September 2012, all the hospitalized and outpatient children met the diagnostic criteria of acute ITP. The control group consisted of 26 children undergoing elective operation in pediatric surgery in the same period. There was no history of infection within 2 weeks before blood samples were collected. No blood transfusion, glucocorticoid use and vaccination history were observed in the two groups in the first 4 weeks. There was no significant difference in sex and age between the two groups (P0.05); the informed consent of their families was obtained. The percentage of T lymphocyte subsets in peripheral blood of children in experimental group and control group was detected by flow cytometry (fluorescence-activated cell sorting,FACS). The expression of TFN- 緯 and Bmi-1,IL-4,IL-17Th related cytokines mRNA in peripheral blood lymphocytes was detected by RT-PCR method and compared with t test and linear correlation analysis. Results 1 the percentage of CD3 cells in the experimental group was 60.54 鹵3.46, the percentage of CD4 cells was 30.07 鹵0.79 and the percentage of CD8 cells was 25.23 鹵2.57%. The ratio of CD4 / CD8 cells was 1.12 鹵0.19. In the control group, the percentage of CD3 cells was 63.73 鹵4.03, the percentage of CD4 cells was 35.41 鹵4.76, the percentage of CD8 cells was 21.62 鹵2.53, the ratio of CD4 / CD8 cells was 1.64 鹵0.28; Compared with the control group, the CD3 cells, CD4 cells and CD8 cells in the experimental group were significantly lower than those in the control group (t = 3.36, P 0.001), and in the control group, the CD8 cells were significantly higher than those in the control group (t = 5.49, P 0.001). The ratio of CD4 / CD8 was significantly lower than that of the control group (t = 8.72, P 0.001). 2. The IFN-ymRNA, IL-4mRNA and Bmi-1mRNA in peripheral blood lymphocytes of the experimental group were 3.84 鹵0.43, 1.44 鹵0.39 and 2.63 鹵0.54 respectively, which were 4.69 鹵0.58; In the control group, the level of IFN-ymRNA in peripheral blood lymphocytes was 2.88 鹵0.57, IL-4 mRNA was 1.87 鹵0.34, Bmi-1 mRNA was 3.91 鹵0.92 and IL-17 mRNA was 3.79 鹵0.82in peripheral blood lymphocytes. Compared with the control group, the IFN- 緯 of the experimental group was significantly higher than that of the control group (t = 7.56, P 0.001), and the IL-4 was significantly lower than that of the control group (t = 4.52, P 0.001). Bmi-1 was significantly lower than that in the control group (t = 6.88, P 0.001), and IL-17 was significantly higher than that in the control group (t = 5.07, P 0.01). 3. The expression of IFN- 緯 mRNA was negatively correlated with the expression of [L-4mRNA] in peripheral blood lymphocytes of experimental group (r-0.667p0.001), and the expression of IL-4mRNA in peripheral blood lymphocytes of experimental group was positively correlated with the expression of Bmi-1 gene mRNA (r-0.776). (P0.001); There was a positive correlation between the expression of IFN- 緯 mRNA and IL-17mRNA in peripheral blood lymphocytes of the experimental group (r = 0.712, P 0.001), but no correlation between the expression of IFN- 緯 mRNA and the expression of Bmi-1mRNA in the peripheral blood lymphocytes of the experimental group (r = 0.206, P 0.05). There was no correlation between IL-17mRNA expression and Bmi-1mRNA expression in peripheral blood lymphocytes of experimental group (r-0.2145P0.05), but there was no correlation between IL-17mRNA expression and IL-4mRNA expression in peripheral blood lymphocytes of experimental group (r-0.220p0.05). Conclusion (1) Children with acute ITP have cellular immune disorder, Th/Ts cell imbalance, Th cell dysfunction and Th1/Th2 imbalance, which may be the advantage of Th1. The decrease of 2Bmi-1 level may be involved in the pathogenesis of acute ITP and 3Th17 may play a role in the pathogenesis of acute ITP by promoting the secretion of IL-17.
【學位授予單位】：新鄉(xiāng)醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R725.5
【參考文獻】
相關期刊論文 前10條
1 馮艷;宋立兵;郭寶紅;廖雯婷;李滿枝;劉萬里;曾木圣;張玲;;Bmi-1在乳腺癌組織中的表達及意義[J];癌癥;2007年02期
2 何麗雅;黃紹良;趙小媛;賴冬波;;特發(fā)性血小板減少性紫癜患兒Th1/Th2細胞因子水平及其臨床意義[J];臨床兒科雜志;2008年03期
3 馮建軍;楊林花;陳劍芳;來曉煒;馬艷萍;馬梁明;侯麗虹;申秀敏;;ITP血小板特異性抗體和T淋巴細胞亞群及NK細胞變化的意義探討[J];臨床血液學雜志;2009年01期
4 林靜華;蔡應木;陳彥融;焦曉陽;方琳麗;;ITP患者外周血T淋巴細胞亞群的變化及臨床意義[J];中國熱帶醫(yī)學;2008年12期
5 邢同京,章廉,駱抗先;Th細胞分化調節(jié)機制研究進展[J];上海免疫學雜志;2000年02期
6 黎翠翠;蔣莉;陳曲波;;Th22及其效應因子生物學特性相關研究[J];現(xiàn)代免疫學;2011年03期
7 張景榮;靳蓉;王健;朱莉;向慧;梁筱靈;;急性特發(fā)性血小板減少性紫癜患兒細胞免疫功能的變化[J];實用兒科臨床雜志;2010年23期
8 瞿文;伍星;王s，
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