3、7型腺病毒纖毛與六鄰體基因序列分析
發(fā)布時(shí)間：2018-09-13 13:16
【摘要】：1背景：2009年6月至2011年2月課題組從呼吸道感染住院患兒中共檢出人腺病毒(Human adenovirus, HAdV)陽性標(biāo)本105份,其中3型55例,7型40例,5型4例,1型4例,55型1例,2型1例,而2010-2011年冬,腺病毒感染率激增。由于HAdV易發(fā)生血清型間重組現(xiàn)象。為進(jìn)一步明確是否因重組造成了HAdV的感染率激增,本研究分析比較前期臨床分離的3,7型HAdV纖毛(Fiber)與六鄰體(Hexon)蛋白的基因序列。 方法：從2009年6月～2011年5月,收集因呼吸道感染在重慶醫(yī)科大學(xué)附屬兒童醫(yī)院呼吸病房住院患兒鼻咽抽吸物(Nasopharyngeal aspirates, NAPs)1780例。所有標(biāo)本進(jìn)行HAdV PCR擴(kuò)增,腺病毒陽性者測(cè)序,基因庫進(jìn)行比對(duì)確定血清型。對(duì)所有HAdV-B陽性分別進(jìn)行Hexon高變區(qū)(Highly variable region, HVR)及纖毛蛋白基因PCR擴(kuò)增測(cè)序,并對(duì)3型和7型腺病毒進(jìn)行進(jìn)化樹分析(Phylogeny analysis)及相似度分析(Similarity analysis)。根據(jù)已確定的腺病毒血清型進(jìn)行相關(guān)臨床資料分析,統(tǒng)計(jì)分析軟件使用SPSS17.0。 結(jié)果：重慶地區(qū)引起兒童急性呼吸道感染(Acute respiratory tract infection, ARTI)的腺病毒血清型主要為3型和7型。Logestic回歸分析發(fā)現(xiàn),HAdV-7相對(duì)于HAdV-3更容易致重癥肺炎(OR=0.089,95%CI0.021-0.375)。建立所有B組HAdV進(jìn)化樹,并未發(fā)現(xiàn)重組現(xiàn)象。SimPlot相似度分析發(fā)現(xiàn)HAdV-3和HAdV-7序列差異集中在纖毛蛋白(Fiber)的基因序列。 結(jié)論:HAdV-3和HAdV-7是引起重慶地區(qū)兒童ARTI的主要血清型,但兩者引起肺炎嚴(yán)重度不同�；蛐蛄蟹治鎏崾纠w毛蛋白序列差異可能導(dǎo)致HAdV-3和HAdV-7腺病毒相關(guān)肺炎嚴(yán)重度差異。
[Abstract]:Background: from June 2009 to February 2011, 105 human adenovirus (Human adenovirus, HAdV) positive specimens were detected from hospitalized children with respiratory tract infection, including 55 cases of type 3, 40 cases of type 7, 4 cases of type 5, 1 case of type 55, 1 case of type 2, and 1 case of type 2 in winter 2010-2011. Adenovirus infection rates have soared. HAdV is prone to serotypic recombination. In order to find out whether the infection rate of HAdV increased rapidly due to recombination, this study compared the gene sequences of the ciliated (Fiber) of type 3 HAdV and the (Hexon) protein of hexahedron. Methods: from June 2009 to May 2011, 1780 cases of nasopharyngeal aspiration (Nasopharyngeal aspirates, NAPs) were collected in respiratory ward of Children's Hospital affiliated to Chongqing Medical University. All the specimens were amplified by HAdV PCR, the adenovirus positive individuals were sequenced, and the gene bank was compared to determine the serotype. All HAdV-B positives were amplified and sequenced by Hexon hypervariable region (Highly variable region, HVR) and ciliated protein gene PCR respectively. The 3 and 7 adenovirus were analyzed by phylogenetic tree analysis (Phylogeny analysis) and similarity analysis (Similarity analysis). According to the analysis of the clinical data of adenovirus serotypes, SPSS17.0. is used in the statistical analysis software. Results: the serotypes of adenovirus in children with acute respiratory tract infection (Acute respiratory tract infection, ARTI) were mainly serotype 3 and type 7. Logestic regression analysis showed that HAdV-7 was more likely to cause severe pneumonia (OR=0.089,95%CI0.021-0.375) than that of HAdV-3. All group B HAdV phylogenetic trees were established, and no recombination phenomenon was found. SimPlot similarity analysis showed that the difference between HAdV-3 and HAdV-7 sequence was concentrated in the gene sequence of ciliated protein (Fiber). ConclusionHAdV-3 and HAdV-7 are the main serotypes of ARTI in children in Chongqing area, but the severity of pneumonia is different between them. Gene sequence analysis suggested that the difference of ciliated protein sequence might lead to the difference of severity between HAdV-3 and HAdV-7 adenovirus associated pneumonia.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R725.1
本文編號(hào)：2241280
[Abstract]:Background: from June 2009 to February 2011, 105 human adenovirus (Human adenovirus, HAdV) positive specimens were detected from hospitalized children with respiratory tract infection, including 55 cases of type 3, 40 cases of type 7, 4 cases of type 5, 1 case of type 55, 1 case of type 2, and 1 case of type 2 in winter 2010-2011. Adenovirus infection rates have soared. HAdV is prone to serotypic recombination. In order to find out whether the infection rate of HAdV increased rapidly due to recombination, this study compared the gene sequences of the ciliated (Fiber) of type 3 HAdV and the (Hexon) protein of hexahedron. Methods: from June 2009 to May 2011, 1780 cases of nasopharyngeal aspiration (Nasopharyngeal aspirates, NAPs) were collected in respiratory ward of Children's Hospital affiliated to Chongqing Medical University. All the specimens were amplified by HAdV PCR, the adenovirus positive individuals were sequenced, and the gene bank was compared to determine the serotype. All HAdV-B positives were amplified and sequenced by Hexon hypervariable region (Highly variable region, HVR) and ciliated protein gene PCR respectively. The 3 and 7 adenovirus were analyzed by phylogenetic tree analysis (Phylogeny analysis) and similarity analysis (Similarity analysis). According to the analysis of the clinical data of adenovirus serotypes, SPSS17.0. is used in the statistical analysis software. Results: the serotypes of adenovirus in children with acute respiratory tract infection (Acute respiratory tract infection, ARTI) were mainly serotype 3 and type 7. Logestic regression analysis showed that HAdV-7 was more likely to cause severe pneumonia (OR=0.089,95%CI0.021-0.375) than that of HAdV-3. All group B HAdV phylogenetic trees were established, and no recombination phenomenon was found. SimPlot similarity analysis showed that the difference between HAdV-3 and HAdV-7 sequence was concentrated in the gene sequence of ciliated protein (Fiber). ConclusionHAdV-3 and HAdV-7 are the main serotypes of ARTI in children in Chongqing area, but the severity of pneumonia is different between them. Gene sequence analysis suggested that the difference of ciliated protein sequence might lead to the difference of severity between HAdV-3 and HAdV-7 adenovirus associated pneumonia.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R725.1
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 譚耀駒;謝菲;;腺病毒分型診斷研究進(jìn)展[J];廣東醫(yī)學(xué);2008年01期
，本文編號(hào)：2241280
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