本文選題：Toll樣受體7 + 腸道病毒71型�。� 參考：《遵義醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的：探討腸道病毒71型(Enterovirus71，EV71)感染重癥患兒Toll樣受體7(Toll-likereceptor7，TLR7)及其基因多態(tài)性與抗病毒免疫、病情和預(yù)后的關(guān)系。 方法：研究對(duì)象包括深圳市兒童醫(yī)院收治的2012年至2013年EV71感染患兒115例（按病情分為重癥組和危重癥組），以及同期同年齡正常組嬰幼兒120例。提取研究對(duì)象外周血基因組DNA，PCR擴(kuò)增TLR7基因外顯子及啟動(dòng)子區(qū)序列，擴(kuò)增產(chǎn)物直接測序，分析測序結(jié)果；流式細(xì)胞術(shù)檢測外周血單核細(xì)胞（monocyte，MC）TLR7平均熒光強(qiáng)度表達(dá)；流式細(xì)胞術(shù)微球陣列法（cytometric bead array，CBA）檢測血漿IFN-α、IL-6、IL-10、IL-12等細(xì)胞因子的濃度。 結(jié)果：（1）18例EV71感染患兒中TLR7基因3號(hào)外顯子發(fā)現(xiàn)c.371TA位點(diǎn)突變，其基因型為半合子。（2）EV71感染危重癥患兒外周血MC TLR7平均熒光強(qiáng)度表達(dá)明顯高于同期同齡正常嬰幼兒（p0.01），，比較重癥患兒間TLR7平均熒光強(qiáng)度變化無明顯區(qū)別（p0.05）。（3）EV71感染患兒細(xì)胞因子IL-12及IFN-a水平明顯高于同期正常組嬰幼兒（p0.01），IL-6和IL-10細(xì)胞因子濃度無明顯改變（p0.05）。 結(jié)論：TLR7基因多態(tài)性可能參與了EV71感染的發(fā)病過程，TLR7基因3號(hào)外顯子c.371TA位點(diǎn)突變可能為EV71的易感基因。TLR7介導(dǎo)了EV71感染所致免疫功能的紊亂，引起EV71感染患兒IL-12及IFN-a細(xì)胞因子水平增高。
[Abstract]:Objective: to investigate the association of Toll-like receptor 7 (TLR7) gene polymorphism with antiviral immunity, condition and prognosis in severe children infected with enterovirus 71 (Enterovirus71). Methods: 115 children with EV71 infection were treated in Shenzhen Children's Hospital from 2012 to 2013 (divided into critical and critical groups according to their condition) and 120 infants of the same age in the same age group. The exons and promoters of TLR7 gene were amplified by PCR from peripheral blood genomic DNA, the products were sequenced directly and the results of sequencing were analyzed, and the mean fluorescence intensity of TLR7 in peripheral blood monocytes (monocyte MC) was detected by flow cytometry. Plasma levels of cytokines such as IL-6, IL-10 and IL-12 were measured by flow cytometry with (cytometric bead array. Results: (1) the mutation of c. 371TA in exon 3 of TLR7 gene was found in 18 children with EV71 infection. (2) the mean fluorescence intensity of peripheral blood MC TLR7 in severe children with EV71 infection was significantly higher than that in normal infants of the same age (p0.01). There was no significant difference in the mean fluorescence intensity of TLR7 between the severe children (p0.05). (3) and the children with EV71 infection (p0.05). (3). The levels of cytokines IL-12 and IFN-a were significantly higher than those of normal infants (p0.01) and the concentrations of IL-6 and IL-10 had no significant change (p0.05). Conclusion the gene polymorphism of TLR7 may be involved in the pathogenesis of EV71 infection. The mutation of c 371TA in exon 3 of TLR7 gene may be the susceptible gene of EV71. TLR7 mediates the disorder of immune function caused by EV71 infection. The levels of IL-12 and IFN-a cytokines were increased in children with EV 71 infection.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R725.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉亞敏;王春妍;宋立文;盛淑琴;;手足口病患兒體液免疫檢測分析[J];臨床和實(shí)驗(yàn)醫(yī)學(xué)雜志;2009年08期

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