本文選題：急性白血病 + 急性淋巴細胞白血病��； 參考：《山東醫(yī)藥》2017年35期

【摘要】：目的觀察急性白血病(AL)患兒骨髓單個核細胞中CAMP反應(yīng)元件型結(jié)合因子(CREB)、B淋巴細胞瘤-2(Bcl-2)mRNA和蛋白的表達變化,并探討其與AL患兒髓外浸潤、預(yù)后的關(guān)系。方法選擇2015年2月~2017年3月收治的96例AL患兒,其中急性淋巴細胞白血病(ALL)61例為ALL組,急性髓細胞白血病(AML)35例為對照組,另取骨髓穿刺排除白血病的其他血液病患兒35例為對照組。均行骨髓穿刺抽取骨髓組織分離單個核細胞,用實時熒光定量PCR法測算其中CREB、Bcl-2 mRNA相對表達量,用Western blotting法測算其中CREB、Bcl-2蛋白相對表達量,并進行組間比較。將ALL、AML組患兒按是否存在髓外浸潤分為髓外浸潤亞組和無髓外浸潤亞組,按化療后是否緩解分為緩解亞組和未緩解亞組,比較各亞組CREB、Bcl-2 mRNA和蛋白相對表達量。結(jié)果ALL、AML組骨髓單個核細胞中CREB、Bcl-2 mRNA和蛋白相對表達量均高于對照組(P均0.05)。ALL組和AML組骨髓單個核細胞CREB、Bcl-2 mRNA和蛋白相對表達量相比,P均0.05。ALL、AML組中有髓外浸潤亞組患兒骨髓單個核細胞中CREB、Bcl-2 mRNA和蛋白相對表達量均高于各自的無髓外浸潤亞組(P均0.05)。ALL、AML組中緩解亞組患兒骨髓單個核細胞中CREB、Bcl-2 mRNA和蛋白相對表達量均高于各自的未緩解亞組(P均0.05)。結(jié)論 AL患兒骨髓單個核細胞中CREB、Bcl-2 mRNA和蛋白高表達,且表達量與髓外浸潤和患兒預(yù)后有關(guān)。
[Abstract]:Objective to investigate the expression of camp responsive element binding factor (CREB) B lymphocyte tumor 2 (Bcl-2) mRNA and protein in bone marrow mononuclear cells of children with acute leukemia (AL), and to explore its relationship with extramedullary infiltration and prognosis. Methods from February 2015 to March 2017, 96 children with AL were selected. 61 patients with acute lymphoblastic leukemia (all) and 35 patients with acute myeloid leukemia (AML) were selected as control group. Another 35 children with other hematological diseases who were excluded from leukemia by bone marrow puncture were taken as control group. Bone marrow tissue was extracted from bone marrow to isolate mononuclear cells. The relative expression of CREBBcl 2 mRNA was measured by real-time fluorescence quantitative PCR, and the relative expression of CREBB 2 protein was measured by Western blotting method. The children with AML were divided into extramedullary infiltration subgroup and unmedullary infiltration subgroup according to the presence of extramedullary infiltration. According to the remission after chemotherapy, the children with AML were divided into remission subgroup and non-remission subgroup. The relative expression of CREBN Bcl-2 mRNA and protein in each subgroup was compared. Results the relative expression of CREBN Bcl-2 mRNA and protein in bone marrow mononuclear cells in AML group was higher than that in control group (P 0.05). The relative expression of CREBN Bcl-2 mRNA and protein in bone marrow mononuclear cells in all group and AML group was higher than that in all group and AML group. The relative expression of CREBB Bcl 2 mRNA and protein in mononuclear cells was higher than that in unmyelinated infiltrating subgroup (P 0.05). The relative expression of CREBN Bcl-2 mRNA and protein in bone marrow mononuclear cells in remission subgroup was higher than that in non-remission subgroup (P 0.05). Conclusion the expression of CREBN Bcl-2 mRNA and protein in bone marrow mononuclear cells of children with AL is high, and the expression level is related to extramedullary infiltration and prognosis.
【作者單位】： 山西醫(yī)科大學(xué)第二臨床醫(yī)學(xué)院;山西省婦幼保健院;
【基金】：山西省科技攻關(guān)項目(20120313020-6)
【分類號】：R733.71

【相似文獻】

相關(guān)期刊論文 前1條

1 王軍,趙雪蓮,馮建飛,張利,王偉,胡豫,王宏;再生障礙性貧血患兒骨髓單個核細胞c-kit受體的表達[J];鄭州大學(xué)學(xué)報(醫(yī)學(xué)版);2005年04期

，

本文編號：2117147