【摘要】：目的：研究代表不同胎齡新生兒缺氧缺血性腦病(hypoxic ischemic encephalopathy, HIE)的模型鼠腦組織中谷氨酸(glutamate, Glu)含量變化的時(shí)間依從性及干預(yù)措施。方法：將128只新生SD大鼠按出生日齡(postnatal age, P)分為P2、P6、P12、P184組,每組32只；各組按不同干預(yù)措施分為缺氧缺血性腦損傷(hypoxic ischemic brain damage, HIBD)模型對(duì)照組(H)、α-氨基羥甲基惡唑丙酸(AMPA)干預(yù)組(A)、GYKI52466干預(yù)組(G)與空白對(duì)照組(N),每組8只。除N組外,各組參照Rice方法建立HIBD標(biāo)準(zhǔn)模型,A、G組于造摸后立即給藥。用藥6小時(shí)后采集標(biāo)本,檢測(cè)各組腦組織Glu含量,同時(shí)做病理學(xué)檢查。結(jié)果：各日齡組腦組織中Glu含量比較,Glu含量與日齡呈負(fù)相關(guān)(r=-0.81,p0.01),P2組含量最高,與P6、P12、P18組比較均有顯著性差異[(670.2±139.4)μmol/gprot.vs.(576±139) μmol/gprot.,(670.2±139.4)μmol/gprot.vs.(441.9±114.9) μmol/gprot.,(670.2±139.4)μmol/gprot.vs.(287.0±82.8) μmol/gprot.,Q=3.88,9.41,15.80,P0.05或P0.01]。各日齡組內(nèi)比較,G組腦組織中Glu含量明顯低于A組、H組和N組[(348.6±128.5)μmol/gprot. vs.(608.6±176.7)μmol/gprot.,(348.6±128.5)μmol/gprot.vs.(554.2±206.2)μmol/gprot.,(348.6±128.5)μmol/gprot.vs.(463.8±167.1) μmol/gprot.,Q=10.72,8.49,4.75,P均0.01]。病理學(xué)改變與G1u含量變化呈一致性。結(jié)論：代表不同胎齡新生兒腦發(fā)育水平的不同日齡新生大鼠腦組織中,Glu的含量具有時(shí)間依從性,與日齡呈負(fù)相關(guān)；HIBD可導(dǎo)致Glu含量明顯增加而加重腦損傷；而GYKI52466可顯著降低Glu含量,從而發(fā)揮神經(jīng)保護(hù)作用,且胎齡越小,效果越明顯。故應(yīng)用GYKI52466早期干預(yù)可望有效防治因早產(chǎn)、窒息導(dǎo)致新生兒腦損傷。
[Abstract]:Aim: to study the temporal compliance and intervention of glutamate (glutamate, Glu) content in brain tissue of neonatal hypoxic-ischemic encephalopathy (hypoxic ischemic encephalopathy, HIE) model with different gestational ages. Methods: 128 neonatal SD rats were divided into two groups according to their birth age (postnatal age, P): P2P6 P12P184 group with 32 rats in each group. According to different intervention measures, each group was divided into (hypoxic ischemic brain damage, HIBD) model control group, (H), 偽 -aminomethyl oxazolyl propionate (AMPA) intervention group, (A) GYKI52466 intervention group (G) and control group (N), 8 rats in each group. With the exception of group N, the HIBD standard model was established by Rice in each group. After 6 hours of administration, the contents of Glu in brain tissue were measured, and pathological examination was made at the same time. 緇撴灉錛氬悇鏃ラ緞緇勮剳緇勭粐涓璆lu鍚噺姣旇緝,Glu鍚噺涓庢棩榫勫憟璐熺浉鍏，

本文編號(hào)：2211424